A5034206799- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Mass Spectrometry Techniques and Applications
- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Biosimilars and Bioanalytical Methods
- Glycosylation and Glycoproteins Research
- Analytical chemistry methods development
- Legume Nitrogen Fixing Symbiosis
University of Michigan
2022-2025
Bispecific antibodies (bsAbs) represent a critically important class of emerging therapeutics capable targeting two different antigens simultaneously. As such, bsAbs have been developed as effective treatment agents for diseases that remain challenging conventional monoclonal antibody (mAb) to access. Despite these advantages, are intricate molecules, requiring both the appropriate engineering and pairing heavy light chains derived from separate parent mAbs. Current analytical tools tracking...
Antibody-based therapeutics have demonstrated remarkable therapeutic benefit, but their susceptibility to biotransformation and degradation in the body can affect safety, efficacy, pharmacokinetic/pharmacodynamic (PK/PD) profiles. In vitro stability assessments play a pivotal role proactively identifying potential liabilities of antibody prior animal studies. Liquid chromatography-mass spectrometry (LC-MS)-based assays has been developed adopted biopharmaceutical industry for...
Fc-fusion proteins are an emerging class of protein therapeutics that combine the properties biological ligands with unique fragment crystallizable (Fc) domain immunoglobulin G (IgG). Due to their diverse higher-order structures (HOSs), remain challenging characterization targets within biopharmaceutical pipelines. While high-resolution biophysical tools available for HOS characterization, they frequently demand extended time frames and substantial quantities purified samples, rendering them...
Abstract Resistant starch is a prebiotic with breakdown by gut bacteria requiring the action of specialized amylases and starch-binding proteins. The human symbiont Ruminococcus bromii expresses granular protein Sas6 (Starch Adherence System member 6) that consists two starch-specific carbohydrate binding modules from family 26 (RbCBM26) 74 (RbCBM74). Here we present crystal structures Rb CBM74 double helical dimer maltodecaose bound along an extended surface groove. Binding data combined...
Bispecific antibodies (bsAbs) represent a critically important class of emerging therapeutics capable simultaneously two different antigens simultaneously. As such, bsAbs have been developed as effective treatment agents for diseases that remain challenging conventional monoclonal antibody (mAb) to access. Despite this, are intricate molecules, requiring both the appropriate engineering and pairing linked heavy light chains derived from separate parent mAbs. Current analytical tools tracking...