A5000326765- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Renal and related cancers
- Pluripotent Stem Cells Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Research and Splicing
- Reproductive System and Pregnancy
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Sepsis Diagnosis and Treatment
- RNA modifications and cancer
- Childhood Cancer Survivors' Quality of Life
- Neonatal Respiratory Health Research
- Nuclear Structure and Function
- Viral Infectious Diseases and Gene Expression in Insects
- Infant Development and Preterm Care
- Molecular Biology Techniques and Applications
- Cardiac Arrest and Resuscitation
- Respiratory Support and Mechanisms
- Erythrocyte Function and Pathophysiology
- Ovarian function and disorders
- Monoclonal and Polyclonal Antibodies Research
National Institutes of Health
2016-2025
National Institute of Diabetes and Digestive and Kidney Diseases
2016-2025
University of Birmingham
2025
University of New Mexico
2020-2024
Government of the United States of America
2023
University of Utah
2009-2022
Health and Human Development (2HD) Research Network
2022
Columbia University
2022
Institut thématique Génétique, génomique et bioinformatique
2022
Institute of Entomology
2021
Primordial follicles are formed perinatally in mammalian ovaries and at birth represent the lifetime complement of germ cells. With cyclic periodicity, cohorts enter into a growth phase that culminates ovulation mature eggs, but little is known about regulatory cascades govern these events. FIGalpha, transcription factor implicated postnatal oocyte-specific gene expression, detected as early embryonic day 13. Mouse lines lacking FIGalpha were established by targeted mutagenesis stem Although...
The mouse zona pellucida is composed of three glycoproteins (ZP1, ZP2, and ZP3), which ZP2 proteolytically cleaved after gamete fusion to prevent polyspermy. This cleavage associated with exocytosis cortical granules that are peripherally located subcellular organelles unique ovulated eggs. Based on the site ovastacin was selected as a candidate protease. Encoded by single-copy Astl gene, an oocyte-specific member astacin family metalloendoproteases. Using specific antiserum, detected in...
Severity of illness measures have long been used in pediatric critical care. The Pediatric Risk Mortality is a physiologically based score to quantify physiologic status, and when combined with other independent variables, it can compute expected mortality risk morbidity risk. Although the ranges for variables not changed, recent data collection improvements made adapt new practice patterns, minimize bias, reduce potential sources error. These include changing outcome hospital survival/death...
Functional status assessment methods are important as outcome measures for pediatric critical care studies.To investigate the relationships between 2 functional appropriate large-sample studies, Status Scale (FSS) and Pediatric Overall Performance Category Cerebral (POPC/PCPC) scales.Prospective cohort study with random patient selection at 7 sites 8 children's hospitals general/medical cardiac/cardiovascular intensive units (PICUs) in Collaborative Critical Care Research Network....
<h3>Importance</h3> Young febrile infants are at substantial risk of serious bacterial infections; however, the current culture-based diagnosis has limitations. Analysis host expression patterns (“RNA biosignatures”) in response to infections may provide an alternative diagnostic approach. <h3>Objective</h3> To assess whether RNA biosignatures can distinguish aged 60 days or younger with and without infections. <h3>Design, Setting, Participants</h3> Prospective observational study involving...
Mammalian oocytes synthesize and secrete a zona pellucida that surrounds the growing oocytes, ovulated eggs preimplantation embryos. The extracellular matrix is composed of three glycoproteins (ZP1, ZP2, ZP3) are involved in folliculogenesis, species-specific fertilization, passage early embryo down oviduct. We have established mouse line which Zp3 has been inactivated by homologous recombination with an insertional mutation. Neither transcripts nor ZP3 protein was detected female mice...
ABSTRACT The mouse zona pellucida is composed of three glycoproteins, ZP1, ZP2 and ZP3, encoded by single-copy genes whose expression temporally spatially restricted to oocytes. All proteins are required for the formation extracellular matrix female mice with a single disrupted gene lack infertile. An E-box (CANNTG), located approximately 200 bp upstream transcription start sites Zp1, Zp2 Zp3, forms protein-DNA complex present in oocytes and, much lesser extent, testes. It has been...
ABSTRACT The mammalian zona pellucida is an extracellular matrix that surrounds growing oocytes, ovulated eggs and early embryos. mouse composed of three sulfated glycoproteins: ZP1, ZP2 ZP3. Each critically involved in fertilization, the postfertilization block to polyspermy protection preimplantation embryo. We have previously isolated cDNAs encoding ZP3 now report isolation a full-length cDNA ZP1. Mouse ZP1 623 amino acid polypeptide chain with signal peptide carboxyl terminal...
Abstract All vertebrates have an egg shell that surrounds ovulated eggs and plays critical roles in gamete recognition. This extracellular matrix is known as the zona pellucida eutherian mammals consists of three glycoproteins, ZP1, ZP2 ZP3 mouse. To investigate role ZP1 fertilization early development, we used targeted mutagenesis embryonic stem cells to create mouse lines (Zp1tm/tm) lacking ZP1. Although a composed was formed around growing Zp1tm/tm oocytes, more loosely organized than...
ABSTRACT All vertebrate eggs are surrounded by an extracellular matrix. This matrix is known as the zona pellucida in mammals and critically important for survival of growing oocytes, successful fertilization passage early embryos through oviduct. The mouse composed three glycoproteins (ZP1, ZP2 ZP3), each encoded a single copy gene. Using targeted mutagenesis embryonic stem cells, Zp2-null lines have been established. ZP1 ZP3 proteins continue to be synthesized form thin follicles that not...
THE term "molecular disease" was coined almost 30 years ago by Linus Pauling and his associates to describe their analysis of the abnormality in sickle-cell anemia.1 Since that time tremendous progress has been made understanding structure function this mutant hemoglobin molecule conditions under which it aggregates when deoxygenated form a gel. Indeed, last decade, particularly few years, there an explosive growth knowledge gel sickle mechanism its formation. The exact relations among...
Objective: Communicating bad news about a child's illness is difficult task commonly faced by intensive care physicians. Greater understanding of parents' scope experiences with during their hospitalization will help physicians communicate more effectively. Our objective to describe perceptions conversations regarding terminal and death in the pediatric unit (PICU). Design: A secondary analysis qualitative interview study. Setting: Six children's hospitals National Institute Child Health...
The zona pellucida surrounding mouse oocytes is an extracellular matrix composed of three sulfated glycoproteins, ZP1, ZP2, and ZP3. It has been demonstrated that a monoclonal antibody to ZP3 injected into female mice inhibits fertilization by binding the blocking sperm penetration. A complementary DNA encoding was randomly cleaved 200- 1000-base pair fragments were cloned expression vector lambda gt11. This epitope library screened with aforementioned contraceptive antibody, positive clones...
The third component of human complement, hemoglobin Az, and IgG have been radiolabeled with tritium to specific activities 179, 103, 89 Ci/mmol, respectively, by reductive methylation.The labeling procedure is mild, requiring only brief exposure formaldehyde (10 12 m ~) tritiated sodium borohydride (3 5 , for the a amino groups NHz-terminal residues €-amino lysyl residues."he extent modification each protein ranged between 16% at available based on stoichiometric reactivity 2 mol...
The zona pellucida is an extracellular glycocalyx that surrounds the growing oocyte and mediates specific biological functions essential to early mammalian development.We have isolated by a novel technique intact zonae pellucidae from murine follicle culture system which mimics in vivo granulosa cell-oocyte interactions.Using radioactive precursors we demonstrated all three proteins, ZP-1,ZP-2, ZP-3, are sulfated glycoproteins.Incubation of with tunicamycin (0.5 pg/ml), antibiotic blocks...
We describe a novel experimental system in mice for the study of ovarian autoimmune disease, condition encountered women with premature failure. The disease is induced B6AF1 by 15-amino acid peptide (Cys-Ser-Asn-Ser-Ser-Ser-Ser-Gln-Phe-Gln-Ile-His-Gly-Pro-Arg) from mouse ZP3, sperm-binding component zona pellucida that surrounds growing and mature oocytes. Whereas induces both T cell antibody responses, adoptive transfer CD4+ lines derived affected animals causes oophoritis without...
At fertilization, mouse sperm bind to the zona pellucida (which consists of glycoproteins ZP1, ZP2, and ZP3) that surrounds eggs. A ZP2 cleavage model gamete recognition requires intact a glycan release postulates glycans are ligands for sperm. These two models were tested by replacing endogenous protein with cannot be cleaved (Zp2(Mut)) or ZP3 lacking implicated O (Zp3(Mut)). Sperm bound two-cell Zp2(Mut) embryos despite fertilization cortical granule exocytosis. Contrary prediction,...
The human zona pellucida, composed of three glycoproteins (ZP1, ZP2, and ZP3), forms an extracellular matrix that surrounds ovulated eggs mediates species-specific fertilization. genes code for at least two the proteins (ZP2 ZP3) cross-hybridize with other mammalian DNA. recently characterized mouse sperm receptor gene (Zp-3) was used to isolate its homolog. homolog spans approximately 18.3 kilobase pairs (kbp) (compared 8.6 kbp gene) contains eight exons, sizes which are strictly conserved...