A5102766253- Optical Coherence Tomography Applications
- Tree Root and Stability Studies
- Plant Molecular Biology Research
- Photoacoustic and Ultrasonic Imaging
- Orthopedic Infections and Treatments
- Metal and Thin Film Mechanics
- Polysaccharides and Plant Cell Walls
- Photodynamic Therapy Research Studies
- Advanced ceramic materials synthesis
- Advanced materials and composites
- Bacterial biofilms and quorum sensing
- Polysaccharides Composition and Applications
- Biocrusts and Microbial Ecology
- Nanoplatforms for cancer theranostics
- Cancer Research and Treatments
- Advanced Fluorescence Microscopy Techniques
- Wound Healing and Treatments
- Antimicrobial Peptides and Activities
- Antimicrobial Resistance in Staphylococcus
- Ocular Infections and Treatments
- Cell Image Analysis Techniques
- Angiogenesis and VEGF in Cancer
- Pharmacological Effects of Natural Compounds
- Ginger and Zingiberaceae research
- Spectroscopy Techniques in Biomedical and Chemical Research
Dartmouth–Hitchcock Medical Center
2025
Dartmouth Psychiatric Research Center
2025
Dartmouth Health
2025
Laboratoire d'Hydrodynamique
2022-2024
École Polytechnique
2023-2024
Centre National de la Recherche Scientifique
2023-2024
University of Toronto
2021-2024
Massachusetts Institute of Technology
2023
Massachusetts General Hospital
2023
Harvard University
2023
SignificanceIn orthopedic trauma surgery, spatially structured biofilm ecosystems of bacteria that colonize devices account for up to 65% all healthcare infections, including tens millions people affected in the United States. These infections typically show increased resistance antibiotics due their structure and composition, which contributes significantly treatment failure. Anti-biofilm approaches are needed together with clinically usable microscopic-resolution imaging techniques...
Orthopedic implant-associated infections cause serious complications primarily attributed to bacterial biofilm formation and are often characterized by increased antibiotic resistance diminished treatment response. Yet, no methods currently exist identify biofilms intraoperatively-surgeons rely solely on their eyes hands cannot detect or differentiate infected tissue determine the location extent of contamination. As first step in addressing this unmet clinical need, here, we develop an...
Texture analyses of optical coherence tomography (OCT) images have shown initial promise for differentiation normal and tumor tissues. This work develops a fully automatic volumetric delineation technique employing quantitative OCT image speckle analysis based on Gamma distribution fits. We test its performance in-vivo using immunodeficient mice with dorsal skin window chambers subcutaneously grown models. Tumor boundaries detection is confirmed epi-fluorescence microscopy, combined...
Multicellular organisms grow and acquire their shapes through the differential expansion deformation of cells. Recent research has addressed role cell tissue mechanical properties in these processes. In plants, it is believed that growth rate a function stress exerted on wall, thin polymeric layer surrounding cells, involving an effective viscosity. Nevertheless, recent studies have questioned this view, suggesting wall elasticity sets or uptake water limiting for plant growth. To assess...
Orthopaedic implant-associated infections cause serious complications primarily attributed to bacterial biofilm formation and often characterized by increased antibiotic resistance diminished treatment response. There is currently a lack of imaging modalities that can directly visualize biofilms determine the location extent contamination. Optical coherence tomography (OCT) portable, non-invasive, high-resolution modality with potential fulfill this unmet need. In study, we aim evaluate...
The issue of radiation damage to skin in conventional radiotherapy (RT) has motivated pre-clinical studies demonstrate reduced toxicity and overall improved normal tissue sparing high-dose rate FLASH RT. We address the critical need for diagnostic tools evaluate radiobiological response mechanisms produced with RT by using low-coherence light scattering quantify tumor versus radiotherapy. A study melanoma growth dorsal window chambers on rodents vivo following separate regiments treatment...
Methicillin-resistant <i>S. aureus</i> (MRSA) bacteria commonly found on orthopaedic implants, form treatment resistant biofilms that are difficult to manage. Creating new imaging modalities allow us understand biofilm development and accurately indicate the efficacy of treatments will greatly aid research in infection methods. In this vitro study, we determined correlation between number MRSA CFUs radiance aliquots with bioluminescent plasmids resolution volume Perkin Elmer's IVIS Spectrum...
Here we report the methodology development and first clinical translation of optical coherence tomography-based detection Methicillin-resistant Staphylococcus aureus (MRSA) biofilms developed on bone surface after open tibia fracture surgery.
Abstract Multicellular organisms grow and acquire their shapes through the differential expansion deformation of cells. Recent research has addressed role cell tissue mechanical properties in these processes. In plants, it is believed that growth rate a function stress exerted on wall, thin polymeric layer surrounding cells, involving an effective viscosity. Nevertheless, recent studies have questioned this view, suggesting wall elasticity sets or uptake water limiting for plant growth. To...
Well-organized ecosystems of bacteria colonize orthopaedic devices causing biofilm infections that are notoriously difficult to manage. Biofilms typically exhibit increased resistance antibiotics leading treatment failure, and tools for eradicating biofilms do not increase antibiotic greatly needed. Antimicrobial photodynamic therapy (aPDT) is a promising form combat clinically relevant biofilms. Exogenous provision 5-aminolevulinic acid (5-ALA) biofilm-forming clinical strains <i>E....
Adnexal structures in skin, such as sweat glands and hair follicles, harbor stem cells that are indispensable for wound healing following acute injuries burns of various degrees [1]. Developing a method to identify visualize these the dermal layer tissue would greatly supplement imaging studies probing burn injury depth-extent, assess whether intact adnexal will contribute provide invaluable prognostic information clinic. Two methods were recently attempted this purpose literature but held...