A5067897852- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Lipid metabolism and biosynthesis
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- ATP Synthase and ATPases Research
- RNA Interference and Gene Delivery
- Drug Transport and Resistance Mechanisms
- Cellular transport and secretion
- Microbial Metabolic Engineering and Bioproduction
- Pancreatic function and diabetes
- Photoreceptor and optogenetics research
- Advanced Electron Microscopy Techniques and Applications
- Force Microscopy Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Receptor Mechanisms and Signaling
- Advanced Fluorescence Microscopy Techniques
- Endoplasmic Reticulum Stress and Disease
University of Groningen
2017-2021
Biotechnology Institute
2019-2020
Institute of Molecular Life Sciences
2020
Abstract One of the grand challenges in chemistry is construction functional out-of-equilibrium networks, which are typical living cells. Building such a system from molecular components requires control over formation and degradation interacting chemicals homeostasis internal physical-chemical conditions. The provision consumption ATP lies at heart this challenge. Here we report vitro pathway vesicles for sustained production that maintained away equilibrium by energy dissipation. We...
Yeast tolerates a low pH and high solvent concentrations. The permeability of the plasma membrane (PM) for small molecules is lateral diffusion proteins slow. These findings suggest degree lipid order, which raises question how function in such an environment. yeast PM segregated into Micro-Compartment-of-Can1 (MCC) Pma1 (MCP), have different compositions. We extracted from these microdomains via stoichiometric capture lipids styrene-maleic-acid-lipid-particles (SMALPs). purified...
(Micro)organisms are exposed to fluctuating environmental conditions, and adaptation stress is essential for survival. Increased osmolality (hypertonicity) causes outflow of water loss turgor dangerous if the cell not capable rapidly restoring its volume. The osmoregulatory adenosine triphosphate-binding cassette transporter OpuA restores volume by accumulating large amounts compatible solute. gated ionic strength inhibited second messenger cyclic-di-AMP, a molecule recently shown affect...
The mechanosensitive channels of large conductance (MscL) are bacterial membrane proteins that serve as last resort emergency release valves in case severe osmotic downshock. Sensing bilayer tension, MscL sensitive to changes the environment and are, therefore, an ideal test for exploring protein coupling. Here, we use high-throughput coarse-grained molecular dynamics simulations characterize gating kinetics different environments under influence alcohols. We performed over five hundred...
Membrane lipids act as solvents and functional cofactors for integral membrane proteins. The yeast plasma is unusual in that it may have a high lipid order, which coincides with low passive permeability small molecules slow lateral diffusion of Yet, proteins whose functions require altered conformation must flexibility within membranes. We determined the molecular composition located defined diameter model proteins, including APC-superfamily lysine transporter Lyp1. now use naturally...
Obtaining (dynamic) structure related information on proteins is key for understanding their function. Methods as single-molecule Förster Resonance Energy Transfer (smFRET) and Electron Paramagnetic (EPR) that measure distances between labeled residues to obtain dynamic rely selection of suitable residue pairs chemical modification. Selection amino acids, show sufficient distance changes upon activity the protein, can be a tedious process. Here we present an in silico approach makes use two...
Many proteins have a multimeric structure and are composed of two or more identical subunits. While this can be advantageous for the host organism, it challenge when targeting specific residues in biochemical analyses. In vitro splitting re-dimerization to circumvent problem is tedious process that requires stable proteins. We present an vivo approach transform homodimeric into apparent heterodimers, which then purified using two-step affinity-tag purification. This opens door both practical...
Abstract One of the grand challenges in chemistry is construction functional out-of-equilibrium networks, which are typical living cells. Building such a system from molecular components requires control over formation and degradation interacting chemicals homeostasis internal physical-chemical conditions. The provision consumption ATP lies at heart this challenge. We report vitro vesicles pathway for sustained production that maintained away equilibrium by energy dissipation. maintain...
Abstract The yeast plasma membrane is segregated into domains: the Micro-Compartment-of-Can1 (MCC) and Pma1 (MCP) have a different protein composition, but their lipid composition largely unknown. We extracted proteins residing in these microdomains via stoichiometric capture of lipids styrene-maleic-acid-lipid-particles (SMALPs). purified SMALPs by affinity chromatography quantitatively analyzed mass spectrometry role transporter function. found that phospholipid sterol concentrations are...
Abstract Membrane lipids act as solvents and functional cofactors for integral membrane proteins. The yeast plasma is unusual in that it may have a high lipid order, which coincides with low passive permeability small molecules slow lateral diffusion of Yet, proteins whose functions require altered conformation must flexibility within membranes. We determined the molecular composition located defined diameter model proteins, including APC-superfamily lysine transporter Lyp1. now use...
Abstract Yeast tolerates a low pH and high solvent concentrations. The permeability of the plasma membrane (PM) for small molecules is lateral diffusion proteins slow. These findings suggest degree lipid order, which raises question how function in such an environment. yeast PM segregated into Micro-Compartment-of-Can1 (MCC) Pma1 (MCP), have different compositions. We extracted from these microdomains via stoichiometric capture lipids styrene-maleic-acid-lipid-particles (SMALPs). purified...