A5009609283- Plant Genetic and Mutation Studies
- Genomics and Chromatin Dynamics
- Plant Molecular Biology Research
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Agriculture, Soil, Plant Science
- Plant responses to water stress
- Genomics and Rare Diseases
- Plant Gene Expression Analysis
- Cancer-related gene regulation
- Light effects on plants
- Plant nutrient uptake and metabolism
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
John Innes Centre
2021-2024
Norwich Research Park
2021-2024
The mechanisms and timescales controlling de novo establishment of chromatin-mediated transcriptional silencing by Polycomb repressive complex 2 (PRC2) are unclear. Here, we investigate PRC2 at Arabidopsis FLOWERING LOCUS C (FLC), known to involve co-transcriptional RNA processing, histone demethylation activity, function, but so far not mechanistically connected. We develop test a computational model describing proximal polyadenylation/termination mediated the RNA-binding protein FCA that...
The histone modification H3K27me3 plays a central role in Polycomb-mediated epigenetic silencing. recruits and allosterically activates Polycomb Repressive Complex 2 (PRC2), which adds this to nearby histones, providing read/write mechanism for inheritance through DNA replication. However, some PRC2 targets, purely histone-based system may be insufficient. We address issue at the target FLOWERING LOCUS C (FLC) Arabidopsis thaliana , as narrow nucleation region of only ~three nucleosomes...
Summary Throughout their lifecycle, plants are subjected to DNA damage from various sources, both environmental and endogenous. Investigating the mechanisms of response (DDR) is essential unravel how adapt changing environment, which can induce varying amounts damage. Using a combination whole‐mount single‐molecule RNA fluorescence in situ hybridization (WM‐smFISH) plant cell cycle reporter lines, we investigated transcriptional activation key homologous recombination (HR) gene, RAD51 ,...
Report24 October 2022Open Access Transparent process The plant-specific DDR factor SOG1 increases chromatin mobility in response to DNA damage Anis Meschichi orcid.org/0000-0001-8946-6023 Plant Biology Department, Swedish University of Agricultural Sciences, Uppsala, Sweden Contribution: Conceptualization, Formal analysis, Investigation, Writing - original draft, review & editing Search for more papers by this author Lihua Zhao orcid.org/0000-0002-1758-9873 Investigation Svenja Reeck John...
Quantitative gene regulation at the cell population level can be achieved by two fundamentally different modes of individual copies. A ‘digital’ mode involves binary ON/OFF expression states, with population-level variation arising from proportion copies in each state, while an ‘analog’ graded levels copy. At Arabidopsis floral repressor FLOWERING LOCUS C (FLC), Polycomb silencing is known to facilitate quantitative epigenetic memory response cold. However, whether FLC before cold analog or...
Chromatin-mediated transcriptional silencing by Polycomb Repressive Complex 2 (PRC2) is critical for gene regulation in development and environmental responses. However, the mechanism timescales controlling de novo establishment of PRC2 are unclear. Here, we investigate at Arabidopsis FLOWERING LOCUS C (FLC), known to involve co-transcriptional RNA processing, histone demethylation activity, function; but so far not mechanistically connected. We develop then test a computational model that...
SUMMARY Homologous recombination (HR) is a conservative DNA repair pathway in which intact homologous sequences are used as template for repair. How the homology search happens crowded space of cell nucleus is, however, still poorly understood. Here, we measured global chromosome and double-strand break (DSB) site mobility Arabidopsis thaliana , using lacO /LacI lines two GFP-tagged HR reporters. We observed an increase chromatin upon induction damage, specifically at S/G2 phases cycle. DSB...
Throughout their lifecycle, plants are subjected to DNA damage from various sources, both environmental and endogenous. Investigating the mechanisms of response (DDR) is essential unravel how adjust changing environment that can elicit varying amounts damage. Using a combination state-of-the-art cell biology methods including whole-mount single- molecule RNA fluorescence in situ hybridization (WM-smFISH), allowing detection individual mRNA molecules intact plant tissue cycle reporter lines...
Abstract Quantitative gene regulation at the cell population-level can be achieved by two fundamentally different modes of individual copies. A “digital” mode involves binary ON/OFF expression states, with variation arising from proportion copies in each state, while an “analog” graded levels copy. At Arabidopsis floral repressor FLOWERING LOCUS C (FLC), Polycomb silencing is known to facilitate quantitative epigenetic memory response cold. However, whether FLC before cold analog or digital...
Chromatin-mediated transcriptional states are central to gene regulation in development and environmental response, with co-transcriptional processes involved their establishment. Quantitative of Arabidopsis FLOWERING LOCUS C (FLC) is key determining reproductive strategy. Low FLC expression underpins rapid-cycling established through a transcription-coupled chromatin mechanism. Proximal termination antisense transcripts linked histone 3 lysine 4 demethylation adjacent that leads stable...