A5046935079- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Advanced MRI Techniques and Applications
- Nanoplatforms for cancer theranostics
- Mass Spectrometry Techniques and Applications
- Medical Imaging Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cancer Research and Treatments
- MRI in cancer diagnosis
- Cancer, Lipids, and Metabolism
- Advanced Proteomics Techniques and Applications
- Lanthanide and Transition Metal Complexes
- Redox biology and oxidative stress
- Advanced Fluorescence Microscopy Techniques
- Retinoids in leukemia and cellular processes
- Photodynamic Therapy Research Studies
- Cancer Cells and Metastasis
- Photoacoustic and Ultrasonic Imaging
- Advanced NMR Techniques and Applications
- Optical Coherence Tomography Applications
- Glycosylation and Glycoproteins Research
- Ion-surface interactions and analysis
- Radiomics and Machine Learning in Medical Imaging
- RNA modifications and cancer
- Angiogenesis and VEGF in Cancer
Johns Hopkins University
2016-2025
Sidney Kimmel Comprehensive Cancer Center
2016-2025
Johns Hopkins Medicine
2015-2024
Imaging Center
2009-2024
University of Baltimore
2010-2018
Second Hospital of Shandong University
2017
Morgan State University
2012-2016
Johns Hopkins Hospital
2015
Corning (United States)
2012
National Cancer Institute
2012
Abstract Proton magnetic resonance spectroscopy (1H MRS) consistently detects significant differences in choline phospholipid metabolites of malignant versus benign breast lesions. It is critically important to understand the molecular causes underlying these metabolic differences, because this may identify novel targets for attack cancer cells. In study, membrane metabolism were characterized cells and normal human mammary epithelial (HMECs) labeled with [1,2-13C]choline, using 1H 13C...
Cancer cells invade by secreting degradative enzymes, which are sequestered in lysosomal vesicles. In this study, the impact of an acidic extracellular environment on lysosome size, number, and distance from nucleus human mammary epithelial (HMECs) breast cancer different degrees malignancy was characterized because physiological microenvironment tumors is frequently acidity. An pH (pH(e)) resulted a distinct shift lysosomes perinuclear region to cell periphery irrespective HMECs' degree...
Altered phosphatidylcholine (PC) metabolism in epithelial ovarian cancer (EOC) could provide choline-based imaging approaches as powerful tools to improve diagnosis and identify new therapeutic targets. The increase the major choline-containing metabolite phosphocholine (PCho) EOC compared with normal nontumoral immortalized counterparts (EONT) may derive from (a) enhanced choline transport kinase (ChoK)-mediated phosphorylation, (b) increased PC-specific phospholipase C (PC-plc) activity,...
Abstract Choline kinase is overexpressed in breast cancer cells and activated by oncogenes mitogenic signals, making it a potential target for therapy. Here, we have examined, the first time, effects of RNA interference (RNAi)–mediated down-regulation choline nonmalignant malignant human epithelial cell lines using magnetic resonance spectroscopy (MRS) as well molecular analyses proliferation differentiation markers. RNAi knockdown reduced proliferation, detected proliferating nuclear...
The lipid compositions of different breast tumor microenvironments are largely unknown due to limitations in imaging techniques. Imaging distributions would enhance our understanding processes occurring inside growing tumors, such as cancer cell proliferation, invasion, and metastasis. Recent developments MALDI mass spectrometry (MSI) enable rapid specific detection lipids directly from thin tissue sections. In this study, we performed multimodal acylcarnitines, phosphatidylcholines (PC), a...
Collagen I (Col1) fibers are a major structural component in the extracellular matrix of human breast cancers. In preliminary pilot study, we explored link between Col1 fiber density primary cancers and occurrence lymph node metastasis. were detected by second harmonic generation (SHG) microscopy from patients presenting with metastasis (LN+) versus those without (LN−). density, which was quantified using our in-house SHG image analysis software, significantly higher LN+ (fiber...
Abstract Proline is a readily released stress substrate that can be metabolized by proline oxidase (POX) to generate either reactive oxygen species (ROS) induce apoptosis or autophagy ATP during times of nutrient stress. However, the contribution metabolism tumorigenesis in hypoxic microenvironments has not been explored. In this study, we investigated different functions POX under hypoxia and glucose depletion. We found induced expression cancer cells vitro upregulation colocalized with...
BACKGROUND. Although aberrant glycosylation is recognized as a hallmark of cancer, in clinical breast cancer (BC) metastasis has not yet been studied. While preclinical studies show that the glycocalyx coating cells involved adhesion, migration, and metastasis, changes from primary tumor (PT) to various metastatic sites remain unknown patients.
The molecular pathology of breast cancer is challenging due to the complex heterogeneity cellular subtypes. ability directly identify and visualize cell subtype distribution at single-cell level within a tissue section enables precise rapid diagnosis prognosis. Here, we applied mass spectrometry imaging (MSI) acquire profiles subcellular levels 14 different lines. We built library genetically well-characterized Multistep processing, including deep learning, resulted in subtype, cancer's...
Abstract The intensity of the total choline (tCho) signal in spectroscopic images tumors is spatially heterogeneous. likewise heterogeneous physiologic tumor microenvironment may contribute to this heterogeneity. We therefore investigated relationship between hypoxia, metabolites, and kinase (Chk) a human prostate cancer model. Human PC-3 cells were engineered express enhanced green fluorescent protein (EGFP) under hypoxic conditions. These PC-3-5HRE-EGFP characterized culture as...
Imaging mass spectrometry (IMS) is a promising technology which allows for detailed analysis of spatial distributions (bio)molecules in organic samples. In many current applications, IMS relies heavily on (semi)automated exploratory data procedures to decompose the into characteristic component spectra and corresponding abundance maps, visualizing spectral structure. The most commonly used techniques are principal (PCA) independent (ICA). Both methods operate an unsupervised manner. However,...
Theranostic imaging, where diagnosis is combined with therapy, particularly suitable for a disease that as complex cancer, especially now genomic and proteomic profiling can provide an extensive "fingerprint" of each tumor. With such information, theranostic agents be designed to personalize treatment minimize damage normal tissue. Here we have developed nanoplex platform imaging prostate cancer (PCa). In these proof-of-principle studies, therapeutic containing multimodal reporters was...
We show on imaging mass spectrometry (IMS) data that the Random Forest classifier can be used for automated tissue classification and it results in predictions with high sensitivities positive predictive values, even when intersample variability is present data. further demonstrate how Markov Fields vector-valued median filtering applied to reduce noise effects improve a posthoc smoothing step. Our study gives clear evidence digital staining by means of IMS constitutes promising complement...