Alok Das

ORCID: 0000-0002-6669-9147
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About
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Research Areas
  • Genetic and Environmental Crop Studies
  • Agricultural pest management studies
  • Legume Nitrogen Fixing Symbiosis
  • Plant Genetic and Mutation Studies
  • Plant-Microbe Interactions and Immunity
  • Plant tissue culture and regeneration
  • Plant Disease Resistance and Genetics
  • Insect Resistance and Genetics
  • Plant Pathogens and Fungal Diseases
  • Fungal and yeast genetics research
  • Genetically Modified Organisms Research
  • GABA and Rice Research
  • Seed Germination and Physiology
  • Plant pathogens and resistance mechanisms
  • Biodiesel Production and Applications
  • Banana Cultivation and Research
  • Cassava research and cyanide
  • Transgenic Plants and Applications
  • Algal biology and biofuel production
  • Plant nutrient uptake and metabolism
  • Vibrio bacteria research studies
  • Plant Pathogenic Bacteria Studies
  • Phytase and its Applications
  • Nematode management and characterization studies
  • Plant Gene Expression Analysis

Manipal Academy of Higher Education
2025

Indian Institute of Pulses Research
2013-2024

Oil India (India)
2021

National Research Centre on Plant Biotechnology
2015

Indian Agricultural Research Institute
1997-2012

Rice (Oryza sativa) is a vital food crop and staple diet for most of the world's population. Poor dietary choices have had significant role in development type-2 diabetes population that relies on rice rice-starch-based foods. Hence, our study investigated vitro digestion glycemic indices certain indigenous cultivars factors influencing these indices. Cooking properties were estimated. Further, biochemical investgations such as amylose content, resistant starch content estimated using...

10.1038/s41598-025-85660-5 article EN cc-by Scientific Reports 2025-01-17

Rice blast resistant gene, Pi54 cloned from rice line, Tetep, is effective against diverse isolates of Magnaporthe oryzae. In this study, we prospected the allelic variants dominant resistance gene a set 92 lines to determine nucleotide diversity, pattern its molecular evolution, phylogenetic relationships and evolutionary dynamics, develop allele specific markers. High quality sequences were generated for homologs gene. Using comparative sequence analysis, InDels variable sizes in all...

10.3389/fpls.2015.00345 article EN cc-by Frontiers in Plant Science 2015-05-21

Domain swapping and generation of chimeric insecticidal crystal protein is an emerging area insect pest management. The lepidopteran pest, gram pod borer (Helicoverpa armigera H.) wreaks havoc to chickpea crop affecting production. Lepidopteran insects were reported be controlled by Bt (cryI) genes. We designed a plant codon optimized gene (cry1Aabc) using three domains from different cry1A genes (domains I, II, III cry1Aa, cry1Ab, cry1Ac, respectively) expressed it under the control...

10.3389/fpls.2017.01423 article EN cc-by Frontiers in Plant Science 2017-08-21

Abstract Background Chickpea ( Cicer arietinum L.) is the second most widely grown pulse and drought (limiting water) one of major constraints leading to about 40–50% yield losses annually. Dehydration responsive element binding proteins (DREBs) are important plant transcription factors that regulate expression many stress-inducible genes play a critical role in improving abiotic stress tolerance. Transgenic chickpea lines harbouring factor, Responsive Element-Binding protein 1A from...

10.1186/s12870-020-02815-4 article EN cc-by BMC Plant Biology 2021-01-11

Abstract Symbiotic nitrogen fixation (SNF) of transgenic grain legumes might be influenced either by the site transgene integration into host genome or due to constitutive expression transgenes and antibiotic-resistant marker genes. The present investigation confirmed proper nodulation five tested Bt -chickpea events (IPCa2, IPCa4, IPCT3, IPCT10, IPCT13) native Mesorhizobium under field environment. Quantitative variations for traits among were determined IPCT3 was found superior nodule...

10.1038/s41598-020-62199-1 article EN cc-by Scientific Reports 2020-03-25

Plant disease resistance (R) gene triggers defense mechanisms by detecting non-self or modified self-molecules (elicitors) which results in hypersensitive response.Plant possesses a large number of R-genes with diverse recognition specificities are activated response to variety microbial pathogens.Many studies have shown that variation is the result duplication followed divergence employing tandem segmental duplication, recombination, unequal crossing over, transposable element activity,...

10.16943/ptinsa/2014/v80i1/55088 article EN DELETED 2014-03-19

The gram pod borer is a major pest of chickpea, accounting for average annual yield losses to the tune 40-50%. VIP3Aa, class insecticidal protein with different receptor binding site in insect's midgut compared Bt-crystal protein, offers an alternative protection strategy against Lepidopteran insects. Here, we report evaluation genetically engineered chickpea lines harboring codon modified Vip3Aa (cmVip3Aa) insect pest, borer. synthetic modified, cmVip3Aa gene 2,370 bp was sub-cloned plant...

10.1371/journal.pone.0270011 article EN cc-by PLoS ONE 2022-06-24

Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, an average 31.4 fragments per combination. The rates obtained the...

10.1590/s0102-33062014000200007 article EN cc-by Acta Botanica Brasilica 2014-05-15

Sterility mosaic disease (SMD) is a major viral of pigeonpea in southern India. In the present study, simple sequence repeat (SSR) markers were employed to identify those genomic regions associated with resistance Bengaluru isolate SMD through bulked segregant analysis (BSA) approach. Eighty four F 6 recombinant inbred lines (RILs) derived from cross ICP 8863 (susceptible) x BRG 3 (resistant) used. A total 50 hypervariable specific SSR primers (AHSSR) screened for parental polymorphism...

10.5376/lgg.2016.07.0006 article EN Legume Genomics and Genetics 2016-01-01
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