A5089940050- Neuroscience and Neuropharmacology Research
- Genetic Neurodegenerative Diseases
- SARS-CoV-2 and COVID-19 Research
- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Monoclonal and Polyclonal Antibodies Research
- Receptor Mechanisms and Signaling
- Mitochondrial Function and Pathology
- Protein purification and stability
- Viral gastroenteritis research and epidemiology
- Stress Responses and Cortisol
- SARS-CoV-2 detection and testing
- Photoreceptor and optogenetics research
- Sleep and Wakefulness Research
- Biochemical Analysis and Sensing Techniques
- Neuroscience, Education and Cognitive Function
- Virus-based gene therapy research
- Cardiac, Anesthesia and Surgical Outcomes
- COVID-19 Clinical Research Studies
- Anesthesia and Neurotoxicity Research
- Neuroendocrine regulation and behavior
- Neuropeptides and Animal Physiology
- Circadian rhythm and melatonin
- Regulation of Appetite and Obesity
- Anesthesia and Sedative Agents
Charité - Universitätsmedizin Berlin
2013-2023
Humboldt-Universität zu Berlin
2020-2023
Freie Universität Berlin
2020-2023
University of Bristol
2016-2017
University College London
2012
The locus coeruleus (LC) projects throughout the brain and spinal cord is major source of central noradrenaline. It remains unclear whether LC acts functionally as a single global effector or discrete modules. Specifically, while spinal-projections from neurons can exert analgesic actions, it not known they act independently ascending projections. Using viral vectors taken up at axon terminals, we expressed chemogenetic actuators selectively in with (LC:SC) prefrontal cortex (LC:PFC)...
Noradrenergic neurons of the brainstem extend projections throughout neuraxis to modulate a wide range processes including attention, arousal, autonomic control and sensory processing. A spinal projection from locus coeruleus (LC) is thought regulate nociceptive To characterize selectively manipulate pontospinal noradrenergic in rats, we implemented retrograde targeting strategy using canine adenoviral vector express channelrhodopsin2 (CAV2-PRS-ChR2-mCherry). LC microinjection...
Opioids are widely used medicinally as analgesics and abused for hedonic effects, actions that each complicated by substantial risks such cardiorespiratory depression. These drugs mimic peptides β-endorphin, which has a key role in endogenous analgesia. The β-endorphin the central nervous system originates from pro-opiomelanocortin (POMC) neurons arcuate nucleus of solitary tract (NTS). Relatively little is known about NTSPOMC but their position within sensory vagus led us to test hypothesis...
Sensory circuits are shaped by experience in early postnatal life and many brain areas late maturation of inhibition drives activity-dependent development. In the newborn spinal dorsal horn, activity is dominated inputs from low threshold A fibers, whereas nociceptive C-fiber mature gradually over first weeks. How this changing afferent input influences horn not known. We show an absence functional glycinergic circuits: Dorsal receptive fields afferent-evoked excitation initially facilitated...
The rapid evolution of new SARS-CoV-2 variants poses a continuing threat to human health. Vaccination has become the primary therapeutic intervention. goal current work was construction immunogenic virus-like particles (VLPs). Here, we describe cell line for cost-efficient and scalable production VLPs. modular design VLP-production platform facilitates adaptation variants. Methods: N, M-, E-protein genes were integrated into genome Expi293 cells (ExpiVLP_MEN). Subsequently, this further...
Rapid removal of glutamate from the sites release is an essential step in excitatory synaptic transmission. However, despite many years research, molecular mechanisms underlying intracellular regulation transport at tripartite synapses have not been fully uncovered. This limits options for pharmacological treatment glutamate-related motor disorders, including Huntington's disease (HD). We therefore investigated possible binding partners transgenic EAAT2 and their alterations under influence...
The ongoing COVID-19 pandemic situation caused by SARS-CoV-2 and variants of concern such as B.1.617.2 (Delta) recently, B.1.1.529 (Omicron) is posing multiple challenges to humanity. rapid evolution the virus requires adaptation diagnostic therapeutic applications.In this study, we describe camelid heavy-chain-only antibodies (hcAb) useful tools for novel in vitro assays applications due their neutralizing capacity.Five antibody candidates were selected out a naïve library phage display...
A key in controlling the SARS-CoV-2 pandemic is assessment of immune status population. We explored utility virus-like particles (VLPs) as antigens to detect specific humoral reactions an enzyme-linked immunosorbent assay (ELISA). For this purpose, VLPs were produced from engineered cell line and characterized by Western blot, ELISA, nanoparticle tracking analysis. Subsequently, we collected 42 serum samples before (2014), 89 healthy subjects, 38 vaccinated subjects. Seventeen less than...
SUMMARY Rapid removal of glutamate from the sites release is an essential step in excitatory synaptic transmission. Despite many years research, molecular mechanisms underlying intracellular regulation transport at tripartite synapses have remained unclear. This limits options for pharmacological treatment motor disorders associated with excitotoxicity. Therefore, using Q175 mouse model Huntington’s disease (HD), we explored effects structural changes astrocytic amino acid transporter type 2...
Deficiency of the astrocytic excitatory amino acid transporter type 2 (EAAT2) and, consequently, inefficient glutamate clearance at corticostriatal synapses may contribute to depression self-initiated movements in Huntington’s disease (HD). Here we report that removal last 68 amino-acids C-terminal EAAT2 increases levels native striatal lysates and counteracts some HD-related changes interactor spectrum mYFP-tagged EAAT2. Using Q175 mouse model HD, explored functional consequences...
ABSTRACT The locus coeruleus (LC) projects throughout the brain and spinal cord is major source of central noradrenaline. It remains unclear whether LC acts functionally as a single global effector or discrete modules. Specifically, while spinal-projections from neurons can exert analgesic actions, it not known they act independently ascending projections. Using viral vectors taken up at axon terminals, we expressed chemogenetic actuators selectively in with ( :SC ) prefrontal cortex :PFC...
Abstract Introduction The ongoing COVID-19 pandemic situation caused by SARS-CoV-2 and variants of concern such as B.1.617.2 (Delta) recently, B.1.1.529 (Omicron) is posing multiple challenges to humanity. rapid evolution the virus requires adaptation diagnostic therapeutic applications. Objectives In this study, we describe camelid heavy-chain-only antibodies (hcAb) useful for novel in vitro assays applications due their neutralizing capacity. Methods Five antibody candidates were selected...