- Plant Gene Expression Analysis
- Postharvest Quality and Shelf Life Management
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Plant Physiology and Cultivation Studies
- Berry genetics and cultivation research
- Horticultural and Viticultural Research
- Biofuel production and bioconversion
- Phytochemicals and Antioxidant Activities
- Light effects on plants
- Fermentation and Sensory Analysis
- Tree Root and Stability Studies
- Plant Pathogens and Fungal Diseases
- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Nanocomposite Films for Food Packaging
- Molecular Biology Techniques and Applications
- Botanical Research and Applications
- Advanced Cellulose Research Studies
- Plant Stress Responses and Tolerance
- Plant Surface Properties and Treatments
- Plant nutrient uptake and metabolism
- Amphibian and Reptile Biology
- Photosynthetic Processes and Mechanisms
University of Talca
2015-2025
Baptist Health South Florida
2022-2024
Agencia Nacional de Investigación y Desarrollo
2024
Universidad Autónoma de Coahuila
2016-2022
Baylor School
2022
New York University
2008-2019
Stony Brook University
2019
Unidad de Cirugía Cardiovascular de Guatemala
2018
Universidad Carlos III de Madrid
2017
Instituto de Salud del Estado de México
2016
A high-throughput genotyping platform is needed to enable marker-assisted breeding in the allo-octoploid cultivated strawberry Fragaria × ananassa. Short-read sequences from one diploid and 19 octoploid accessions were aligned vesca 'Hawaii 4' reference genome identify single nucleotide polymorphisms (SNPs) indels for incorporation into a 90 K Affymetrix® Axiom® array. We report development preliminary evaluation of this About 36 million sequence variants identified member, germplasm panel....
A large quantity of high quality RNA is often required in the analysis gene expression.However, extraction from samples taken woody plants generally complex, and represents main limitation to study expression, particularly refractory species like conifers.Standard protocols are available but they highly time consuming, not adapted scale extraction.Here we present a high-throughput protocol.This protocol was micro-scale by modifying classical cetyltrimethylammonium (CTAB) developed for pine:...
Phenolic compounds with antioxidant properties have risen in interest due to their benefits for human health. Fragaria chiloensis is a native wild berry species from Chile that develops white/pink receptacle and white flesh at the ripe stage. Changes color parameters, anthocyanins, secondary metabolites (phenolics, flavonoids), total capacity were followed during development ripening of F. fruit. The increment 'a' index takes place parallel anthocyanins rise reduction phenolics, flavonoids,...
Fragaria chiloensis is characterized for having great aroma and flavor properties. Using headspace-SPME different volatile compounds were identified quantified during development ripening of the fruit. The headspace was dominated by esters, butyl acetate, ethyl butanoate hexanoate being most abundant in fully ripe As esters are important synthesized through alcohol acyltransferases (AAT), a full-length cDNA (FcAAT1) isolated from F. fruit which displayed three motifs characteristic AATs....
Mountain papaya ( Vasconcellea pubescens ) is a climacteric fruit that develops strong and characteristic aroma during ripening. Esters are the main volatile compounds produced by fruit, most of them dependent on ethylene. As esters synthesized through alcohol acyltransferases (AAT), full-length cDNA (VpAAT1) was isolated displayed motifs plant acyltransferases. The sequence cloned expressed in yeasts, obtaining functional enzyme with high AAT activity toward formation benzyl acetate....
Fragaria chiloensis is a strawberry endemic from Chile with attractive white-pink fruit, pleasant aroma and taste. However, this fruit has limited post-harvest period due to fast softening. Several transcription factors (TFs) are involved in the regulation of ripening, members NAC family have been implicated cell wall remodeling. FcNAC1 was isolated F. coding protein 332 amino acid residues displaying characteristic domain at N terminus. showed nuclear localization. An increase transcript...
Alcohol acyltransferases (AAT) play a key role in ester biosynthesis. In Cucumis melo var. cantalupensis, AATs are encoded by gene family of four members (CmAAT1-4). CmAAT1, CmAAT3 and CmAAT4 capable synthesizing esters, with CmAAT1 the most active. CmAAT2 is inactive has an Ala268 residue instead threonine which present all other active AATs, although this still unclear. The work aims to understand molecular mechanism involved biosynthesis melon fruit clarify importance residue. First,...
Fragaria vesca or 'woodland strawberry' has emerged as an attractive model for the study of ripening non-climacteric fruit. It several advantages, such its small genome and diploidy. The recent availability complete sequence opens possibility further analysis use a reference species. Fruit softening is physiological event involves many biochemical changes that take place at final stages fruit development; among them, remodeling cell walls by action set enzymes. Xyloglucan...
Chilean papaya, also known as mountain papaya ( Vasconcellea pubescens ), is a fruit valued for its nutritional value and pleasant fragrance. The oblong fruit, featuring five ridges seed-filled mucilage cavity, typically consumed cooked due to high protease content. the seeds are usually discarded byproducts. This study analyzed biochemical composition of seed using methods such HPAEC immunolabeling. Results revealed that yield nearly 20% their weight in polysaccharides, which can be...
Several previous studies have established that the introduction of American bullfrog, Lithobates catesbeianus, produces negative effects on native communities. Herein, bullfrog is reported for first time in province Buenos Aires, Argentina locality 9 de Julio. The stomach contents collected specimens were examined and reproduction species was also confirmed at study area. A total 85.7% (n = 30) analyzed stomachs contained prey items, eleven these identified. items belonged mostly to...