A5020027137- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetics and Neurodevelopmental Disorders
- RNA regulation and disease
- Epigenetics and DNA Methylation
- Bacteriophages and microbial interactions
- HVDC Systems and Fault Protection
- Chromosomal and Genetic Variations
- Genetics, Bioinformatics, and Biomedical Research
- Biomedical and Engineering Education
- Photoreceptor and optogenetics research
- Peptidase Inhibition and Analysis
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Protein Degradation and Inhibitors
- interferon and immune responses
- Cardiac Structural Anomalies and Repair
- Sexual Differentiation and Disorders
University of Oxford
2017-2025
Hangzhou Normal University
2022-2024
Chinese Academy of Sciences
2011-2020
Institute of Biophysics
2011-2020
University of Chinese Academy of Sciences
2012-2015
Institute of Computing Technology
2011
Chinese National Human Genome Center
2011
Institute of Zoology
2011
Max Planck Institute of Immunobiology and Epigenetics
1997
Abstract Xist RNA, the master regulator of X chromosome inactivation, acts in cis to induce chromosome-wide silencing. Whilst recent studies have defined candidate silencing factors, their relative contribution repressing different genes, and relationship with one another is poorly understood. Here we describe a systematic analysis Xist-mediated allelic mouse embryonic stem cell-based models. Using machine learning approach identify distance locus prior gene expression levels as key...
Tripartite motif (TRIM) family proteins participate in a variety of important cellular processes, including apoptosis, cell-cycle arrest, DNA repair, and senescence. In this study, we demonstrated that novel TRIM member, TRIM67, was commonly silenced colorectal cancer its downregulation associated with poor survival. Trim67 knockout ApcMin/+ mice increased the incidence, multiplicity, burden tumors. Similarly, colon-specific significantly accelerated azoxymethane-induced mice. RNA sequencing...
Visualizing Xist RNA dynamics The noncoding Xist, which controls the process of X chromosome inactivation in mammals, accumulates and spreads over from it is transcribed. underlying basis for this unusual behavior poorly understood. Using a new imaging approach called RNA-SPLIT time-resolved analysis molecules at super-resolution, Rodermund et al. analyzed fundamental parameters normal cells after perturbation factors implicated function. authors provide insights into localization...
RNA N6-methyladenosine (m6A) modification plays important roles in multiple aspects of regulation. m6A is installed cotranscriptionally by the METTL3/14 complex, but its direct processing remain unclear. Here, we investigate presence nascent mouse embryonic stem cells. We find that around 10% peaks are located alternative introns/exons, often close to 5' splice sites. significantly overlap with RBM15 binding sites and histone H3K36me3. Acute depletion METTL3 disrupts inclusion introns/exons...
Accumulating evidence suggests that long noncoding RNAs (lncRNAs) play important roles in transcriptional regulation, whereas the extent to which lncRNAs also function at posttranscriptional level is less known. In present study, we report a lncRNA named MT1JP acts as tumor suppressor through mechanism. We found differentially expressed tissues by analyzing data from customized microarray applied 76 pairs of matched normal and cancer tissue samples. By associating with RNA-binding protein...
<ns4:p><ns4:bold>Background</ns4:bold>: X chromosome inactivation in mammals is regulated by the non-coding (nc) RNA, Xist, which represses from it transcribed. High levels of N6-methyladenosine (m6A) RNA modification occur within Xist exon I, close to 5’ end transcript, and also further 3’, VII. The m6A catalysed METTL3/14 complex that directed specific targets, including binding protein RBM15/15B. has been reported be important for Xist–mediated gene silencing. </ns4:p><ns4:p>...
Transcriptional regulation by chromatin is a highly dynamic process directed through the recruitment and coordinated action of epigenetic modifiers readers these modifications. Using an unbiased proteomic approach to find interactors H3K36me3, modification enriched on active chromatin, here we identify PWWP2A HDAC2 among top interactors. its paralog PWWP2B form stable complex with NuRD subunits MTA1/2/3:HDAC1/2:RBBP4/7, but not MBD2/3, p66α/β, CHD3/4. competes MBD3 for binding MTA1, thus...
X chromosome inactivation (XCI) is mediated by the non-coding RNA Xist, which directs chromatin modification and gene silencing in cis. The binding protein SPEN associated corepressors have a central role Xist-mediated silencing. Other factors, notably Polycomb system, been reported to function downstream of SPEN. In recent work, we found that has an additional correct localization Xist cis, indicating its contribution chromatin-mediated needs be reappraised. Making use...
Abstract Pseudouridine (Ψ) is one of the most abundant modifications in cellular RNA. However, its function remains elusive, mainly due to lack highly sensitive and accurate detection methods. To address this challenge, we introduced 2-bromoacrylamide-assisted cyclization sequencing (BACS) for quantitative profiling Ψ at single-base resolution. Based on novel bromoacrylamide chemistry, BACS enables a Ψ-to-C transition. Compared previous methods, allowed precise identification positions,...
Rearranged during transfection (RET) rearrangement oncoprotein-mediated Ras/MAPK signaling cascade is constitutively activated in cancers. Here, we demonstrate a unique signal niche. The niche ternary complex based on the chimeric RET liquid–liquid phase separation. comprises rearranged kinase (RET fusion); adaptor (GRB2), and effector (SHC1). Together, they orchestrate cascade, which dependent tyrosine kinase. CCDC6-RET fusion undergoes LLPS requiring its domain partner. promotes...
Abstract X chromosome inactivation (XCI) in mammals is orchestrated by the non-coding RNA Xist which together with specific interacting proteins, functions cis to silence an entire chromosome. Defined sites on carry N 6 -methyladenosine (m A) modification, and perturbation of m A writer complex has been found abrogate Xist-mediated gene-silencing. However, relative contribution its mechanism action remain unclear. Here we investigate role XCI applying rapid degron-mediated depletion METTL3,...
// Guifeng Wei 1,2,* , Huaxia Luo Yu Sun Jiagen Li 3 Liqing Tian 1 Liu 1,2 Lihui Jianjun Jie He and Runsheng Chen 1,4 Bioinformatics Laboratory CAS Key of RNA Biology, Institute Biophysics, Chinese Academy Sciences, Beijing, China 2 University Department Thoracic Surgery, Cancer Hospital, Medical Sciences Peking Union College, 4 Research Network Computational RNCB, * These authors have contributed equally to this work Correspondence to: Chen, email: He, Keywords : ESCC, long noncoding RNAs,...
The wide variety of specialized permissive and repressive mechanisms by which germ cells regulate developmental gene expression are not well understood genome-wide. Isolation with high integrity purity from living animals is necessary to address these open questions, but no straightforward methods currently available. Here we present an experimental paradigm that permits the isolation nuclei C. elegans at quantities sufficient for genomic analyses. We demonstrate represent a very pure...
Long noncoding RNAs (lncRNAs) are pervasively transcribed in the human genome. Recent studies suggest that involvement of lncRNAs diseases could be far more prevalent than previously appreciated. Here we have identified a lncRNA termed Lnc_bc060912 whose expression is increased lung and other tumors. 1.2 kb length composed two exons. The was repressed by p53. suppressed cell apoptosis. Using recently developed method for RNA-pulldown with formaldehyde cross-linking, found interacted DNA...
In the last decade, it has been demonstrated that brain functional asymmetry occurs not only in vertebrates but also invertebrates. However, mechanisms underlying remain unclear. present study, we trained honeybees of same parentage and age, on proboscis extension reflex (PER) paradigm with one antenna use. The comparisons gene expression between left right hemispheres were carried out using high throughput sequencing. Our research revealed honeybee is asymmetric, more genes having higher...
Abstract The identification and characterization of long non-coding RNAs (lncRNAs) in diverse biological processes has recently developed rapidly. large amounts scale consistent with developmental complexity eukaryotes, indicating that most these transcripts may have functions the regulation disorder organisms. In particular, Understanding overall significance lncRNAs cancers still remains limited. Here, we found a nuclear-retained lncRNA, termed Lnc_ASNR (apoptosis suppressing-noncoding...