A5014459683- Plant biochemistry and biosynthesis
- Algal biology and biofuel production
- Biocrusts and Microbial Ecology
- Fungal and yeast genetics research
- Antioxidant Activity and Oxidative Stress
- Photosynthetic Processes and Mechanisms
- Fungal Biology and Applications
- Biofuel production and bioconversion
- Aquatic Ecosystems and Phytoplankton Dynamics
- Microbial Metabolism and Applications
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Fermentation and Sensory Analysis
- Enzyme Production and Characterization
University of Chile
2019-2024
Xanthophyllomyces dendrorhous is a basidiomycete yeast that synthesizes carotenoids, mainly astaxanthin, which are of great commercial interest. Currently, there many unknown aspects related to regulatory mechanisms on the synthesis carotenoids in this yeast. Our recent studies showed changes sterol levels and composition resulted upregulation genes mevalonate pathway required for carotenoid precursors, leading increased production these pigments. Sterol Regulatory Element-Binding Proteins...
The Phaffia rhodozyma UCD 67-385 genome harbors a 7873 bp cluster containing DDGS, OMT, and ATPG, encoding 2-desmethy-4-deoxygadusol synthase, O-methyl transferase, ATP-grasp ligase, respectively, of the mycosporine glutaminol (MG) biosynthesis pathway. Homozygous deletion mutants entire cluster, single-gene mutants, Δddgs-/-;Δomt-/- Δomt-/-;Δatpg-/- double-gene did not produce mycosporines. However, Δatpg-/- accumulated intermediate 4-deoxygadusol. Heterologous expression DDGS OMT or ATPG...
Xanthophyllomyces dendrorhous is a basidiomycete yeast that produces carotenoids, mainly astaxanthin. Astaxanthin an organic pigment of commercial interest due to its antioxidant and coloring properties. X. has functional SREBP pathway, the Sre1 protein homolog in this yeast. However, how sterol regulatory element (Sre)1 promotes biosynthesis sterols carotenoids unknown. In work, comparative RNA-sequencing analysis between modified strains have active WT was performed identify Sre1-dependent...
Xanthophyllomyces dendrorhous is a natural source of astaxanthin and mycosporines. This yeast has been isolated from high cold mountainous regions around the world, production these secondary metabolites may be survival strategy against stress conditions present in its environment. Biosynthesis regulated by catabolic repression through interaction between MIG1 corepressor CYC8-TUP1. To evaluate role stress-associated transcription factors SKN7, ROX1, YAP6, we employed an omic phenotypic...
Xanthophyllomyces dendrorhous is a carotenogenic yeast with singular metabolic capacity to produce astaxanthin, valuable antioxidant pigment. This can assimilate several carbon sources and sustain fermentation even under aerobic conditions. Since astaxanthin biosynthesis affected by the source, study of carotenogenesis regulatory mechanisms key for improving yield in X. aimed elucidate regulation metabolism different phenomenon catabolic repression this yeast. To end, protein transcript...
The sterol regulatory element-binding protein (SREBP) pathway is an integral cellular mechanism that regulates lipid homeostasis, in which transcriptional activator SREBPs regulate the expression of various genes. In carotenogenic yeast