A5088577485- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- SARS-CoV-2 detection and testing
- Hepatitis B Virus Studies
- Cancer Research and Treatments
- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Biosensors and Analytical Detection
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
Center of Molecular Immunology (Cuba)
2007-2021
University of Havana
2020
Subunit vaccines based on the receptor-binding domain (RBD) of spike protein SARS-CoV-2 provide one most promising strategies to fight COVID-19 pandemic. The detailed characterization primary structure by mass spectrometry (MS) is mandatory, as described in ICHQ6B guidelines. In this work, several recombinant RBD proteins produced five expression systems were characterized using a non-conventional protocol known in-solution buffer-free digestion (BFD). single ESI-MS spectrum, BFD allowed...
Enzyme-based electrochemical sensors are important tools for the detection of numerous analytes biological origin. The immobilization enzymes onto electrodes is a critical step during fabrication these devices. Herein, we report glutamate dehydrogenase (GDH)-based biosensors using two different strategies, and effect method on performances obtained bioelectrodes. first consisted in cross-linking GDH surface with glutaraldehyde. as presented drawbacks including (i) complete loss enzyme...
Abstract Subunit vaccines based on the receptor-binding domain (RBD) of spike protein SARS-CoV-2, are among most promising strategies to fight COVID-19 pandemic. The detailed characterization primary structure by mass spectrometry (MS) is mandatory, as described in ICHQ6B guidelines. In this work, several recombinant RBD proteins produced five expression systems were characterized using a non-conventional protocol known in-solution buffer-free digestion (BFD). single ESI-MS spectrum, BFD...
Abstract The high prices of biopharmaceuticals or biologics used in the treatment many diseases limit access patients to these novel therapies. One example is monoclonal antibody trastuzumab, successfully for breast cancer treatment. An economic alternative generation biosimilars expensive biopharmaceuticals. Since therapies may require large doses over a long period time, robust platforms and strategies cell line development are essential recombinant lines with higher levels expression....
Abstract The high prices of biopharmaceutics or biologics used in the treatment many diseases limit access patients to these novel therapies. One example is monoclonal antibody trastuzumab, successfully for breast cancer treatment. An economic alternative generation biosimilars expensive biopharmaceutics. Since therapies may require large doses over a long period time, robust platforms and strategies cell line development are essential recombinant lines short time with higher levels...