Although ice-free areas cover only about 0.1% of Antarctica and are characterized by harsh environmental conditions, these regions provide quite diverse conditions for the soil-forming process, having various physical geochemical properties, also assuring different living organisms. This study is aimed to determine existing soil microbial communities, their relationship with parameters influence anthropogenic activity in Larsemann Hills, Eastern Antarctica. The microbiome was investigated at...

The rhizosphere community represents an "ecological interface" between plant and soil, providing the with a number of advantages. Despite close connection mutual influence in this system, knowledge about diversity is still controversial. One most valuable factors uncertainty rough estimation diversity. NGS sequencing can make estimations more precise than classical geobotanical methods. We investigate fallow crop sites, which are similar terms environmental conditions soil legacy, yet at...

Abstract Localization of agriculture with the aim local food support has become a very urgent topic for Yamal region. The most fertile soils this region are sandy textured anthropogenically affected soils. Microbiomes from disturbed Nadym were studied using analysis 16S rRNA metagenomic libraries. It was shown that plant cover is driving force microbiome composition. Forest covered aeolian transfers quarry retaids typical forest following dominant bacterial phyla: Proteobateria,...

The collapse of collective farming in Russia after 1990 led to the abandonment 23% agricultural area. Microbial biomass is a transit pool between fresh and soil organic matter; therefore, structural changes microbial community determine carbon cycle processes caused by self-restoration arable lands abandonment. Here, we assessed influence monthly moisture temperature on bacterial structure abundance Retisols under long-term abandoned cropland. Two periods with pronounced differences...

High-throughput 16S rRNA sequencing was performed to compare the microbiomes inhabiting two contrasting soil types-sod-podzolic and chernozem-and corresponding culturome communities of potentially cellulolytic bacteria cultured on standard Hutchinson media. For each type, soil-specific microorganisms have been identified: for sod-podzolic soil-Acidothermus, Devosia, Phenylobacterium Tumebacillus, chernozem soil-Sphingomonas, Bacillus Blastococcus. The dynamics differences between types bulk...

Microbial biodiversity parameters for tropical rainforests remain poorly understood. Whilst the soil microbiome accounts up to 95% of total diversity microorganisms in terrestrial ecosystems, suspended soils formed by vascular epiphytes remains completely unexplored. Samples ground and were collected Cat Tien National Park, southern Vietnam. DNA extraction sequencing performed, libraries 16s rDNA gene sequences analyzed. Alpha indices highest forest soil. In general, microbiological all...

Restoration of anthropogenically disturbed soils is an urgent problem in modern ecology and soil biology. processes northern environments are especially important, due to the small amounts fertile land low levels natural succession. We analyzed microbiota, which one indicators succession process soil. Samples were obtained from three (self-overgrown reclaimed quarries), two undisturbed (primary secondary forests). Primary Forest had a well-developed profile, pH TOC (total organic carbon)...

Abstract Soil microbiome plays an important role in soil forming process as well functioning. This is especially relevant for initial stages of regeneration after strong anthropogenic impact (i.e., quarrying complexes). The study such areas crucial both understanding the forces driving formation and optimization reclamation techniques. aimed to investigate different ages on heaps limestone mines conditions boreal climate North-West Russia. analysis was performed using high-throughput...

Crop rotation is one of the oldest and most effective methods restoring soil fertility, which declines when same plant grown repeatedly. One reasons for a reduction in fertility accumulation pathogenic unfavorable microbiota. The modern crop schemes (a set species their order rotation) are highly but designed without considering microbiota dynamics. main goal this study was to perform short-term experiment with multiple combinations access microbiological effects rotation. It could be useful...

Technogenic processes and agrodevelopment of the soil cover lead to significant transformations chemical biological properties. New methods microbiology, including next-generation sequencing, allows us investigate microbial composition in detail, taxonomy ecological functions bacteria. This study presents data on taxonomic diversity mature anthropogenically disturbed soils various ecosystems Russia. Natural southern taiga (Leningrad region Novgorod region), northern (Komi republic),...

This work describes the microbial community structure of continuously revegetated chronosequence a former sand quarry, which demonstrates unique example nearly complete soil restoration in less than 100 years. Samples were collected at five time points (0, 3, 30, 70 years and mature soil) from entire set horizons, revealing history pedogenesis. Real-time PCR was applied to quantitatively describe bacterial archaeal communities. High-throughput sequencing V4 variable region 16S rRNA gene used...

This research evaluates the development of microbiomes in primary soils, forming various mining dumps arid terrain Republic Bashkortostan, Russia. A metagenomic analysis communities was performed by sequencing extended gene sequences. The evaluation agro-chemical properties accordance with conventional pedology methods. Inverse voltammetry used to measure heavy metals (lead, cadmium, mercury, zinc, copper, and nickel) arsenic content. In all samples studied, Actinobacteria Proteobacteria...

For the targeted selection of microbial communities that provide cellulose degradation, soil samples containing cellulolytic microorganisms and specific plant residues as a substrate can be used. The details this process have not been studied: in particular, whether use different soils determines varying efficiency communities; these established will specificity, other factors. To answer questions, four with activity (Podzol Chernevaya taiga) substrates (oat straw hemp shives) levels...

The microbiome of initial soils formed at the heaps and bottoms surface sediment quarries in surroundings Yakutsk City(Eastern Siberia,Russia) has been characterized for first time. In Entisols, we detected Alphaproteobacteria (represented mainly by family Rizobiales), Gammaproteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes (mostly Chitinophagales), Deltaproteobacteria, Chloroflexi. lower soil horizons had a more homogenous species diversity taxonomy that was dominated...

Abstract Soil microbiome plays a significant role in the development of soil profile and implementation key biochemical processes. Observed Arctic warming creates environmental risks, since permafrost soils contain huge amount organic matter, which is potential risk to be released form greenhouse gases. This work aimed investigate microbiomes mature tundra anthropogenically affected areas Yamal region using high-throughput sequencing approach. The taxonomic analysis revealed 33 bacterial...

Permafrost soils differ significantly from other because they serve as a huge reservoir for organic carbon accumulated during the Quaternary Period, which is at risk of being released Arctic warms. This study aimed to characterize existing pools, delineate possible mineralization risks soil matter and assess microbial communities in tundra forest–tundra permafrost southern Yamal region Russia. The profile distribution carbon, nitrogen C:N ratio showed non-gradual changes with depth due...

Fires have a strong effect on soil microbiome, and the mechanisms of restoration after fires are currently not well understood. This study describes characteristics microbial communities in Psamment Entisol soils pine forests city Togliatti forest crown surface fires. Geochemistry, respiration community structure via 16S rRNA gene sequencing were studied different horizons. Both resulted variations diversity shifts taxonomic composition. There is tendency to an increase proportion...

Сhanges in the composition of microbial communities under influence root exudation plants (rhizosphere effect) is widely reported scientific literature.A number studies clearly show rhizosphere effect external factors such as soil type, species and plant variety, etc.The aim this work to study type using modern highthroughput sequencing techniques.This has been studied well by foreign counterparts, but on Russian soils crops used domestic agro-industry, carried out for first time.We two...