A5044165924- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Platelet Disorders and Treatments
- Caveolin-1 and cellular processes
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- HER2/EGFR in Cancer Research
- Biochemical and Structural Characterization
- Protease and Inhibitor Mechanisms
- Immunotherapy and Immune Responses
- Cellular Mechanics and Interactions
- Chronic Lymphocytic Leukemia Research
- Signaling Pathways in Disease
- T-cell and B-cell Immunology
- Cancer, Hypoxia, and Metabolism
- Advanced Proteomics Techniques and Applications
- Immune Response and Inflammation
- Inflammatory mediators and NSAID effects
- Galectins and Cancer Biology
- Biotin and Related Studies
- Proteoglycans and glycosaminoglycans research
- S100 Proteins and Annexins
- Phagocytosis and Immune Regulation
- Estrogen and related hormone effects
- Lipid Membrane Structure and Behavior
Dana-Farber Cancer Institute
2009-2022
Harvard University
2005-2017
Boston University
1982-2012
University of Massachusetts Boston
2010
Harvard University Press
1980-2008
AIDS United
2008
Dana-Farber Brigham Cancer Center
2003
Brigham and Women's Hospital
1993-2003
Massachusetts Institute of Technology
2003
Howard Hughes Medical Institute
1998
Selectins have previously been shown to tether a flowing leukocyte vessel wall and mediate rolling. Here, we report that an intergrin, VLA-4, can also support tethering Blood T lymphocytes alpha 4 integrin-transfected cells in shear flow, then roll, through binding of the intergrin VLA-4 purified VCAM-1 on flow chamber. transfectants showed similar rolling TNF-stimulated endothelium. Tethering efficiency, velocity, resistance detachment are related density. did not occur ICAM-1, fibronectin,...
Cell adhesion to extracellular matrices is mediated by a set of heterodimeric cell surface receptors called integrins that might be the subject regulation growth and differentiation factors. We have examined effect transforming factor-beta 1 (TGF-beta 1) on expression very late antigens or alpha beta group in human lines. The six known members this family share common subunit but distinct subunits confer selective affinity toward type I collagen, fibronectin, laminin, other as yet unknown...
The family of human cell surface heterodimers which includes VLA-1 and VLA-2 is now shown to include three additional heterodimers, here called VLA-3, VLA-4, VLA-5.Each these separate VLA structures composed a distinct (Y subunit (Mr 110,000-200,000 nonreduced) noncovalently associated with common ,f3 110,000 nonreduced).Chemical cross-linking experiments provided evidence that each complex exists predominantly as 1 : l a@ heterodimer.The proteins are widely distributed, one or more the...
High levels of NaCN (20 to 250 mM) were required inhibit cyclooxygenase catalysis and cause extended lag periods (up 1.6 min), whereas CO failed catalysis.T h i s inhibition was easily overcome by endogenous or exogenous hydroperoxides.Added hydroperoxides acted eliminate without undergoing net conversion other chemical species.In addition, experiments with glutathione peroxidase showed that essential not only in the early phases, but throughout catalysis.In spectrophotometric experiments,...
The capability of the integrin VLA-3 to function as a receptor for collagen (Coll), laminin (Lm), and fibronectin (Fn) was addressed using both whole cell adhesion assays ligand affinity columns. Analysis VLA-3-mediated facilitated by use small lung carcinoma line (NCI-H69), which expresses but few other integrins. While interaction with Fn often low or undetectable in cells having VLA-5, NCI-H69 readily attached VLA-3-dependent manner. Both Arg-Gly-Asp (RGD) peptide inhibition studies,...
The fatty acid cyclooxygenase (ec 1.14.99.1) that produces the prostaglandin and thromboxane precursor, 15-hydroperoxy-9 alpha, 11 alpha-peroxidoprosta-5, 13-dienoic (PGG2), has been purified from sheep vesicular glands to a specific activity of 46,000 units/mg protein by combining detergent solubilization, (NH4)SO4 fractionation, chromatography on DEAE-cellulose Flurbiprofen-Sepharose, isoelectric focusing, gel filtration. final enzyme preparation exhibited only one band 70,000 molecular...
A glycoprotein complex of 210,000 and 130,000 m.w., found on mitogen or alloantigen-stimulated human T cells not other hematopoietic cells, has been defined by a monoclonal antibody (Mab). The components this are subset larger family proteins (210,000, 165,000 m.w.) second Mab. In panel cell lines types, only activated (including the line HUT-102) express 210,000/130,000 these also IL 2 receptor, characteristic marker for cells. m.w. (reactive with Mab TS2/7) is present all long-term...
The integrin heterodimer VLA-2, previously known as a collagen receptor, is now shown also to be laminin receptor. Adhesion of the human melanoma cell line LOX was inhibited by anti-VLA alpha 2 antibodies. Because VLA-2-mediated attachment not digestion with collagenase, contamination factor. In addition, VLA-2 from cells bound immobilized laminin, and binding disrupted EDTA but Arg-Gly-Asp (RGD) peptides. VLA-3 laminin-Sepharose, although less avidly than VLA-2. Thus, at least four separate...
Hematopoietic stem cell interaction with elements of the underlying stroma is essential for sustained normal hematopoiesis. Here we have determined that adhesion receptors in integrin family play a role promoting human hematopoietic cells to cultured marrow stromal cells. Enriched CD34hi progenitor expressed VLA-4, VLA-5, and at least one or more beta 2 integrins. Homogeneous monolayers capable supporting proliferation cocultivated VCAM-1 fibronectin (ligands VLA-4 VLA-5) as well ICAM-1...
VLA-2 (also called gpIa/IIa on platelets) is a collagen receptor with unique alpha subunit and beta common to other adhesion receptors in the VLA/integrin family. Multiple cDNA clones for human 2 have been selected from lambda gtll library by specific antibody screening. The 5,374-bp nucleotide sequence encoded 1,181 amino acids, including signal peptide of 29 acids followed long extracellular domain (1,103 acids), transmembrane domain, short cytoplasmic segment (22 acids). Direct sequencing...
A monoclonal antibody (B-5G10) was produced which specifically recognizes the Mr 150,000/130,000 VLA-4 complex on surface of human cells. Cross-linking studies indicated that 150,000 alpha 4 subunit is in noncovalent 1:1 association with 130,000 VLA beta subunit. In absence cross-linking, easily dissociated, especially Nonidet P-40 detergent, or at elevated pH (above 8.0). Studies dissociated subunits showed B-5G10 an epitope VLA-4, whereas immunologically identical to common all...