A5081346191- Erythrocyte Function and Pathophysiology
- Platelet Disorders and Treatments
- RNA modifications and cancer
- Bone health and treatments
- Acute Myeloid Leukemia Research
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Hematopoietic Stem Cell Transplantation
- Blood properties and coagulation
- Chronic Lymphocytic Leukemia Research
- Cancer-related gene regulation
- Bone Metabolism and Diseases
- Blood groups and transfusion
- Chronic Myeloid Leukemia Treatments
- Iron Metabolism and Disorders
- Nuclear Structure and Function
- Endoplasmic Reticulum Stress and Disease
- Cell Image Analysis Techniques
- Microfluidic and Bio-sensing Technologies
- Cancer, Stress, Anesthesia, and Immune Response
- Eicosanoids and Hypertension Pharmacology
- Neuroblastoma Research and Treatments
- Advanced Biosensing Techniques and Applications
- Heterotopic Ossification and Related Conditions
- Cancer Genomics and Diagnostics
UConn Health
2014-2024
Universidad Cristiana Autónoma de Nicaragua
2024
Yale Cancer Center
2019-2023
Yale University
2017-2023
University of Connecticut
2023
Abstract Single-cell assays have enriched our understanding of hematopoiesis and, more generally, stem and progenitor cell biology. However, these single-end-point approaches provide only a static snapshot the state cell. To observe measure dynamic changes that may instruct fate, we developed an approach for examining hematopoietic fate specification using long-term (> 7-day) single-cell time-lapse imaging up to 13 generations with in situ fluorescence staining primary human progenitors...
Cadherins play a major role in mediating cell-cell adhesion, which shares many parallels with platelet-platelet interactions during aggregate formation and clot stabilization. Platelets express epithelial (E)-cadherin, but its contribution to platelet function and/or production is currently unknown. To assess the of E-cadherin vitro vivo, we utilized megakaryocyte-specific knockout mouse model. Loss megakaryocytes does not affect megakaryocyte maturation, number or size. However, dysfunction...
Diamond-Blackfan Anemia (DBA) is an inherited bone marrow failure syndrome that associated with anemia, congenital anomalies, and cancer predisposition. It categorized as a ribosomopathy, because more than 80% or patients have haploinsufficiency of either small large subunit-associated ribosomal protein (RP). The erythroid pathology due predominantly to block delay in early committed erythropoiesis reduced megakaryocyte/erythroid progenitors (MEPs). To understand the molecular pathways...
Mice in which Cbl is unable to bind PI3K (YF mice) display increased bone volume due enhanced formation and repressed resorption during normal homeostasis. We investigated the effects of disrupted Cbl-PI3K interaction on fracture healing determine whether this has an effect repair. Mid-diaphyseal femoral fractures induced wild type (WT) YF mice were temporally evaluated via micro-computed tomography scans, biomechanical testing, histological histomorphometric analyses. Imaging analyses...
Hematopoietic stem and progenitor cell (HSPC) transplantation is an essential therapy for hematological conditions, but finer definitions of human HSPC subsets with associated function could enable better tuning grafts more routine, lower-risk application. To deeply phenotype HSPCs, following a screen 328 antigens, we quantified 41 surface proteins functional regulators on millions CD34+ CD34- cells, spanning four primary hematopoietic tissues: bone marrow, mobilized peripheral blood, cord...
Diamond Blackfan anemia (DBA) is an inherited bone marrow failure syndrome associated with severe anemia, congenital malformations, and increased risk of developing cancer. The chromatin-binding special AT-rich sequence-binding protein-1 (SATB1) downregulated in megakaryocyte/erythroid progenitors (MEPs) patients cell models DBA, leading to a reduction MEP expansion. Here we demonstrate that SATB1 expression required for the upregulation critical erythroid factors heat shock protein 70...
Abstract The intricate interplay between the nervous and immune systems significantly influences fate of cells in homeostasis as well under pathological conditions such cancer. Notably, adrenergic signals from sympathetic system have been identified regulators hematopoiesis. Disruption signaling has linked to mal-differentiation myeloid lymphoid lineages leading emergence Tregs, myeloid-derived suppressor cells, exhaustion CD8+ T cells. We show here that disrupted also leads erythroid...
Searchable abstracts of presentations at key conferences on calcified tissues ISSN 2052-1219 (online)
Abstract Single-cell assays have enriched our understanding of hematopoiesis and, more generally, stem and progenitor cell biology. However, these single-end-point approaches provide only a static snapshot the state cell. To observe measure dynamic changes that may instruct fate, we developed an approach for examining hematopoietic fate specification using long-term (> 7-day) single-cell time-lapse imaging up to 13 generations with in situ fluorescence staining primary human progenitors...
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