A5034225805- Cancer, Stress, Anesthesia, and Immune Response
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Anesthesia and Pain Management
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Anesthesia and Neurotoxicity Research
- Phagocytosis and Immune Regulation
- Neuropeptides and Animal Physiology
- Cellular transport and secretion
- Immune Cell Function and Interaction
- Adenosine and Purinergic Signaling
- Cancer Research and Treatments
- Cardiac, Anesthesia and Surgical Outcomes
- RNA regulation and disease
- Enhanced Recovery After Surgery
- Lung Cancer Treatments and Mutations
- Cannabis and Cannabinoid Research
- Immune cells in cancer
- Photodynamic Therapy Research Studies
- CAR-T cell therapy research
- Intensive Care Unit Cognitive Disorders
- Antimicrobial Peptides and Activities
Institut Gustave Roussy
2016-2025
European Society of Anaesthesiology
2022-2025
Sorbonne Université
2015-2024
Université Paris Cité
2015-2024
Institut Universitaire de France
2020-2024
Hôpital Européen
2019-2024
Centre de Recherche des Cordeliers
2015-2024
La Ligue Contre le Cancer
2015-2024
Université Paris-Saclay
2018-2024
NHS Grampian
2024
Abstract Immunogenic cell death (ICD) converts dying cancer cells into a therapeutic vaccine and stimulates antitumor immune responses. Here we unravel the results of an unbiased screen identifying high-dose (10 µM) crizotinib as ICD-inducing tyrosine kinase inhibitor that has exceptional antineoplastic activity when combined with non-ICD inducing chemotherapeutics like cisplatin. The combination cisplatin induces ICD in non-small lung carcinoma (NSCLC) effectively controls growth distinct...
LTX-315 is a cationic amphilytic peptide that preferentially permeabilizes mitochondrial membranes, thereby causing partially BAX/BAK1-regulated, caspase-independent necrosis. Based on the observation intratumorally injected stimulates strong T lymphocyte-mediated anticancer immune response, we investigated whether may elicit hallmarks of immunogenic cell death (ICD), namely (i) exposure calreticulin plasma membrane surface, (ii) release ATP into extracellular space, (iii) exodus HMGB1 from...
Article23 April 2020Open Access Transparent process Inhibition of transcription by dactinomycin reveals a new characteristic immunogenic cell stress Juliette Humeau Equipe labellisée par la Ligue contre le Cancer, Sorbonne Université, INSERM UMR1138, Centre de Recherche des Cordeliers, Université Paris, France Metabolomics and Cell Biology Platforms, Gustave Roussy, Villejuif, Faculty Medicine Kremlin Bicêtre, Paris Sud, Saclay, Search for more papers this author Allan Sauvat Giulia Cerrato...
Immunogenic cell death (ICD) induced by anticancer chemotherapeutics is usually preceded premortem stress affecting the endoplasmic reticulum (ER). This ER does not reflect a canonical unfolded protein response (UPR) but rather manifests solely at level of phosphorylation eIF2α. eIF2α hence quintessential hallmark ICD that can be detected immunohistochemistry in tumor samples.
The translocation of the protein high mobility group box 1 (HMGB1) from nucleus to cytoplasm and its secretion or passive release through permeabilized plasma membrane, constitutes a major cellular danger signal. Extracellular HMGB1 can interact with pattern recognition receptors stimulate pro-inflammatory immunostimulatory pathways. Here, we developed screening assay identify pharmacological agents endowed releasing properties. For this, took advantage "retention using selective hooks"...
Background Retrospective clinical trials reported a reduced local relapse rate, as well improved overall survival after injection of anesthetics during cancer surgery. Here, we investigated the anticancer effects six used in practice. Results In vitro , induced signs cell stress including inhibition oxidative phosphorylation, and induction autophagy endoplasmic reticulum (ER) characterized by splicing X-box binding protein 1 (XBP1s) mRNA, cleavage activating transcription factor 6 (ATF6),...
Preclinical evidence indicates potent antitumor properties of local anesthetics. Numerous underlying mechanisms explaining such anticancer effects have been identified, suggesting direct cytotoxic as well indirect immunemediated that together reduce the proliferative, invasive and migratory potential malignant cells. Although some retrospective correlative studies support these findings, prospective randomized controlled trials not yet fully confirmed antineoplastic activity anesthetics,...
Depending on the length of their carbon backbone and saturation status, natural fatty acids have rather distinct biological effects. Thus, longevity model organisms is increased by extra supply most abundant cis-unsaturated acid, oleic but not that saturated palmitic acid. Here, we systematically compared capacity different saturated, alien (industrial or ruminant) trans-unsaturated to provoke cellular stress in vitro, cultured human cells expressing a battery biosensors detect signs...
Different intrinsic and extrinsic stress pathways including endoplasmic reticulum (ER) converge on the phosphorylation of eukaryotic translation initiation factor 2A (EIF2A, best known as eIF2α), which characterizes so-called "integrated response". This event is important for induction autophagy in response to multiple distinct stressors, well exposure calreticulin (CALR) an "eat me" signal surface plasma membrane stressed cells. Both CALR are required immunogenic cell death, a modality...
Compelling evidence supports the hypothesis that stress negatively impacts cancer development and prognosis. Irrespective of its physical, biological or psychological source, triggers a physiological response is mediated by hypothalamic-pituitary-adrenal axis sympathetic adrenal medullary axis. The resulting release glucocorticoids catecholamines into systemic circulation leads to neuroendocrine metabolic adaptations can affect immune homeostasis immunosurveillance, thus impairing detection...
The oncolytic peptide LTX-315 has been designed for killing human cancer cells and turned out to stimulate anti-cancer immune responses when locally injected into tumors established in immunocompetent mice. Here, we investigated the question whether induces apoptosis or necrosis. Transmission electron microscopy morphometric analysis of chromatin-stained tumor revealed that failed induce apoptotic nuclear condensation rather induced a necrotic phenotype. Accordingly, activation caspase-3,...
Abstract Salicylate, the active derivative of aspirin (acetylsalicylate), recapitulates mode action caloric restriction inasmuch as it stimulates autophagy through inhibition acetyltransferase activity EP300. Here, we directly compared metabolic effects medication with those elicited by 48 h fasting in mice, revealing convergent alterations plasma and heart metabolome. Aspirin caused a transient reduction general protein acetylation blood leukocytes, accompanied induction autophagy. However,...