A5017827359- Viral Infectious Diseases and Gene Expression in Insects
- 3D Printing in Biomedical Research
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Additive Manufacturing and 3D Printing Technologies
- Cellular Mechanics and Interactions
- Transgenic Plants and Applications
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Electrospun Nanofibers in Biomedical Applications
- Virus-based gene therapy research
- Hydrogels: synthesis, properties, applications
- Connective tissue disorders research
- Blood properties and coagulation
- Bone Tissue Engineering Materials
- Monoclonal and Polyclonal Antibodies Research
- Supramolecular Self-Assembly in Materials
- Biochemical and Molecular Research
- Proteins in Food Systems
- Innovative Teaching Methods
- Genetics, Bioinformatics, and Biomedical Research
- Microbial Metabolic Engineering and Bioproduction
- Biomedical and Engineering Education
- Skin and Cellular Biology Research
- Innovative Microfluidic and Catalytic Techniques Innovation
University of Washington
2023-2025
California Polytechnic State University
2018-2020
Relating the macroscopic properties of protein-based materials to their underlying component microstructure is an outstanding challenge. Here, we exploit computational design specify size, flexibility, and valency de novo protein building blocks, as well interaction dynamics between them, investigate how molecular parameters govern viscoelasticity resultant hydrogels. We construct gel systems from pairs symmetric homo-oligomers, each comprising 2, 5, 24, or 120 individual components, that...
Over the last 50 years, Cell-Free Protein Synthesis (CFPS) has emerged as a powerful technology to harness transcriptional and translational capacity of cells within test tube. By obviating need maintain viability cell, by eliminating cellular barrier, CFPS been foundational emerging applications in biomanufacturing traditionally challenging proteins, well rapid prototyping for metabolic engineering, functional genomics. Our methods implementing an E. coli-based platform allow new users...
Abstract While direct cell transplantation holds great promise in treating many debilitating diseases, poor survival and engraftment following injection have limited effective clinical translation. Though injectable biomaterials offer protection against membrane‐damaging extensional flow supply a supportive 3D environment vivo that ultimately improves retention therapeutic costs, most are created from synthetic or naturally harvested polymers immunogenic and/or chemically ill‐defined. This...
Cell-free protein synthesis (CFPS) is a platform biotechnology that enables breadth of applications. However, field applications remain limited due to the poor shelf-stability aqueous cell extracts required for CFPS. Lyophilization E. coli improves shelf life but remains insufficient extended storage at room temperature. To address this limitation, we mapped chemical space ten low-cost additives with four distinct mechanisms action in combinatorial manner identify formulations capable...
Teaching the processes of transcription and translation is challenging due to intangibility these concepts a lack instructional, laboratory-based, active learning modules. Harnessing genetic code in vitro with cell-free protein synthesis (CFPS) provides an open platform that allows for direct manipulation reaction conditions biological machinery enable inquiry-based learning. Here, we report our efforts transform research-based CFPS biotechnology into hands-on module called "Genetic Code...
Abstract Hydrogel biomaterials offer great promise for 3D cell culture and therapeutic delivery. Despite many successes, challenges persist in that gels formed from natural proteins are only marginally tunable while those derived synthetic polymers lack intrinsic bioinstructivity. Towards the creation of with both excellent biocompatibility customizability, recombinant protein-based hydrogels have emerged as molecularly defined user-programmable platforms mimic proteinaceous nature...
Over the last 50 years, Cell-Free Protein Synthesis (CFPS) has emerged as a powerful technology to harness transcriptional and translational capacity of cells within test tube. By obviating need maintain viability cell, by eliminating cellular barrier, CFPS been foundational emerging applications in biomanufacturing traditionally challenging proteins, well rapid prototyping for metabolic engineering, functional genomics. Our methods implementing an E. coli-based platform allow new users...
Cell‐Free Protein Synthesis (CFPS) is a platform biotechnology that enables breadth of applications, from expressing traditionally intractable proteins to metabolic engineering and point‐of‐care diagnostics. However, field applications have remained limited due the poor shelf stability aqueous E. coli cell‐free extracts, which contain complex mixture cellular machinery. Lyophilization extract improves shelf‐life, but remains insufficient for maintaining productivity over extended storage...
Abstract Material- and cell-based technologies such as engineered tissues hold great promise human therapies. Yet, the development of many these becomes stalled at stage pre-clinical animal studies due to tedious low-throughput nature in vivo implantation experiments. We introduce a ‘plug play’ screening array platform called Highly Parallel Tissue Grafting (HPTG). HPTG enables parallelized 43 three-dimensional microtissues within single 3D printed device. Using HPTG, we screen microtissue...
Relating the macroscopic properties of protein-based materials to their underlying component microstructure is an outstanding challenge. Here, we exploit computational design specify size, flexibility, and valency
Hands‐on learning of transcription and translation remains limited due to a lack classroom modules for early‐career students. Currently, classrooms implement animations, simulations, games, bacterial transformation/protein expression teach the genetic code. The cell wall poses primary barrier students directly observe manipulate intracellular environment, limiting inquiry‐based We have overcome this limit by levering in vitro. open nature cell‐free protein synthesis (CFPS) is achieved...
E. coli‐based cell‐free protein synthesis (CFPS) is a flexible platform technology for on‐demand production that allows users to express traditionally intractable proteins, perform high‐throughput screening, and, with augmentation, supports applications such as metabolic engineering and genetic code expansion. The broad utility of the CFPS arises from elimination cellular membrane capture transcription translation machinery in vitro. This obviates need living cells creates an open system...