A5044985536- Advanced Chemical Sensor Technologies
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Bacterial Identification and Susceptibility Testing
- Biosensors and Analytical Detection
- Forensic Toxicology and Drug Analysis
- Escherichia coli research studies
- Analytical Chemistry and Chromatography
- Salmonella and Campylobacter epidemiology
- Chemical and Physical Properties in Aqueous Solutions
- Research Data Management Practices
- Mollusks and Parasites Studies
- Listeria monocytogenes in Food Safety
- Helminth infection and control
- Immune Response and Inflammation
- Meat and Animal Product Quality
- Immunotherapy and Immune Responses
- Antimicrobial Peptides and Activities
- Parasite Biology and Host Interactions
- Antibiotic Resistance in Bacteria
- Microbial Metabolic Engineering and Bioproduction
- Vibrio bacteria research studies
- Evolutionary Algorithms and Applications
- Privacy-Preserving Technologies in Data
- Data Quality and Management
University Children’s Hospital Basel
2022-2024
University of Basel
2022-2024
University of Zurich
2017-2018
University of Wisconsin–Madison
1995-2014
Salisbury University
2010
WiCell
1996
Stationary phase and the starvation of log-phase cells increased acid tolerance Escherichia coli O157:H7 strains. Although degree varied, survival most strains exceeded that other, related, pathogens in a synthetic gastric fluid.
A survey was conducted between March and October of 1994 to determine the prevalence identify sources serotype O157:H7 isolates Escherichia coli in Wisconsin dairy herds. stratified sample 400 farms identified, 70 with weaned calves less than 4 months old were included study. During study, 5 (herd prevalence, 7.1 +/- 4.5%) fecal samples from 10 560 (animal 1.8%) tested positive for O157:H7. In a follow-up five O157:H7-positive seven O157:H7-negative identified study visited again. An...
An rpoS mutant (rpoS::pRR10) of Escherichia coli O157:H7 ATCC 43895 was generated. Stationary-phase acid, heat, and salt tolerance significantly reduced, starvation-induced acid did not develop in the mutant. RpoS also important for survival E. dry, fermented sausage.
Early detection of pathogenic bacteria is needed for rapid diagnostics allowing adequate and timely treatment infections. In this study, we show that secondary electrospray ionization-high resolution mass spectrometry (SESI-HRMS) can be used as a diagnostic tool bacterial infections supportive system current state-of-the-art diagnostics. Volatile organic compounds (VOCs) produced by growing S. aureus or pneumoniae cultures on blood agar plates were detected within minutes allowed the...
Abstract Urine adulteration to circumvent positive drug testing is a fundamental challenge for toxicological laboratories all over the world. Untargeted mass spectrometry (MS) methods used in metabolomics had previously revealed uric acid (UA), histidine, methylhistidine, and their oxidation products, example 5‐hydroxyisourate (HIU) as potential biomarkers urine using potassium nitrite (KNO 2 ). These markers should be further evaluated reliability, stability, routine applicability....
Dendritic cells (DCs) actively sample and present antigen to of the adaptive immune system are thus vital for successful control memory formation. Immune cell metabolism function tightly interlinked, a better understanding this interaction offers potential develop immunomodulatory strategies. However, current approaches assessing metabolome often limited by end-point measurements, may involve laborious preparation, lack unbiased, temporal resolution metabolome. In study, we novel setup...
An adult, female, free-ranging, sika deer (Cervus nippon yakushimae) from Wicomico County, Maryland, USA, was found circling and having no fear of humans. The animal euthanized submitted for a postmortem exam. There were gross lesions the negative rabies. Microscopic examination revealed lymphoplasmacytic, neutrophilic, eosinophilic meningoencephalitis with intralesional adult nematodes, larvae, eggs consistent nematodes in family Protostrongylidae. Parelaphostrongylus tenuis identified by...
Abstract Secondary electrospray ionization-high resolution mass spectrometry (SESI-HRMS) is an established technique in the field of breath analysis characterized by its short time, as well high levels sensitivity and selectivity. Traditionally, SESI-HRMS has been used for real-time analysis, which requires subjects to be at location analytical platform. Therefore, it limits possibilities introduction this methodology day-to-day clinical practice. However, recent methodological developments...
Abstract Introduction The ability to detect pathogenic bacteria before the onsets of severe respiratory symptoms and differentiate bacterial infection allows improve patient-tailored treatment leading a significant reduction in illness severity, comorbidity as well antibiotic resistance. As such, this study refines application non-invasive Secondary Electrospray Ionization-High Resolution Mass Spectrometry (SESI-HRMS) methodology for real-time early detection human pathogens tract mouse...
Objective: The aim of this work was to explore the feasibility in vivo and non-invasive monitoring deuterium/hydrogen ( 2 H/ 1 H) exchange at metabolic level upon exposure heavy water H O). Methods: healthy female mice were randomly assigned two groups after day 0 when both received standard drinking water. treated mouse fed with O (80%, v/v) control (H O) over next 13 days. Real-time mass spectrometric analysis volatile metabolism emitted through breathing skin performed on days 1, 2, 3,...
ABSTRACT Rapid detection of pathogenic bacteria is needed for rapid diagnostics allowing adequate and timely treatment. In this study, we aimed to evaluate the technical feasibility Secondary Electro-Spray Ionization-High Resolution Mass Spectrometry (SESI-HRMS) as a diagnostic tool bacterial infections compare its performance with current standard diagnostics. We compared time required confirm growth Staphylococcus aureus Streptococcus pneumoniae by conventional culture MAL-DI-TOF vs....
Early detection of pathogenic bacteria is needed for rapid diagnostics allowing adequate and timely treatment infections. In this study, we show that Secondary Electro-Spray Ionization-High Resolution Mass Spectrometry (SESI-HRMS) can be used as a diagnostic tool bacterial infections supportive system current state the art diagnostics. Volatile Organic Compounds (VOCs) produced by growing S. aureus or pneumoniae cultures on blood agar plates were detected within minutes allowed distinction...