A5048463949- Gut microbiota and health
- Reproductive tract infections research
- Clostridium difficile and Clostridium perfringens research
- Inflammatory Bowel Disease
- Syphilis Diagnosis and Treatment
- Urinary Tract Infections Management
- Gastrointestinal motility and disorders
- Microscopic Colitis
- Probiotics and Fermented Foods
- Helicobacter pylori-related gastroenterology studies
- Gallbladder and Bile Duct Disorders
- Mycobacterium research and diagnosis
- Immune Response and Inflammation
- Biliary and Gastrointestinal Fistulas
- Pelvic floor disorders treatments
- Gastroesophageal reflux and treatments
- Soil and Environmental Studies
- Reproductive System and Pregnancy
- Nutrition and Health in Aging
- Congenital gastrointestinal and neural anomalies
- Barrier Structure and Function Studies
- Infant Health and Development
- Escherichia coli research studies
- Marine and environmental studies
- Diet and metabolism studies
Charité - Universitätsmedizin Berlin
2016-2025
Sechenov University
2017-2024
Freie Universität Berlin
2023
Humboldt-Universität zu Berlin
2002-2021
Humboldt State University
2001-2020
Leipzig University
2007-2017
Ghent University
2012
Laboratory of Molecular Genetics
2010
University of Vienna
1998
The composition and spatial organization of the mucosal flora in biopsy specimens from patients with inflammatory bowel disease (IBD; either Crohn's or ulcerative colitis), self-limiting colitis, irritable-bowel syndrome (IBS), healthy controls were investigated by using a broad range fluorescent bacterial group-specific rRNA-targeted oligonucleotide probes. Each group included 20 subjects. Ten who had IBD being treated antibiotics also studied. Use nonaqueous Carnoy fixative to preserve...
In Brief OBJECTIVE: Bacterial vaginosis is a common infectious disorder. Although known since ancient times, little progress has occurred in identifying causal factors. Our aims were to study the bacterial community structure and spatial organization of microbiota on epithelial surfaces vaginal biopsy specimens. METHODS: We investigated composition bacteria associated with epithelium specimens from 20 patients 40 normal premenopausal postmenopausal controls using broad range fluorescent...
Acute appendicitis is a local intestinal inflammation with unclear origin. The aim was to test whether bacteria in differ composition found caecal biopsies from healthy and disease controls.We investigated sections of 70 appendices using rRNA-based fluorescence situ hybridisation. Four hundred 400 faecal samples patients inflammatory bowel other conditions were used as controls. A set 73 group-specific bacterial probes applied for the study.The mucosal surface catarrhal showed characteristic...
<b>Aim:</b> To study the role of mucus in spatial separation intestinal bacteria from mucosa. <b>Patients and methods:</b> Mucus barrier characteristics were evaluated using histological material obtained by biopsy purged colon, colon prepared with enema untreated appendices fixed non-aqueous Carnoy solution. Bacteria fluorescence situ hybridization, bacterial 16S RNA probes related to periodic acid Schiff alcian blue stain. Biopsies controls (n = 20), patients self-limiting colitis (SLC; n...
AIM:To study the role of intestinal flora in inflammatory bowel disease (IBD). METHODS:The spatial organization was investigated normal mice and two models murine colitis using fluorescence situ hybridization. RESULTS:The small intestine nearly bacteriafree.The colonic organized three distinct compartments (crypt, interlaced, fecal), each with different bacterial compositions.Crypt bacteria were present cecum proximal colon.The fecal compartment composed homogeneously mixed groups that...
The intestinal microflora is important in the pathogenesis of inflammatory bowel disease (IBD). impact its spatial organization on health and unknown.We investigated sections paraffin-embedded punched fecal cylinders. Fluctuations distribution 11 bacterial groups were monitored healthy subjects (n = 32), patients with IBD 204), other gastrointestinal diseases 186) using fluorescence situ hybridization (FISH).The microbial structure differed Crohn's (CD), ulcerative colitis (UC), controls....
To assess whether the bacterial vaginosis biofilm extends into upper female genital tract.Endometrial samples obtained during curettage and fallopian tube salpingectomy were collected. Endometrial analyzed for presence of bacteria with fluorescence-in-situ-hybridisation (FISH) analysis probes targeting vaginosis-associated other bacteria.A structured polymicrobial Gardnerella vaginalis could be detected in part endometrial specimens. Women had a 50.0% (95% CI 24.0-76.0) risk presenting an...
Detergents and emulsifiers added to food may destroy the mucus barrier, which normally isolates bacteria from intestinal wall, lead chronic bowel inflammation in susceptible persons. We investigated influence of 2% carboxymethylcellulose (CMC) on biostructure microbiota IL-10 gene-deficient mice. Twenty 27-week-old mice received either CMC solution (n = 7) or water 6) orally for 3 weeks. Intestinal were using fluorescence situ hybridization paraffin-fixed sections intestine. CMC-treated...
Background: Colonic microbiome is thought to be involved in auto-immune multiple sclerosis (MS). Interactions between diet and the colonic MS are unknown. Methods: We compared composition of microbiota quantitatively 25 patients 14 healthy controls.Fluorescence situ hybridization (FISH) with 162 ribosomal RNA derived bacterial FISH probes was used. Ten received a ketogenic for 6 months. Changes concentrations 35 numerically substantial groups were monitored at baseline 2, 12, 23/24 weeks....
<i>Objective:</i> To study the incidence and distribution of adherent <i>Gardnerella vaginalis</i>. <i>Methods:</i> Bacteria to desquamated epithelial cells in urine were detected using fluorescence situ hybridization (FISH). Urine from patients with bacterial vaginosis (BV, n = 20), their partners (n 10) different control populations 344) including pregnant women partners, randomly selected hospitalized man, children as also healthy controls was...
Migration is an important virulence factor for intestinal bacteria. However, the role of bacterial mobility in penetration viscous mucus and their spatial organization within colon relatively unknown.Movements fecal bacteria were assessed gels varying agarose concentrations compared with patterns distribution observed colons from conventional Enterobacter cloacae-monoassociated mice. Bacteria visualized using fluorescence situ hybridization.Long curly moved best moderate viscosity gels,...