A5010556512- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Lung Cancer Treatments and Mutations
- Metabolism, Diabetes, and Cancer
- Muscle Physiology and Disorders
- Hippo pathway signaling and YAP/TAZ
- ATP Synthase and ATPases Research
- Microtubule and mitosis dynamics
- Genetic factors in colorectal cancer
- Congenital heart defects research
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Neonatal Respiratory Health Research
- Ubiquitin and proteasome pathways
- DNA and Nucleic Acid Chemistry
- Signaling Pathways in Disease
- Mitochondrial Function and Pathology
- Angiogenesis and VEGF in Cancer
University of Florida
2015-2024
Florida College
2010-2024
University of Florida Health
2013-2024
Nanjing Drum Tower Hospital
2016-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Harbin Medical University
2024
Shaanxi Provincial People's Hospital
2022-2023
Nanyang Normal University
2022
UF Health Cancer Center
2018-2021
Institute of Genetics
2021
Myocyte enhancer factor-2 (MEF2) transcription factors control muscle-specific and growth factor-inducible genes. We show that hypertrophic of cardiomyocytes in response to phenylephrine serum is accompanied by activation MEF2 through a posttranslational mechanism mediated calcium, calmodulin-dependent protein kinase (CaMK), mitogen-activated (MAPK) signaling. CaMK stimulates activity dissociating class II histone deacetylases (HDACs) from the DNA-binding domain. MAPKs, which activate...
Paclitaxel (Taxol) is an effective chemotherapeutic agent for treatment of cancer patients. Despite impressive initial clinical responses, the majority patients eventually develop some degree resistance to Taxol-based therapy. The mechanisms underlying cells Taxol are not fully understood. MicroRNA (miRNA) has emerged play important roles in tumorigenesis and drug resistance. However, interaction between development miRNA been previously explored. In this study we utilized a array compare...
Abstract Background Taxol is one of the most effective chemotherapeutic agents for treatment patients with breast cancer. Despite impressive clinical responses initially, majority eventually develop resistance to Taxol. Lactate dehydrogenase-A (LDH-A) predominant isoforms LDH expressed in tissue, which controls conversion pyruvate lactate and plays an important role glucose metabolism. In this study we investigated LDH-A mediating human cancer cells. Results Taxol-resistant subclones,...
The LKB1 tumor suppressor gene is frequently mutated and inactivated in non-small cell lung cancer (NSCLC). Loss of promotes progression influences therapeutic responses preclinical studies; however, specific targeted therapies for with inactivation are currently unavailable. Here, we have identified a long noncoding RNA (lncRNA) signature that associated the loss function. We discovered LINC00473 consistently most highly induced LKB1-inactivated human primary NSCLC samples derived lines....
ABSTRACT The embryonic vasculature develops from endothelial cells that form a primitive vascular plexus which recruits smooth muscle to the arterial and venous systems. MADS-box transcription factor MEF2C is expressed in developing (SMCs), as well surrounding mesenchyme, during embryogenesis. Targeted deletion of mouse gene resulted severe abnormalities lethality homozygous mutants by day 9.5. Endothelial were present able differentiate, but failed organize normally into plexus, did not...
PICK1 is a protein kinase C (PKC) α-binding initially identified using the yeast two-hybrid system. Here we report that contains PDZ domain interacts specifically with previously unidentified PDZ-binding (QSAV) at extreme COOH terminus of PKCα and mutation putative carboxylate-binding loop within abolishes this interaction. The in absent from other PKC isoforms do not interact PICK1. We also demonstrate can homooligomerize through sequences are distinct loop, suggesting self-association...
Members of the GATA family zinc finger transcription factors have been shown to play important roles in control gene expression a variety cell types. GATA-1, -2, and -3 are expressed primarily hematopoietic lineages required for proliferation differentiation multiple types, whereas GATA-4, -5, -6 heart, where they activate cardiac muscle structural genes. Friend GATA-1 (FOG) is multitype protein that interacts with serves as cofactor GATA-1-mediated transcription. FOG coexpressed developing...
The class II histone deacetylases (HDACs) 4, 5, and 7 share a common structural organization, with carboxyl-terminal catalytic domain an amino-terminal extension that mediates interactions members of the myocyte enhancer factor-2 (MEF2) family transcription factors. Association these HDACs MEF2 factors represses target genes. MEF2-interacting repressor (MITR) shares homology extensions also acts as transcriptional repressor, but lacks deacetylase domain. This suggests MITR by recruiting...
Histone deacetylase 6 (HDAC6) is a cytoplasmic that uniquely catalyzes alpha-tubulin deacetylation and promotes cell motility. However, the mechanism underlying HDAC6-dependent migration role for microtubule acetylation in motility are not known. Here we show HDAC6-induced global was sufficient to stimulate migration. Unexpectedly, response growth factor stimulation, HDAC6 underwent rapid translocation actin-enriched membrane ruffles subsequently became associated with macropinosomes,...
Cancer cells exhibit altered glucose metabolism characterized by a preference for aerobic glycolysis or the Warburg effect, and resist matrix detachment-induced apoptosis, which is called anoikis, barrier to metastasis. It remains largely unclear whether tumor influences anoikis Here we show that when detached from matrix, untransformed mammary epithelial undergo metabolic reprogramming markedly upregulating pyruvate dehydrogenase (PDH) kinase 4 (PDK4) through estrogen-related receptor gamma...
Members of the MyoD family basic helix-loop-helix (bHLH) transcription factors control formation all skeletal muscles in vertebrates, but little is known molecules or mechanisms that confer unique identities to different types muscles. MyoR and capsulin are related bHLH expressed specific facial muscle precursors. We show missing mice lacking both , reflecting absence gene expression ablation corresponding myogenic lineages. These findings identify as for development head
Normal cells require adhesion to extracellular matrix for survival. Cell detachment causes a drastic increase in reactive oxygen species (ROS) that promotes anoikis. In the present study, we observed upon from matrix, human mammary epithelial strongly upregulate manganese superoxide dismutase (MnSOD, or SOD2), principal mitochondrial antioxidant enzyme detoxifies ROS through dismutation of superoxide. Induction MnSOD by cell is dependent on NFκB transcription factor. Detachment potently...