Abstract Objective To determine the magnetic resonance imaging (MRI), macroscopic, and microscopic characteristics of synovial membrane inflammation, to study relationship between disease severity degree inflammation on MRI macroscopic examination, look for colocalization chondral lesions inflammation. Methods Thirty‐nine patients with knee osteoarthritis (OA) were classified into 2 groups according cartilage as revealed by chondroscopy. Group 1 (n = 14) had mild lesion(s) without exposure...

Abstract Developing methods for accurate detection of RNA modifications remains a major challenge in epitranscriptomics. Next-generation sequencing-based mapping approaches have recently emerged but, often, they are not quantitative and lack specificity. Pseudouridine (ψ), produced by uridine isomerization, is one the most abundant modification. ψ classically involves derivatization with soluble carbodiimide (CMCT), which prone to variation making this approach only semi-quantitative. Here,...

Mesenchymal stem cells (MSCs) are found in synovial fluid (SF) and can easily be harvested during arthrocentesis or arthroscopy. However, SF-MSC characterization chondrogenicity collagen sponges have been poorly documented as well their hypothetical vivo chondroprotective properties with intra-articular injections experimental osteoarthritis (OA). SF-MSCs were isolated from human SF aspirates patients suffering advanced OA undergoing total knee joint replacements. at passage 2 (P2)...

Abstract Background Due to their intrinsic properties, stem cells are promising tools for new developments in tissue engineering and particularly cartilage regeneration. Although mesenchymal stromal/stem from bone marrow (BM-MSC) have long been the most used cell source engineering, they certain limits. Thanks properties such as low immunogenicity chondrogenic differentiation potential, Wharton’s jelly (WJ-MSC) promise be an interesting of MSC engineering. Methods In this study, we propose...

PURPOSE: To evaluate experimentally the sensitivity of T2 mapping with magnetic resonance (MR) imaging at 8.5 T in depicting variations proteoglycan content and concurrent extracellular matrix rat patellar cartilage. MATERIALS AND METHODS: The study was performed 36 immature (age, 5 weeks) mature 10 Wistar rats. Maintenance care rats were conducted accordance National Institutes Health guidelines. Fifty-six underwent 28 right patellae degraded hyaluronidase for 1 6 hours undegraded...

Aim The aim of this work was the development successful cell therapy techniques for cartilage engineering. This will depend on ability to monitor non-invasively transplanted cells, especially mesenchymal stem cells (MSCs) that are promising candidates regenerate damaged tissues. Methods MSCs were labeled with superparamagnetic iron oxide particles (SPIO). We examined effects long-term labeling, possible toxicological consequences and influence progressive concentrations SPIO chondrogenic...

3D bioprinting offers interesting opportunities for tissue printing by providing living cells with appropriate scaffolds a dedicated structure. Biological advances in bioinks are currently promising cell encapsulation, particularly that of mesenchymal stem (MSCs). We present herein the development cartilage implants deliver MSCs encapsulated an original bioink at low concentration. 3D-bioprinted constructs (10 × 10 4 mm) were printed using alginate/gelatin/fibrinogen mixed human bone marrow...

Osteoarthritis is characterized by a gradual degradation of extracellular matrix, resulting from an excess chondrocyte cell death, mainly due to increase in apoptotis. Recent studies have revealed the essential role HSP70 protecting cells stressful stimuli. Therefore, overexpressing chondrocytes could represent good strategy prevent matrix destruction. To this end, we developed vector carrying HSP70/GFP, and transduced were thus more resistant death induced mono-iodoacetate (MIA). overcome...

Both chondrocytes and mensenchymal stem cells (MSCs) are the most used cell sources for cartilage tissue engineering. However, monolayer expansion to obtain sufficient leads a rapid chondrocyte dedifferentiation subsequent ancillary reduced ability of MSCs differentiate into chondrocytes, thus limiting their application in repair. The aim this study was investigate influence on immunophenotype gene expression profile both types, find appropriate compromise between remaining chondrogenic...

Cartilage tissue engineering strategies generally result in homogeneous structures with little resemblance to native zonal organization of articular cartilage. The main objective our work concerns the buildup complex biomaterials aimed at reconstructing biological three dimensional cells construction for mimicking cartilage architecture.

Tissue engineering is a multidisciplinary field that applies the principles of engineering, life sciences, cell and molecular biology toward development biological substitutes restore, maintain, improve tissue function. In Western Cou

Objective. To assess OA-related changes in mean compartmental femorotibial cartilage thickness rat knees by three-dimensional (3D) MRI (7T). Methods. was performed vivo at 7T on OA and untouched contralateral knee joints. Gradient Echo Fast Imaging 3D MR images were acquired sequentially surgically induced (D0) 40 Wistar rats (anterior cruciate ligament transection). Mean femoral (trochlear, lateral medial) tibial (lateral thicknesses quantified from a 2D slide weight-bearing areas data set....

Nacre (or mother of pearl) can facilitate bone cell differentiation and speed up their mineralization. Here we report on the capability nacre to induce human marrow mesenchymal stem cells (hBM-MSCs) production extracellular matrix. hBM-MSCs were encapsulated in an alginate hydrogel containing different concentrations powdered cultured same environment until Day 28. Analysis osteogenic gene expression, histochemistry, second harmonic generation (SHG) microscopy, Raman scattering spectroscopy...

MSCs isolated from bone marrow (BM-MSCs) have well-established chondrogenic potential, but derived the synovial membrane (SM-MSCs) and fluid (SF-MSCs) are thought to possess superior chondrogenicity. This study aimed compare in vitro immunophenotype trilineage potential of BM-MSCs SM-MSCs SF-MSCs.MSCs were (BM-MSCs), (SM-MSCs), extracted hips (BM) knees (SM SF) advanced OA patients undergoing arthroplasty. Flow cytometric analysis was used at P2 evaluate cell stemness. The trilinear...

We have taken advantage of the large screening capacity a multiplex immunoassay to better define respective contribution articular versus systemic cytokines in experimental arthritis. performed follow up (from 7 hours 14 days) analysis 24 synovial fluid and sera rats developing Antigen-Induced Arthritis (AIA) confronted their protein level changes with molecular, biochemical, histological clinical events occurring course disease. The time-scheduled findings arthritic joints correlated...

We examined the respective influence of a sequential or continuous hypoxia during expansion and transforming growth factor beta 1-driven chondrogenic differentiation human bone marrow mesenchymal stem cells (MSCs). The was performed within alginate beads, classical tool for implantation MSCs joint. standard normoxic 2D (expansion) 3D (differentiation) cultures served as reference. To determine quality chondrogenesis, we analyzed typical markers such type II X collagens, SOX9, COMP, versican,...

The aim of this work was to determine whether Hsp70 overexpression via proteasome inhibitor MG132 able protect chondrocytes towards mono‐iodoacetate (MIA) cytotoxicity both in vitro and vivo. In vitro, significantly from MIA toxicity (MTT/LDH analyses). essentially mediated chondroprotective effect as demonstrated by antisense strategy. vivo, chondrocytic Hsp70, after a preventive intra‐articular injection rat knee, sufficient decrease the severity OA‐induced lesions, histologically...

Aim: to determine if chondrocytic Hsp70 induction, via intra-articular injections of a reversible proteasome inhibitor (MG132), can protect articular chondrocytes from cellular death in experimental rat OA knee induced surgically by anterior cruciate