A5009442781- Cystic Fibrosis Research Advances
- Infections and bacterial resistance
- Plant Pathogenic Bacteria Studies
- Genomics and Phylogenetic Studies
- Respiratory viral infections research
- Legume Nitrogen Fixing Symbiosis
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Salmonella and Campylobacter epidemiology
- Plant Disease Resistance and Genetics
- Actinomycetales infections and treatment
- Antibiotic Resistance in Bacteria
- Entomopathogenic Microorganisms in Pest Control
- Plant-Microbe Interactions and Immunity
- Gut microbiota and health
- Iron Metabolism and Disorders
- Geriatric Care and Nursing Homes
- Infective Endocarditis Diagnosis and Management
- COVID-19 Clinical Research Studies
- Infectious Encephalopathies and Encephalitis
- COVID-19 and Mental Health
- Microbial Community Ecology and Physiology
Rega Institute for Medical Research
2024
KU Leuven
2024
AZ Sint-Lucas
2015-2023
Ghent University
2003-2009
Hacettepe University
2006
King's College Hospital
2006
The aim of the present study was to re-examine taxonomic position and structure taxon K (also known as group K) within Burkholderia cepacia complex (Bcc). For this purpose, a representative set strains examined by traditional polyphasic approach, multilocus sequence typing (MLST) analysis available whole-genome sequences. Analysis recA gene revealed three different lineages, designated recA-I, recA-II recA-III. DNA-DNA hybridization experiments demonstrated that recA-I isolates each...
The taxonomic position of five recA gene clusters Burkholderia cepacia complex (Bcc) isolates was determined using a polyphasic approach. levels 16S rRNA and sequence similarity, multilocus typing (MLST) data the intermediate DNA–DNA binding values demonstrated that these represented novel species within Bcc. Biochemical identification is difficult, as case for most Bcc species. However, can be accomplished through analysis, MLST BOX-PCR profiling by RFLP analysis. For diagnostic...
To evaluate the feasibility of different methods for susceptibility testing human Arcobacter isolates, to assess antibiotics commonly used treat diarrhoeal illness and obtain MIC distribution data. One-hundred-and-six unique strains were collected during an epidemiological study on pathogens in gastroenteritis. Strains identified by multiplex PCR PCR–RFLP, characterized PFGE. Susceptibility ampicillin, erythromycin, tetracycline, doxycycline, gentamicin ciprofloxacin was determined using...
From early 2020, a high demand for SARS-CoV-2 tests was driven by several testing indications, including asymptomatic cases, resulting in the massive roll-out of PCR assays to combat pandemic. Considering dynamic viral shedding during course infection, report cycle threshold (Ct) values rapidly emerged. As Ct can be affected number factors, we considered that harmonization semi-quantitative results across laboratories would avoid potential divergent interpretations, particularly absence...
Shot-gun sequencing of DNA isolated from the environment and assembly metagenomes resulting data has considerably advanced study microbial diversity. However, subsequent matching these hypothetical to cultivable microorganisms is a limitation such cultivation-independent methods population analysis. Using nucleotide sequence-based genetic typing method, multilocus sequence typing, we were able for first time match clonal isolates published controversial bacterial metagenome, Burkholderia...
A polyphasic taxonomic study of 14 isolates recovered from various human and veterinary clinical samples was performed. Phenotypically these shared several characteristics with members the Alcaligenaceae related genera. Random amplified polymorphic DNA fingerprinting whole-cell protein analysis suggested presence multiple genomic groups, which confirmed by DNA–DNA hybridization experiments. 16S rRNA gene sequence indicated that were to genera Pelistega , Taylorella Oligella Pigmentiphaga...
Type and reference strains of Stenotrophomonas maltophilia africana were compared with each other the type species, using SDS-PAGE whole-cell proteins, DNA-DNA hybridization extensive biochemical characterization. S. LMG 958T 22072T had very similar whole-cell-protein patterns also biochemically similar. A binding level 70% between both confirmed that represent same taxon. It is concluded a later synonym maltophilia.
A polyphasic taxonomic study was performed to compare 26 novel bacterial isolates obtained from (semi-)natural grassland soils and a heathland soil in the Netherlands with 16 strains that had previously been assigned genus Collimonas. Genomic fingerprinting (BOX-PCR), whole-cell protein electrophoresis, matrix-assisted laser desorption ionization time-of-flight mass spectrometry of intact cells physiological characterization (Biolog) confirmed existence different strain clusters (A-D) within...
A Gram-negative, rod-shaped, aerobic bacterium, designated strain RP007(T), was isolated from a polycyclic aromatic hydrocarbon-contaminated soil in New Zealand. Two additional strains were recovered compost heap Belgium (LMG 18808) and the rhizosphere of maize Netherlands 24204). The three had virtually identical 16S rRNA gene sequences whole-cell protein profiles, they identified as members genus Burkholderia, with Burkholderia phenazinium their closest relative. Strain RP007(T) DNA G+C...
Stenotrophomonas maltophilia isolates have been recovered from various clinical samples, including the respiratory tract of cystic fibrosis (CF) patients, but this organism is also widespread in nature. Previously it has shown that there a considerable genomic diversity within S. maltophilia. The aims our study were to determine taxonomic resolution restriction fragment length polymorphism (RFLP) analysis polymerase chain reaction-amplified gyrB gene for genus Stenotrophomonas. Subsequently,...
The purpose of this study was to develop a novel strategy for the isolation and identification Burkholderia cepacia complex bacteria from home environment cystic fibrosis (CF) patients. Water soil samples were enriched in broth containing 0.1% l-arabinose, l-threonine, mixture selective agents including 1 microgml(-1) C-390, 600U ml(-1) polymyxin B sulfate, 10 gentamycin, 2 vancomycin cycloheximide. On media (consisting same components as above plus 1.8% agar), several dilutions enrichment...
Introduction. The FilmArray Meningitis/Encephalitis (FA-ME) Panel (Biofire, Salt Lake City, Utah, US) enables fast and automated detection of 14 pathogens in cerebrospinal fluid (CSF). Gap statement. performance the FA-ME panel a real routine setting has not yet been described could lead to better patient management cases good performance. Aim. This multicenter study verified analytical hospital setting. Methodology. Between April 2016 2018, 454 CSF samples were analysed with compared...