A5022901741- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Plant Virus Research Studies
- RNA and protein synthesis mechanisms
- Microbial Community Ecology and Physiology
- RNA modifications and cancer
Latvian Biomedical Research and Study Centre
2017-2025
Endolysins—enzymes produced by tailed bacteriophages to degrade bacterial cell walls—have traditionally been classified as canonical or signal‐anchor‐release (SAR) endolysins. However, analysis of expanding viral (meta)genomic data has revealed a third class, which we designate C‐terminal anchor (CTA) These enzymes feature an N‐terminal enzymatic domain, transmembrane and typically lack signal sequences, distinguishing them from SAR CTA endolysins span all known activities exhibit diverse...
Abstract Endolysins are bacteriophage-encoded peptidoglycan-degrading enzymes with potential applications for treatment of multidrug-resistant bacterial infections. Hafnia phage Enc34 encodes an unusual endolysin N-terminal enzymatically active domain and a C-terminal transmembrane domain. The catalytic the belongs to conserved protein family PHA02564 which has no recognizable sequence similarity other known types. Turbidity reduction assays indicate that enzyme is against peptidoglycan from...
Abstract Modern DNA sequencing capabilities have led to the discovery of a large number new bacteriophage genomes, which are rich source novel proteins with an unidentified biological role. The genome Enterobacter cancerogenus Enc34 contains several unknown function that nevertheless conserved among distantly related phages. Here, we report crystal structure replication protein ORF6 domain DUF2815. Despite low (~15%) sequence identity, structurally resembles gene 2.5 from T7, and likewise is...
ABSTRACT In our 2012 genome announcement (J Virol 86:11403–11404, 2012, https://doi.org/10.1128/JVI.01954-12 ), we initially identified the host bacterium of bacteriophage Enc34 as Enterobacter cancerogenus using biochemical tests. However, later in-house DNA sequencing revealed that true is a strain Hafnia alvei . Capitalizing on new DNA-sequencing capabilities, also refined genomic termini Enc34, confirming 60,496-bp with 12-nucleotide 5′ cohesive ends. IMPORTANCE Our correction reflects...