A5041757816- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Alzheimer's disease research and treatments
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Neurobiology and Insect Physiology Research
- Advanced Sensor and Energy Harvesting Materials
- Advanced biosensing and bioanalysis techniques
- Terahertz technology and applications
- Nuclear Receptors and Signaling
- Hippo pathway signaling and YAP/TAZ
- Cardiac Ischemia and Reperfusion
- Bacteriophages and microbial interactions
- Advanced Memory and Neural Computing
- Fuel Cells and Related Materials
- bioluminescence and chemiluminescence research
- Axon Guidance and Neuronal Signaling
- Blood Coagulation and Thrombosis Mechanisms
- Viral Infections and Immunology Research
- CO2 Reduction Techniques and Catalysts
- Retinal Development and Disorders
- Phytoestrogen effects and research
- Molecular Communication and Nanonetworks
Beihang University
2007-2025
Xi'an Technological University
2024
Beijing Advanced Sciences and Innovation Center
2020
Tsinghua University
2014-2018
University of Washington
2001-2004
Geriatric Research Education and Clinical Center
2004
University of Puget Sound
2004
Southern Illinois University School of Medicine
1997-1999
Abstract GCaMP, one popular type of genetically-encoded Ca 2+ indicator, has been associated with various side-effects. Here we unveil the intrinsic problem prevailing over different versions and applications, showing that GCaMP containing CaM (calmodulin) interferes both gating signaling L-type calcium channels (Ca V 1). acts as an impaired apoCaM /CaM, critical to 1, which disrupts dynamics gene expression. We then design implement GCaMP-X, by incorporating extra apoCaM-binding motif,...
Abstract Mammalian voltage‐gated calcium channels (Ca V ) play critical roles in cardiac excitability, synaptic transmission, and gene transcription. Dysfunctions Ca are implicated a variety of neurodevelopmental disorders. Current pharmacological approaches to enhance activity limited by off‐target effects, drug metabolism issues, cytotoxicity, imprecise modulation. Additionally, genetically‐encoded channel activators optogenetic tools restricted delivery challenges biosafety concerns. Here...
Genistein is a potent plant-derived isoflavone displaying estrogenic activity at low (nanomolar) concentrations and antiproliferative antiangiogenic properties higher (above 10-50 microM). The potential of genistein has made it an interesting candidate for cancer chemotherapy high concentrations; however, the toxicity in neurons such not been previously addressed. We show that toxic to rat primary cortical concentration 50 microM, whereas daidzein, structural analog, shows no similar...
Cations such as K+ play a key part in the CO2 electroreduction reaction, but their role reaction mechanism is still debate. Here, we use highly symmetric Ni-N4 structure to selectively probe mechanistic influence of and identify its interaction with chemisorbed CO2−. Our electrochemical kinetics study finds shift rate-determining step presence K+. Spectral evidence CO2− from in-situ X-ray absorption spectroscopy Raman pinpoints origin this shift. Grand canonical potential simulations -...
Dynamic Ca
Abstract : The transcriptional mechanism underlying amyloid precursor protein (APP) regulation in primary neurons during development was investigated. We observed an approximately threefold elevation of APP mRNA levels differentiating rat hippocampal between day 1 and 7 culture brain hippocampi embryonic 18 postnatal 3. When promoter construct extending to position ‐2,832 upstream from the main start site transfected into neurons, activity increased until reaching a maximum on culture. This...
Sequential proteolysis of the amyloid precursor protein (APP) by औ- and γ-secretase activities yields औ peptide that is widely deposited in brains individuals with Alzheimer's disease. The membrane-anchored aspartyl protease औ-siteAPP-cleaving enzyme (BACE) exhibits all characteristics a औ-secretase has been shown to cleave APP at its औ-site vitro vivo. We found BACE undergoes cleavage on surface-exposed α-helix between amino acid residues Leu-228 Ala-229, generating stable N- C-terminal...
Inhibitions and antagonists of L-type Ca2+ channels are important to both research therapeutics. Here, we report C-terminus mediated inhibition (CMI) for CaV1.3 that multiple motifs coordinate tune down current influx toward the lower limits determined by end-stage CDI (Ca2+-dependent inactivation). Among IQV (preIQ3-IQ domain), PCRD DCRD (proximal or distal C-terminal regulatory spatial closeness any two modules, e.g., constitutive fusion, facilitates trio form complex, compete against...
Abstract L-type Ca 2+ (Ca V 1) channels transduce channel activities into nuclear signals critical to neuritogenesis. Also, standalone peptides encoded by 1 DCT (distal carboxyl-terminus) act as transcription factors reportedly promoting Here, focusing on exemplary 1.3 and cortical neurons under basal conditions, we discover that cytosolic downregulate neurite outgrowth the interactions with 1’s apo-calmodulin binding motif. Distinct from DCT, various exert a gradient of inhibitory effects...
Abstract Glutamate uptake by high affinity glutamate transporters is essential for preventing excitotoxicity and maintaining normal synaptic function. We have discovered a novel role presenilin‐1 (PS1) as regulator of transport. PS1‐deficient neurons showed decrease in approximately 50% compared to wild‐type neurons. Gamma‐secretase inhibitor treatment mimicked the effects PS1 deficiency on uptake. loss‐of‐function, accomplished or γ‐secretase treatment, caused corresponding cell surface...
Extracellular S3-S4 linkers of domain IV (IVS3-S4) L-type Ca2+ channels (CaV1) are subject to alternative splicing, resulting into distinct gating profiles serving for diverse physiological roles. However, it has remained elusive what would be the determining factor IVS3-S4 effects on CaV1 channels. In this study, we systematically compared variants from CaV1.1-1.4, and discover that flexibility linker plays a prominent role in characteristics. Chimeric analysis mutagenesis demonstrated...
Phase transition process occurs rapidly and at unpredictable time, posing challenges for traditional cameras to capture the entire high speed. Here, we present a self-supervised learning enhanced high-speed phase imaging method based on spike streams.