A5101433117- Microbial Metabolic Engineering and Bioproduction
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Pharmacological Effects of Natural Compounds
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Gene Regulatory Network Analysis
- Bacterial Identification and Susceptibility Testing
- Enzyme Catalysis and Immobilization
- Microbial Natural Products and Biosynthesis
- Biofuel production and bioconversion
- Biochemical and Molecular Research
- Genetics and Neurodevelopmental Disorders
- Chromatography in Natural Products
- Synthesis and biological activity
- Nanoplatforms for cancer theranostics
- Axon Guidance and Neuronal Signaling
- Chemical Synthesis and Analysis
- Plant Pathogenic Bacteria Studies
- RNA Interference and Gene Delivery
- Pharmaceutical and Antibiotic Environmental Impacts
- Enterobacteriaceae and Cronobacter Research
- Phytochemistry and biological activity of medicinal plants
University of Georgia
2020-2025
Lawrence Berkeley National Laboratory
2025
Wenzhou Medical University
2012-2024
The First People's Hospital of Wenling
2018-2024
Affiliated Eye Hospital of Wenzhou Medical College
2016-2024
Sir Run Run Shaw Hospital
2022-2023
Zhejiang University
2022-2023
Ministry of Industry and Information Technology
2021
Beijing Institute of Technology
2018-2021
Chinese Academy of Sciences
2020
Abstract The abundance of bacterial effectors have inspired us to explore their potential in rewiring malignant cell signaling. Their incapability for entering cells, however, hinders such application. Herein we developed a cationic lipid‐based high throughput library screening platform effective intracellular delivery effectors. As the misregulated MAPK signaling is hallmark many types cancer, turned Shigella effector OspF which irreversibly inactivates ERK, terminal component cascade. We...
To achieve low-carbon bioeconomy, we established a carbon-efficient bioproduction approach by fine-tuning non-glycolysis pathway, which reduces CO 2 emission and balances the acetyl-CoA redox supply from sugar breakdown to pathway demand.
By combining protein engineering and synthetic biology strategies, the novel de novo biosynthesis of 4,6-dihydroxycoumarin from glycerol was realized in Escherichia coli for first time.
ABSTRACT Escherichia coli accumulates acetate as a byproduct in fast growth aerobic conditions when using glucose carbon source. This phenomenon, known overflow metabolism, has negative impacts on cell and protein expression, also causes loss during biosynthesis most microbial production scenarios. In this study, we regarded the “waste” metabolite useful metabolism indicator, constructed an biosensor to monitor change of concentration converted signal into various regulation outputs....
Compared to chemical methods, the biotransformation of glycyrrhizin (GL) into glycyrrhetinic acid 3- O -mono- β - d -glucuronide (GAMG), which has a higher sweetness and stronger pharmacological activity than those GL, via catalysis by -glucuronidase is an environmentally friendly approach due mild reaction conditions high yield GAMG. However, currently available GUSs show low substrate specificity toward GL further hydrolyze GAMG (GA) as by-product, increasing difficulty subsequent...
Metabolic engineering is a promising approach for the synthesis of valuable compounds. Transcriptional factor-based biosensors are efficient tools to regulate metabolic pathway dynamically. Here, we engineered p-coumaric acid responsive regulator PadR from Bacillus subtilis. We found that yveF and yveG, two previously uncharacterized components in sensor system, showed positive impacts on regulation PadR-PpadC mostly assisting release repression by PadR. By site directed engineering,...
To improve the isoprenol pathway performance and yield, we exploited CRISPRi screening to identify endogenous gene targets involving phosphate intermediate hydrolysis acetyl–CoA consumption pathways toxicity in E. coli .
Prevalence of carbapenem-resistant Klebsiella pneumoniae (CRKP) has compromised antimicrobial efficacy against severe infections worldwide. To monitor global spread, we conducted a comprehensive genomic epidemiologic study comparing sequences from 21 blaOXA-232-carrying CRKP isolates China with K. sequence type (ST) 15 strains 68 countries available in GenBank. Phylogenetic and phylogeographic analyses revealed all belonged to ST15 lineage exhibited multidrug resistance. Analysis grouped 330...
Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen contributing to healthcare-associated infections, which can result in multiple sites infections. The epidemiological characteristics of MRSA exhibit variability among distinct regions and healthcare facilities. aim this study was investigate the molecular epidemiology nosocomial outbreak county-level hospital China. A total 130 non-repetitive strains were collected from December 2020 November 2021. Whole-genome...
Abstract Autism spectrum disorder (ASD) is a complex neurodevelopmental that has strong genetic component. Disruptions of FOXP1, transcription factor expressed in the developing cerebral cortex, were associated with ASD. FOXP1(R525X) de novo heterozygous mutation found patients autism and severe mental retardation. To explore neuronal basis ASD, we created Foxp1(R521X), mouse homolog human variant. Ectopic expression Foxp1(R521X) led to cytoplasmic aggregates activated macroautophagy...
Klebsiella michiganensis is an emerging hospital-acquired bacterial pathogen. However, there a dearth of knowledge on the antimicrobial resistance and transmission K. michiganensis. Here, we characterized microbiological genomic features carbapenem-resistant strain harboring blaNDM-1 gene in China.K. 2563 was recovered from sputum sample hospitalized patient with pulmonary infection. Whole-genome sequencing conducted using both short-read Illumina long-read MinION platforms to thoroughly...
Colistin is considered as one of the last-resort antimicrobial agents for treating multidrug-resistant bacterial infections. Multidrug-resistant E. asburiae has been increasingly isolated from clinical patients, which posed a great challenge antibacterial treatment. This study aimed to report mcr-10 and blaNDM-1 co-carrying isolate 5549 conferred high-level resistance against colistin. Antibiotic susceptibility testing was performed using microdilution broth method. Transferability...