A5021681939- Phosphodiesterase function and regulation
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Adipose Tissue and Metabolism
- Advanced Fluorescence Microscopy Techniques
- Advanced Biosensing Techniques and Applications
- Synthesis and Biological Evaluation
- Photoreceptor and optogenetics research
- Nitric Oxide and Endothelin Effects
- ATP Synthase and ATPases Research
- Spaceflight effects on biology
- Chemical Synthesis and Analysis
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Biological Research and Disease Studies
- Neuroscience and Neuropharmacology Research
- Agriculture and Agroindustry Studies
- Agricultural and Environmental Management
- Cell Image Analysis Techniques
- Click Chemistry and Applications
- Management and Optimization Techniques
Waseda Bioscience Research Institute in Singapore
2016-2021
cAMP is a common second messenger that involved in various physiological processes. To expand the colour palette of available indicators, we developed red indicator named "Pink Flamindo" (Pink Fluorescent indicator). The fluorescence intensity Pink Flamindo increases 4.2-fold presence saturating dose cAMP, with excitation and emission peaks at 567 nm 590 nm, respectively. Live-cell imaging revealed effective for monitoring spatio-temporal dynamics intracellular generated by photoactivated...
Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing spatiotemporal dynamics ATP single cells is fundamental to our understanding mechanisms underlying metabolism. However, it has remained challenging visualize and between distinct intracellular organelles its interplay with other signaling molecules. Using fluorescent proteins, multicolor indicators were developed, enabling simultaneous visualization subcellular...
Fluorescent probes are valuable tools for visualizing the spatiotemporal dynamics of molecules in living cells. Here we developed a genetically encoded antibody probe with antigen-dependent fluorescence intensity called "Flashbody". We first created fusion EGFP to single chain variable region fragment (scFv) against seven amino acids bone Gla protein C-terminus (BGPC7) BGP Fluobody, which successfully showed intracellular localization BGPC7-tagged protein. To generate Flashbody, circularly...
Here we describe the development of a single fluorescent protein (FP)-based cGMP indicator, Green cGull, based on binding domain from mouse phosphodiesterase 5α. The dynamic range cGull was enhanced to 7.5-fold fluorescence change upon by optimization amino acid linkers between and FP. has excitation emission peaks at 498 522 nm, respectively, specifically responds in dose-dependent manner. Live cell imaging analysis revealed that addition nitric oxide (NO) donor induced different kinetics...
Abstract Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing spatiotemporal dynamics ATP single cells is fundamental to our understanding mechanisms underlying metabolism. However, it has remained challenging visualize and between distinct intracellular organelles its interplay with other signaling molecules. Using fluorescent proteins, multicolor indicators were developed, enabling simultaneous visualization...