Abstract Human adenovirus (HAdV) types F40/41 have long been recognized as major viral agents of acute gastroenteritis (AGE) in children. Despite this, studies on HAdV molecular epidemiology are sparse, and their real impact is likely under-estimated. Thus, our goal was to investigate incidence, enteric non-enteric circulation, co-detections with rotavirus norovirus DNA shedding stool samples from inpatients outpatients eleven Brazilian states. During the three-year study, 1012 AGE were...

: Brazil introduced universal antirotavirus vaccination in March 2006. This article reports the results of rotavirus A (RV-A) surveillance from January 2005 to December 2009.: total 6109 fecal samples were collected 18 Brazilian states. RV-A was detected by enzyme immunoassay and/or polyacrylamide gel electrophoresis, and genotyped through seminested reverse transcription-polymerase chain reaction.: 20.3% (n = 1242) samples. Among children less than 2 years old, regardless status, rates...

This 15-year study aimed to determine the role of main viruses responsible for acute infantile gastroenteritis cases in a day care center city Rio de Janeiro, Brazil. From 1994 2008, 539 fecal samples were obtained from 23 outbreaks as well sporadic that occurred this period. The detection Rotavirus group A (RVA), norovirus (NoV) and astrovirus (AstV) was investigated both by classical molecular methods viral detection. RVA detected enzymatic immune assay and/or polyacrylamide gel...

Acute gastroenteritis is a major source of morbidity and mortality among young children in developed developing countries. Human adenoviruses (HAdVs), particular species F, are related to childhood diarrhoea worldwide. This study presents the results obtained during an investigation HAdVs causing acute hospitalized Rio de Janeiro, RJ, Brazil, from April 1996 September 2003, as well with living slums Salvador, BA, October 2001 2003. A total 3060 stool samples was analysed by enzyme...

Abstract Brazil introduced rotavirus vaccination in March 2006. We studied 133 rotavirus-positive fecal samples collected from February 2005 through December 2007. Genotype G2P[4] was found 1.4% of 2005, 44% 2006, and 96% Rotavirus detection rate decreased 38% to 24% 2007 (p = 0.012).

ABSTRACT Fifty-three rotavirus-positive fecal specimens from children with diarrhea admitted to a Rio de Janeiro, Brazil, children's hospital between January 1997 and December 1998 were characterized for P G types by using reverse transcription-PCR. Genotype P[4]G2 accounted 21% of isolates, while uncommon genotypes P[8]G9, P[6]G9, P[4]G9 13% the isolates.

Acute gastroenteritis norovirus (NoV) in a country of continental dimensions like Brazil has resulted under-reporting the number outbreaks, as well genotypes associated. To demonstrate role NoV outbreaks occurring State Rio Grande do Sul, Southern Brazil, we determined its prevalence, associated, and evaluated clinical epidemiological aspects. investigation was carried out rotavirus group A negative stool samples from 2265 patients 741 that occurred during period eight years (2004–2011)....

Brazil introduced the monovalent rotavirus vaccine (Rotarix®) in 2006. This study aimed to assess epidemiology and genotype distribution of species-A (RVA) Brazil, comparing pre- post-vaccination periods.Laboratory-based RVA surveillance included 866 municipalities 22 Brazilian states, over a 21-year period. A total 16,185 children with diarrheal diseases (DD) aged up 12 years between 1996 2005 (pre-vaccination period, n = 7030) from 2006 2017 (post-vaccination 9155) were enrolled. was...

Epidemiological data on species A rotavirus (RVA) infections have demonstrated the genetic diversity of strains circulating worldwide. Many G and P genotype combinations been described over years, varying regionally temporally, especially in developing countries. However, most common identified RVA human worldwide are G1P[8], G2P[4], G3P[8], G4P[8] G9P[8]. G1P[8] responsible for more than 50% child component two vaccines (Rotarix® [RV1] RotaTeq® [RV5]) licensed globally. For a better...

Rotavirus A (RVA) vaccines succeeded in lowering the burden of acute gastroenteritis (AGE) worldwide, especially preventing severe disease and mortality. In 2019, Brazil completed 13 years RVA vaccine implementation (Rotarix™) within National Immunization Program (NIP), as reported elsewhere, use Rotarix™ country has reduced childhood mortality morbidity due to AGE. Even though both marketed are widely distributed, surveillance causing AGE monitoring circulating genotypes important tools...

Human enteric adenoviruses (HAdV-F40/41) play a crucial role as causative agents of acute gastroenteritis (AGE), particularly affecting children in low-and middle-income countries. This study investigated the prevalence, genetic diversity, and molecular characteristics HAdV-F40/41 AGE cases reported Brazil from 2021 to 2023, period after COVID-19 pandemic. A total 1980 stool samples collected medically attended patients nine states were analyzed by TaqMan-based qPCR. Overall, HAdV was...

A total of 614 fecal specimens were obtained during a survey for rotavirus infection conducted between May 1996 and 1998 among 437 newborns admitted to special care nurseries at public hospital in the urban area Belém, Brazil. Routine stool samples taken weekly from all babies up age 28 days. Overall, 51 (11.7%) neonates excreted rotaviruses while hospital, whom 42 (82.3%) developed asymptomatic nosocomial infection; was also proved five nine patients with diarrhea. Three distinct RNA...

Norovirus stands out as a leading cause of acute gastroenteritis (AGE) worldwide, affecting all age groups. In the present study, we investigated fecal samples from medically attended AGE patients received nine Brazilian states, 2019 to 2022, including COVID-19 pandemic period. GI and GII were detected quantified using RT-qPCR, norovirus-positive underwent genotyping through sequencing ORF1/2 junction region. During four-year period, norovirus prevalence was 37.2%, varying 20.1% in 2020...

A case-control study, aimed at identifying factors associated with rotavirus diarrhoea cases presenting to health facilities, was conducted in children from low-income and middle-low-income families Brazil. Cases were 390 stools; controls 1674 without the same facilities. Data collected by questionnaire observations during home visits. Explanatory variables grouped according a conceptual model of causation. The ORs non-conditional logistic regression population-attributable fractions...

This study aims to: estimate the prevalence of G2P[4] rotaviruses in Brazil between 2001-2011 from patients with acute gastroenteritis; perform phylogenetic analyses Brazilian strains (from vaccinated and non-vaccinated children) based on VP7 VP8(∗) encoding genes analyze antigenic regions these proteins comparing RV1; assess full genetic background eleven selected strains. The detection rate among RVA positive samples was 0/157 2001, 3/226 (1.3%) 2002, 0/514 2003, 0/651 2004, 31/344...

Group A rotavirus (RVA) genotype G12 is habitually associated with diarrhea disease (DD) in African children and recently its detection has increased worldwide. total of 970 stool samples collected from individuals DD the Northeastern, Southeastern, Southern Brazilian regions, Eastern coast, were analyzed 321 (33%) positive for RVA these, 241 (75%) genotyped as G12P[8]. The rate positivity was higher among aged 5-10 years old (60%). All infections observed adults >21 G12P[8] (n = 27) showing...

ObjectivesTo verify the frequency of viruses causing acute gastroenteritis (AGE) in association with histo-blood group antigen (HBGA) and Rotarix™ vaccination coverage children from Amazon region.DesignFecal saliva samples were collected AGE (n = 485) respiratory infection (ARI) 249) clinical symptoms. Rotavirus A (RVA), norovirus, human adenovirus (HAdV), sapovirus (SaV) verified feces by molecular detection. Saliva used for HBGA phenotyping/FUT3 genotyping. Blood types, aspects RVA data...

We described the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in stool samples from patients presenting only gastroenteritis (AGE) symptoms. From January to July 2020, 121 AGE were screened by quantitative reverse-transcription polymerase chain reaction. detected SARS-CoV-2 27.5% received during epidemic period. No infectious viruses observed Vero E6 cells.

Rotavirus A (RVA) remains a leading cause of acute gastroenteritis (AGE) hospitalizations in children worldwide. During the COVID-19 pandemic, reduction vaccination coverage Brazil and elsewhere was observed, some reports have demonstrated AGE notifications during pandemic. This study aims to investigate diversity prevalence RVA genotypes adults presenting with symptoms pandemic between 2020 2022. screened using RT-qPCR; then, G P were characterized one-step multiplex RT-PCR. total 2173...

Rotavirus is a major cause of infantile acute diarrhea, causing about 440,000 deaths per year, mainly in developing countries. The World Health Organization has been recommending the assessment rotavirus burden and strain characterization as part strategies immunization programs against this pathogen. In context, prospective study was made on sample 134 children with diarrhea severe dehydration admitted to venous fluid therapy two state hospitals Rio de Janeiro, Brazil, from February...

Abstract Rotavirus epidemiological surveys with molecular analysis of strains are required for gastroenteritis control and prevention. Twenty‐nine human rotavirus detected in Rio de Janeiro, Brazil, from 1986 to 2004 were characterized as P[8],G1, P[8],G5, P[8],G9, P[4],G2 genotypes. The VP7 genes sequenced phylogenetic was performed. Strains genotype G1 revealed two distinct lineages, G1‐3 G1‐4; G2 grouped lineage G2‐1; G5 clustered other Brazilians G9 closely related each G9‐3, the...