A5072989825- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Telomeres, Telomerase, and Senescence
- RNA Interference and Gene Delivery
- Mechanisms of cancer metastasis
- Chromatin Remodeling and Cancer
- Glycosylation and Glycoproteins Research
- Animal Genetics and Reproduction
- Renal and related cancers
- Online Learning and Analytics
- Infant Nutrition and Health
- Career Development and Diversity
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Mentoring and Academic Development
- Cancer-related gene regulation
- Collaborative Teaching and Inclusion
- Histone Deacetylase Inhibitors Research
- Chronic Disease Management Strategies
- Galectins and Cancer Biology
- Amino Acid Enzymes and Metabolism
University of the Sunshine Coast
2022-2025
Sunshine Coast University Hospital
2022-2024
Children's Medical Research Institute
2018-2022
The University of Sydney
2018-2022
Material Sciences (United States)
2020
Kyoto University
2015-2020
Japan Science and Technology Agency
2016
The University of Queensland
2008-2015
International Council for the Exploration of the Sea
2015
Commonwealth Scientific and Industrial Research Organisation
2008-2011
Maternal hyperglycemia is believed to be the metabolic derangement associated with both early pregnancy loss and congenital malformations in a diabetic pregnancy. Using an vitro model of embryo exposure hyperglycemia, this study questioned if increased flux through hexosamine signaling pathway (HSP), which results embryonic O-linked glycosylation (O-GlcNAcylation), underlies glucotoxic effects during embryogenesis. Mouse zygotes were randomly allocated culture treatment groups that included...
Freely accessible generative artificial intelligence (GenAI) poses challenges to physiology education regarding learning and academic integrity. While many studies have explored the capabilities of GenAI complete assessments, few implemented educative activities highlight risks benefits, or students' perceptions uses GenAI. Our study a activity, designed using constructivist principles, allow for students explore consider its use in assessment tasks. The activity engaged (n=236) enrolled...
Abstract Background BRG1 (encoded by SMARCA4 ) is a catalytic component of the SWI/SNF chromatin remodelling complex, with key roles in modulating DNA accessibility. Dysregulation observed, but functionally uncharacterised, wide range malignancies. We have probed functions on background prostate cancer to investigate how controls gene expression programmes and cell behaviour. Results Our investigation revealed that over-expressed majority 486 tumours from The Cancer Genome Atlas cohort, as...
During the transition from pluripotency to a lineage-committed state, chromatin undergoes large-scale changes in structure, involving covalent modification of histone tails, use variants and gene position with respect nuclear periphery. Here, using high-resolution microscopy quantitative image analysis, we surveyed panel modifications for peripheral enrichment during differentiation human embryonic stem cells trophoblast-like lineage. We found two dynamic at periphery, acetylation H2A.Z...
SUMMARY We used super-resolution microscopy to investigate the role of macromolecular telomere structure in chromosome end protection. In murine and human cells with reduced TRF2, we find that ATM-activation at ends occurs a structural change from t-loops linearized through t-loop unfolding. Comparably, Aurora B kinase regulates linearity concurrent ATM activation telomeres during mitotic arrest. Using separation function allele, TRFH domain TRF2 formation while suppressing activity....
Manipulation of mammalian embryos and gametes in vitro reduces viability. Specific causes for these reductions are still largely undetermined. Accumulating evidence suggests that survival rates developmental competency may be reduced following disruptions the epigenetic regulation gene expression. Chromatin-based epigenetics can regulate transcriptome through establishment different transcriptionally permissive repressive chromatin environments. Recently, support has been gathering...
Summary Replication stress is an endemic threat to genome stability. For reasons unknown, replication response factors become essential during peri-implantation development. This coincides with a stem cell potency switch from the naïve primed state. Using genetically matched, chimera-derived mouse embryonic (mESC) and epiblast cells (mEpiSC) we found that management differs between states. Primed mEpiSCs rely on Atr activity prevent catastrophe, minimize genomic damage, avoid apoptosis,...
Histone variants replace canonical histones in nucleosomes to serve numerous biological processes. This integration alters DNA properties ultimately regulate gene expression. Previous mouse studies have indicated that some (H2AZ and H3.3) are essential for survival, but here we document correlate histone expression patterns with key developmental events. Using quantitative reverse-transcribed PCR (qRT–PCR) investigated the of 7 genes coding H2A 4 H3 preimplantation embryos pluripotent R1 ES...
Glucose is an essential signal for metabolic differentiationand blastocyst formation. In its absence compaction disruptedand formation attenuated dramatically. Propagationof this nutrient requires glucose flux through the hexosaminebiosynthetic pathway (HBP) whose main end product aminosugarnucleotide uridine 5'-diphospho-N-acetylglucosamine (UDP-GlcNAc).UDP-GlcNAc acts as a donor substrate multiple glycosylationreactions including Olinked glycosylation, which involves theaddition of single...
During the transition from pluripotency to a lineage-committed state, chromatin undergoes large-scale changes in structure effect required transcriptional program. This involves covalent modification of histone tails, replacement variants, and alteration subnuclear position genes, including associations with nuclear periphery. Here, using high-resolution microscopy quantitative image analysis, we surveyed panel variants modifications for periphery association during differentiation human...
ABSTRACT Background BRG1 (encoded by SMARCA4 ) is a catalytic component of the SWI/SNF chromatin remodelling complex, with key roles in modulating DNA accessibility. Dysregulation observed, but functionally uncharacterised, wide range malignancies. We have probed functions on background prostate cancer to investigate how controls gene expression programs and cell behaviour. Results Our investigation revealed that universally overexpressed 486 tumours from The Cancer Genome Atlas cohort, as...