A5043122127- Plant biochemistry and biosynthesis
- Plant and animal studies
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Pesticide and Herbicide Environmental Studies
- Essential Oils and Antimicrobial Activity
- Transgenic Plants and Applications
- Horticultural and Viticultural Research
- Plant Parasitism and Resistance
- Biochemical and biochemical processes
- Toxin Mechanisms and Immunotoxins
- Antioxidant Activity and Oxidative Stress
- Plant Physiology and Cultivation Studies
- Phytochemistry and Biological Activities
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Powdery Mildew Fungal Diseases
- Plant tissue culture and regeneration
- Fermentation and Sensory Analysis
- Plant Pathogens and Fungal Diseases
- Olfactory and Sensory Function Studies
- Natural product bioactivities and synthesis
- Microbial Metabolic Engineering and Bioproduction
- French Urban and Social Studies
Laboratoire de Biotechnologies Végétales Appliquées aux Plantes Aromatiques et Médicinales
2016-2025
Université Jean Monnet
2011-2025
Centre National de la Recherche Scientifique
2001-2025
Université Claude Bernard Lyon 1
2014-2023
Normandie Université
2015
Université de Rouen Normandie
2015
Swedish University of Agricultural Sciences
2011
Laboratoire Reproduction et Développement des Plantes
2005
Institut National de la Recherche Agronomique
2005
Centre National pour la Recherche Scientifique et Technique (CNRST)
2005
Roses have high cultural and economic importance as ornamental plants in the perfume industry. We report rose whole-genome sequencing assembly resequencing of major genotypes that contributed to domestication. generated a homozygous genotype from heterozygous diploid modern progenitor, Rosa chinensis 'Old Blush'. Using single-molecule real-time meta-assembly approach, we obtained one most comprehensive plant genomes date. Diversity analyses highlighted mosaic origin 'La France', first...
The scent of roses (Rosa x hybrida) is composed hundreds volatile molecules. Monoterpenes represent up to 70% percent the content in some cultivars, such as Papa Meilland rose. Monoterpene biosynthesis plants relies on plastid-localized terpene synthases. Combining transcriptomic and genetic approaches, we show that Nudix hydrolase RhNUDX1, localized cytoplasm, part a pathway for free monoterpene alcohols contribute fragrance roses. RhNUDX1 protein shows geranyl diphosphate...
Roses have been cultivated for centuries and a number of varieties selected based on flower traits such as petal form, color, number. Wild-type roses five petals (simple flowers), whereas high numbers (double flowers) are typical attributes most the roses. Here, we investigated molecular mechanisms that could to control in
Geraniol derived from essential oils of various plant species is widely used in the cosmetic and perfume industries. It also an trait pleasant smell rose flowers. In contrast to other monoterpenes which are produced plastids via methyl erythritol phosphate pathway, geraniol biosynthesis roses relies on cytosolic NUDX1 hydrolase dephosphorylates geranyl diphosphate (GPP). However, metabolic origin GPP remains unknown. By feeding Rosa chinensis "Old Blush" flowers with pathway-specific...
The phenolic methyl ether 3,5-dimethoxytoluene (DMT) is a major scent compound of many modern rose varieties, and its fragrance participates in the characteristic “tea scent” that gave their name to Tea Hybrid roses. Among wild roses, (PME) biosynthesis restricted Chinese species, but progenitors roses included both European species (e.g., Rosa chinensis cv Old Blush), so this trait was transmitted hybrid progeny. last steps biosynthetic pathways leading DMT involve two methylation reactions...
The recent completion of the rose genome sequence is not end a process, but rather starting point that opens up whole set new and exciting activities. Next to high-quality other genomic tools have also become available for rose, including transcriptomics data, high-density single-nucleotide polymorphism array software perform linkage quantitative trait locus mapping in polyploids. Rose cultivars are highly heterogeneous diverse. This vast diversity cultivated roses can be explained through...
Abstract The fascinating scent of rose (Rosa genus) flowers has captivated human senses for centuries, making them one the most popular and widely used floral fragrances. Despite much progress over last decade, many biochemical pathways responsible scents remain unclear. We analyzed compositions from various varieties selected modern cultivar Rosa hybrida “Double Delight” as a model system to unravel formation dominant volatile terpenes, which contribute substantially fragrance. Key genes...
Abstract Background For centuries roses have been selected based on a number of traits. Little information exists the genetic and molecular basis that contributes to these traits, mainly because expressed genes for this economically important ornamental plant is scarce. Results Here, we used combination Illumina 454 sequencing technologies generate Rosa sp. transcripts using RNA from various tissues in response biotic abiotic stresses. A total 80714 transcript clusters were identified 76611...
Nudix hydrolases are conserved enzymes ubiquitously present in all kingdoms of life. Recent research revealed that several involved terpenoid metabolism plants. In modern roses, RhNUDX1 is responsible for formation geraniol, a major compound rose scent. Nevertheless, this produced by monoterpene synthases many geraniol-producing As consequence, raised the question about origin function and NUDX1 gene evolution Rosaceae, wild roses or/and during domestication process. Here, we showed three...
In Chinese rose species and in many modern varieties, two methylated phenolic derivatives, 3,5‐dimethoxytoluene 1,3,5‐trimethoxybenzene, are major scent components. We show that cell‐free extracts of petals catalyse the synthesis 1,3,5‐trimethoxybenzene by methylation precursor molecules. An expressed sequence tag approach was used to identify four highly similar O ‐methyltransferase sequences specifically anthers. Thin layer chromatography analysis showed activities these enzymes with...
Abstract Orcinol O-methyltransferase (OOMT) 1 and 2 catalyze the last two steps of biosynthetic pathway leading to phenolic methyl ether 3,5-dimethoxytoluene (DMT), major scent compound many rose (Rosa x hybrida) varieties. Modern roses are descended from both European Chinese species, latter being producers ethers but not former. Here we investigated why production occurs in some all In DMT-producing varieties, OOMTs were shown be localized specifically petal, predominanty adaxial epidermal...
Nowadays, the combination of molecules influences their biological effects, and interesting outcomes can be obtained from different component interactions. Using a mixture design method, this research seeks to simulate efficacy essential oil combinations against various bacteria forecast ideal combination. The chemical compositions Myrtus communis, Artemisia herba-alba Thymus serpyllum oils were analyzed using CG/MS. Then, combined antibacterial effects evaluated by testing formulations...
• Floral scents and visual cues of the globeflower Trollius europaeus may play a key role in attraction Chiastocheta flies, involved highly specific nursery pollination mutualism. Here, headspace collection GC-MS were used to identify quantify volatile organic compounds emitted by globeflower. Scents are produced three different floral parts four structures: secretory glands flat epidermis cells abaxial sepal epidermis, conical adaxial pollen. The blend is made up 16 commonly found scents....
Sclareol is a high-value natural product obtained by solid/liquid extraction of clary sage (Salvia sclarea L.) inflorescences. Because processes excretion and accumulation this labdane diterpene are unknown, the aim work was to gain knowledge on its sites in planta. Samples were collected natura or during different steps industrial process (steam distillation extraction). then analysed with combination complementary analytical techniques (gas chromatography coupled mass spectrometer,...