A5040717069- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Cellular transport and secretion
- Hip and Femur Fractures
- Trauma and Emergency Care Studies
- Pelvic and Acetabular Injuries
- Advanced biosensing and bioanalysis techniques
- Galectins and Cancer Biology
- Biochemical Analysis and Sensing Techniques
- Glycosylation and Glycoproteins Research
- Genetic factors in colorectal cancer
- Biochemical effects in animals
- Lymphoma Diagnosis and Treatment
- Colorectal and Anal Carcinomas
- Signaling Pathways in Disease
- Meat and Animal Product Quality
University of Cambridge
2002-2021
Cambridge University Hospitals NHS Foundation Trust
2021
Addenbrooke's Hospital
2021
Galectins are a family of lectin binding proteins expressed both intracellularly and extracellularly. Galectin-3 (Gal-3, also known as LGALS3) is at the cell surface; however, Gal-3 lacks signal sequence, mechanism transport to surface remains poorly understood. Here, using genome-wide CRISPR/Cas9 forward genetic screen for regulators localization, we identified genes encoding glycoproteins, enzymes involved in N-linked glycosylation, ER-Golgi trafficking immunity. The results this screening...
Background: The fundamental process of protein secretion from eukaryotic cells has been well described for many years, yet gaps in our understanding how this is regulated remain. Methods: With the aim identifying novel genes involved glycoproteins, we used a screening pipeline consisting pooled genome-wide CRISPR screen, followed by secondary siRNA hits to identify and validate several regulators secretion. Results: We present approximately 50 not previously associated with secretion, which...
This study aimed to investigate potential factors, including delay surgical stabilisation, affecting mortality in older patients sustaining pelvic or acetabular (PA) fractures.A retrospective review of the Trauma Audit and Research Network (TARN) database was performed identify (aged 65 over) PA fractures treated surgically a UK Major Centre (MTC) between 2015 2019. Chi-squared Fisher tests were used compare 1-year rates following operative intervention within 72 h after h. Kaplan-Meier...
<ns5:p><ns5:bold>Background:</ns5:bold> The fundamental process of protein secretion from eukaryotic cells has been well described for many years, yet gaps in our understanding how this is regulated remain.</ns5:p><ns5:p> <ns5:bold>Methods:</ns5:bold> With the aim identifying novel genes involved glycoproteins, we used a screening pipeline consisting pooled genome-wide CRISPR screen, followed by secondary siRNA hits to identify and validate several regulators secretion.</ns5:p><ns5:p>...
ABSTRACT The fundamental process of protein secretion from eukaryotic cells has been well described for many years, yet gaps in our understanding how this is regulated remain. With the aim identifying novel genes involved glycoproteins, we used a screening pipeline consisting pooled genome-wide CRISPR screen followed by secondary siRNA hits to identify and validate several regulators secretion. We present approximately 50 not previously associated with secretion, which also had an effect on...
Abstract Introduction This study aimed to investigate potential factors, including delay surgical stabilisation, affecting mortality in older patients sustaining pelvic or acetabular (PA) fractures. Method A retrospective review of the Trauma Audit and Research Network (TARN) database was performed identify (aged 65 over) PA fractures treated surgically a UK Major Centre (MTC) between 2015 2019. Chi-squared Fisher tests were used compare 1-year rates following operative intervention within...