A5036341651- Cancer therapeutics and mechanisms
- Synthesis and Biological Evaluation
- Synthesis and biological activity
- Reproductive Biology and Fertility
- PARP inhibition in cancer therapy
- Pregnancy and preeclampsia studies
- Liver physiology and pathology
- Invertebrate Immune Response Mechanisms
- DNA Repair Mechanisms
- Ferrocene Chemistry and Applications
- Chemotherapy-induced organ toxicity mitigation
- Synthesis and Reactions of Organic Compounds
- Aquaculture disease management and microbiota
- Cellular Mechanics and Interactions
- Chemical Reactions and Isotopes
- Synthesis and bioactivity of alkaloids
- DNA and Nucleic Acid Chemistry
- Cancer Genomics and Diagnostics
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Protein Kinase Regulation and GTPase Signaling
- Bioactive Compounds and Antitumor Agents
- Sulfur Compounds in Biology
- Glycosylation and Glycoproteins Research
Centre Hospitalier Universitaire de Montpellier
2025
Institut de Génétique Humaine
2025
Unité de Glycobiologie Structurale et Fonctionnelle
2020-2023
Université de Lille
2020-2023
Centre National de la Recherche Scientifique
2020-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022
Rationale: In the era of precision medicine, there is a growing need for rapid reliable ex vivo functional assays capable predicting treatment efficacy. One drug class that may particularly benefit from such BH3 mimetics. These small molecules antagonize anti-apoptotic proteins as BCL-2, MCL-1, or BCL-XL, on which cancer cells depend their survival. A assay known profiling was previously developed to measure those dependencies through use specific BH3-only peptides. variation this technique,...
Topoisomerases, targets of inhibitors used in chemotherapy, induce DNA breaks accumulation leading to cancer cell death. A newly synthesized copper(II) indenoisoquinoline complex WN197 exhibits a cytotoxic effect below 0.5 µM, on MDA-MB-231, HeLa, and HT-29 cells. At low doses, inhibits topoisomerase I. higher it IIα IIβ, displays intercalation properties. damage is detected by the presence γH2AX. The activation Damage Response (DDR) occurs through phosphorylation ATM/ATR, Chk1/2 kinases,...
Topoisomerases are interesting targets in cancer chemotherapy. Here, we describe the design and synthesis of a novel copper(II) indenoisoquinoline complex, WN198. The new organometallic compound exhibits cytotoxic effect on five adenocarcinoma cell lines (MCF-7, MDA-MB-231, HeLa, HT-29, DU-145) with lowest IC50 (0.37 ± 0.04 μM) for triple-negative MDA-MB-231 breast line. Below 5 µM, WN198 was ineffective non-tumorigenic epithelial MCF-10A cells Xenopus oocyte G2/M transition or embryonic...
Xenopus oocytes were used as cellular and molecular sentinels to assess the effects of a new class organometallic compounds called ferrocenyl dihydroquinolines that have been developed potential anti-cancer agents. One dihydroquinoline compound exerted deleterious on oocyte survival after 48 h incubation at 100 μM. Two had an inhibitory effect resumption progesterone induced meiosis, compared controls without groups. In these inhibited oocytes, no MPF (Cdk1/cyclin B) activity was detected by...
The role of hydrogen sulfide (H2S) is addressed in Xenopus laevis oocytes. Three enzymes involved H2S metabolism, cystathionine β-synthase, γ-lyase, and 3-mercaptopyruvate sulfurtransferase, were detected prophase I metaphase II-arrested oocytes drove an acceleration oocyte meiosis resumption when inhibited. Moreover, associated with a significant decrease endogenous H2S. On another hand, dose-dependent inhibition was obtained using the donor, NaHS (1 5 mM). impaired translation. did not...
The high‐affinity tyrosine kinase receptor MET plays a pivotal role in several facets of cell regulation. Although its mitogenic effect is well documented, some aspects connection patterns between signaling pathways involved cycle progression remain to be deciphered. We have used tractable heterologous expression system, the Xenopus oocyte, detect connections distinct cascades G2/M progression. Our results reveal that Src acts as an adapter via SH2 domain recruit 3‐phosphoinositide‐dependent...
Xenopus oocytes are encompassed by a layer of follicular cells that contribute to oocyte growth and meiosis in relation maturation. However, the effects interaction between surface on meiotic processes unclear. Here, we investigated cell function using signaling heterologous-expressing capabilities. We found deprotected from their surrounding expressing epidermal factor (EGF) receptor (EGFR) Grb7 adaptor undergo accelerated prophase I metaphase II progression upon stimulation EGF. This...