A5103082823- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Single-cell and spatial transcriptomics
- Angiogenesis and VEGF in Cancer
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Developmental Biology and Gene Regulation
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- CAR-T cell therapy research
- Graph Labeling and Dimension Problems
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer-related molecular mechanisms research
- T-cell and B-cell Immunology
- Advanced Differential Equations and Dynamical Systems
- Retinal Development and Disorders
- Advanced Mathematical Theories
- Aquaculture disease management and microbiota
- Hippo pathway signaling and YAP/TAZ
- Axon Guidance and Neuronal Signaling
- Cell Image Analysis Techniques
- Rings, Modules, and Algebras
- Mathematics and Applications
University of Massachusetts Chan Medical School
2011-2017
Massachusetts General Hospital
2014-2016
Harvard Stem Cell Institute
2014-2016
Princeton University
1957
Differentiation of arteries and veins is essential for the development a functional circulatory system. In vertebrate embryos, genetic manipulation Notch signaling has demonstrated importance this pathway in driving artery endothelial cell differentiation. However, when where activation occurs to affect fate less clear. Using transgenic zebrafish bearing Notch-responsive reporter, we demonstrate that activated progenitors during vasculogenesis prior blood vessel morphogenesis maintained...
Abstract Cancers contain a wide diversity of cell types that are defined by differentiation states, genetic mutations and altered epigenetic programmes impart functional to individual cells. Elevated tumour heterogeneity is linked with progression, therapy resistance relapse. Yet, imaging the hallmarks cancer has been technical biological challenge. Here we develop optically clear immune-compromised rag2 E450fs (casper) zebrafish for optimized transplantation direct visualization...
Hematopoiesis culminates in the production of functionally heterogeneous blood cell types. In zebrafish, lack surface antibodies has compelled researchers to use fluorescent transgenic reporter lines label specific fractions. However, these approaches are limited by availability and protein combinations that can be distinguished. Here, we have transcriptionally profiled single hematopoietic cells from zebrafish define erythroid, myeloid, B, T lineages. We also used our approach identify stem...
Cell transplantation into immunodeficient mice has revolutionized our understanding of regeneration, stem cell self-renewal, and cancer; yet models for direct imaging engrafted cells been limited. Here, we characterize zebrafish with mutations in recombination activating gene 2 (rag2), DNA-dependent protein kinase (prkdc), janus 3 (jak3). Histology, RNA sequencing, single-cell transcriptional profiling blood showed that rag2 hypomorphic mutant lack T cells, whereas prkdc deficiency results...
ZAP70 [zeta-chain (TCR)-associated protein kinase, 70-kDa], is required for T cell activation. deficiencies in humans and null mutations mice lead to severe combined immune deficiency. Here, we describe a zap70 loss-of-function mutation zebrafish (zap70y442) that was created using transcription activator-like effector nucleases (TALENs). In contrast what has been reported morphant zebrafish, zap70y442 homozygous mutant displayed normal development of blood lymphatic vasculature....
A variety of signaling pathways have been shown to regulate specification neuronal subtype identity. However, the mechanisms by which future neurons simultaneously process information from multiple establish their identity remain poorly understood. The zebrafish pineal gland offers a simple system with address questions concerning integration during neural as it contains only two types – photoreceptors and projection neurons. We previously that Notch inhibits neuron fate. Here, we show BMP...
Zebrafish have become a powerful tool for assessing development, regeneration, and cancer. More recently, allograft cell transplantation protocols been developed that permit engraftment of normal malignant cells into irradiated, syngeneic, immune compromised adult zebrafish. These models when coupled with optimized allow the rapid assessment stem function, regeneration following injury, Here, we present method zebrafish skeletal muscle embryonal rhabdomyosarcoma (ERMS), pediatric sarcoma...
The lack of myostatin promotes growth skeletal muscle, and blockade its activity has been proposed as a treatment for various muscle-wasting disorders. Here, we have examined two independent mouse lines that harbor mutations in the gene, ...
Abstract Cell transplantation into immune compromised mice has transformed our understanding of cancer and is now the gold standard for assessing therapeutic responses in vivo. However, mouse models are expensive engraftment often difficult to visualize directly. To overcome these challenges, we have developed zebrafish (ICZ) transparent casper background using genome editing techniques. We successfully targeted genes required cell function well known cause deficiency human mice. date,...