A5085344332- Sirtuins and Resveratrol in Medicine
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- MicroRNA in disease regulation
- Biochemical effects in animals
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Circular RNAs in diseases
- Tryptophan and brain disorders
- Gene expression and cancer classification
- Extracellular vesicles in disease
- Advanced biosensing and bioanalysis techniques
- Autophagy in Disease and Therapy
- SARS-CoV-2 detection and testing
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Single-cell and spatial transcriptomics
- Cytokine Signaling Pathways and Interactions
- NF-κB Signaling Pathways
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Retinal Development and Disorders
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
Ben-Gurion University of the Negev
2021-2024
National Institute of Genomic Medicine
2016-2021
Instituto Politécnico Nacional
2021
Centro de Investigación del Cáncer
2017
As to date, more than 49 million confirmed cases of Coronavirus Disease 19 (COVID-19) have been reported worldwide. Current diagnostic protocols use qRT-PCR for viral RNA detection, which is expensive and requires sophisticated equipment, trained personnel previous extraction. For this reason, we need a faster, direct versatile detection method better epidemiological management the COVID-19 outbreak. In work, propose without extraction, based on Loop-mediated isothermal amplification (LAMP)...
Several neurodegenerative diseases present Tau accumulation as the main pathological marker. post-translational modifications such phosphorylation and acetylation are increased in neurodegeneration. Here, we show that hyper-acetylation at residue 174 increases its own nuclear presence is result of DNA damage signaling or lack SIRT6, both causative Tau-K174ac deacetylated nucleus by SIRT6. However, SIRT6 chronic results accumulation. Once there, it induces global changes gene expression,...
Brain-specific SIRT6-KO mice present increased DNA damage, learning impairments, and neurodegenerative phenotypes, placing SIRT6 as a key protein in preventing neurodegeneration. In the aging brain, levels/activity decline, which is accentuated Alzheimer's patients. To understand roles transcript pattern changes, we analyzed transcriptomes of young WT, old WT brains, found changes gene expression related to healthy pathological aging. addition, traced these differences human mouse samples...
Multicellular tumor spheroids (MCTSs) constitute a three-dimensional culture system that recapitulates the in vivo microenvironment. Tumor cells cultured as MCTSs present antineoplastic resistance due to effect of microenvironmental signals acting upon them. In this work, we evaluated biological function new microenvironment-regulated long non-coding RNA, lncMat2B, breast cancer. MCTSs, expression lncMat2B presented an increase and zonal heterogeneity, it was expressed principally quiescent...
During an organism’s lifespan, two main phenomena are critical for the survival. These (1) a proper embryonic development, which permits new organism to function with high fitness, grow and reproduce, (2) aging process, will progressively undermine its competence fitness survival, leading death. Interestingly these processes present various similarities at molecular level. Notably, as organisms became more complex, regulation of coordinated by brain, failure in brain activity is detrimental...
Abstract Oxygen or nutrient deprivation of early stage tumoral spheroids can be used to reliably mimic the initial growth primary and metastatic cancer cells. However, cell during stages has not been fully explored using a genome-wide approach. Thus, in present study, we investigated transcriptome breast cells RNAseq model Multicellular Tumor Spheroids (MTS). Network analyses showed that signature was enriched as several adhesion molecules were deregulated, including EPCAM, E-cadherin,...
Breast cancer is a leading cause of cancer-related deaths among women. Cisplatin used for treatment, but the development resistance in cells significant concern. This study aimed to investigate changes transcriptomes cisplatin-resistant MCF7 cells. We conducted RNA sequencing cells, followed by differential expression analysis and bioinformatic investigations identify gene modified signal transduction pathways. examined size quantity extracellular vesicles. A total 724 genes exhibited...
Retinal Müller glial cells (MGs) are among the first to demonstrate metabolic changes during retinal disease and a potential source of regenerative cells. In response harmful stimulus, they can dedifferentiate acquiring neural stem properties, proliferate migrate damaged layer differentiate into lost neurons. However, it is not yet known how this reprogramming process regulated in mammals. Since glucose oxygen important regulatory elements that may help directing cell fate, we aimed study...
<title>Abstract</title> In the brain, tryptophan byproducts are involved in biosynthesis of proteins, energy-rich molecules (e.g., NAD<sup>+</sup>), and neurotransmitters (serotonin melatonin). Impaired catabolism, seen aging, neurodegeneration psychiatric diseases affects mood, learning, sleep; however, reasons for those impairments remain unknown. Our results from cellular, <italic>drosophila melanogaster</italic>, mouse models indicate that SIRT6 regulates catabolism by balancing its...
RE-1 silencing transcription factor (REST) is a key repressor of neural genes. REST upregulated under stress signals, aging and neurodegenerative diseases, but although it upregulated, its function lost in Alzheimer's Disease. However, why becomes inactive remains unclear. Here, we show that the NAD-dependent deacetylase SIRT6 regulates expression, location activity. In absence SIRT6, overexpressed mislocalized, leading to partial loss activity causing become toxic. deficiency abrogates EZH2...
Long-non-coding RNAs, a class of transcripts with lengths > 200 nt, play key roles in tumour progression. Previous reports revealed that LINC00052 (long intergenic non-coding RNA 00052) was strongly downregulated during breast cancer multicellular spheroids formation and suggested role cell migration oxidative metabolism.To examine the function MCF-7 cells.Loss-of-function studies were performed to evaluate on cells. Microarray expression assays determine genes cellular functions modified...
<title>Abstract</title> <italic>RE-1 Silencing Transcription factor (REST)</italic> <italic>is a key repressor of neural genes. REST is upregulated under stress signals, aging and neurodegenerative diseases, but although it upregulated, loses its function in Alzheimer's Disease. However, why becomes inactive remains unclear. Here, we show that the NAD-dependent deacetylase SIRT6 regulates expression, location activity. In absence, overexpressed mislocalized, part activity, becoming toxic....
En este artículo se presenta un nuevo uso para la tecnología de edición genómica crispr-Cas, cuyo desarrollo le permitió ganar el premio Nobel Química 2020 a las doctoras Jennifer Doudna y Emmanuelle Charpentier. Esta herramienta no sólo permite modificar secuencias adn, sino que también puede utilizar como una prueba muy rápida sensible diagnóstico clínico. Debido reciente pandemia, ha generado crecimiento exponencial métodos detectar sars-CoV-2, causante covid-19, entre los destaca...