A5029011338- Knee injuries and reconstruction techniques
- Total Knee Arthroplasty Outcomes
- Osteoarthritis Treatment and Mechanisms
- Tendon Structure and Treatment
- Sports injuries and prevention
- Orthopaedic implants and arthroplasty
- Lower Extremity Biomechanics and Pathologies
- Shoulder Injury and Treatment
- Mesenchymal stem cell research
- Orthopedic Surgery and Rehabilitation
- Periodontal Regeneration and Treatments
- Hip disorders and treatments
- Foot and Ankle Surgery
- Sarcoma Diagnosis and Treatment
- Bone and Joint Diseases
- Nerve injury and regeneration
- Spine and Intervertebral Disc Pathology
- Shoulder and Clavicle Injuries
- Cervical and Thoracic Myelopathy
- Nerve Injury and Rehabilitation
- Peripheral Nerve Disorders
- Musculoskeletal synovial abnormalities and treatments
- Bone fractures and treatments
- Bone Tumor Diagnosis and Treatments
- Tissue Engineering and Regenerative Medicine
Hiroshima University
2014-2024
Hiroshima University Hospital
2006-2019
Higashihiroshima Medical Center
2018-2019
Statistical Research (United States)
2018
Hiroshima University of Economics
2015-2018
The Japanese Society of Gastroenterological Surgery
2018
Hiroshima Prefectural Rehabilitation Center
2018
Hiroshima City University
2017
Matsuyama Red Cross Hospital
2015
Hiroshima Prefectural Hospital
2003-2015
Abstract Objective Several microRNA, which are ∼22‐nucleotide noncoding RNAs, exhibit tissue‐specific or developmental stage–specific expression patterns and associated with human diseases. The objective of this study was to identify the pattern microRNA‐146 (miR‐146) in synovial tissue from patients rheumatoid arthritis (RA). Methods miR‐146 5 RA, osteoarthritis (OA), 1 normal subject analyzed by quantitative reverse transcription–polymerase chain reaction (RT‐PCR) situ hybridization...
Mesenchymal stem cell (MSC) transplantation is used for treatment of many diseases. The paracrine role MSCs in tissue regeneration attracting particular attention. We investigate the MSC exosomes skeletal muscle regeneration. promote myogenesis and angiogenesis vitro, an vivo model injury. Although had low concentrations muscle-repair-related cytokines, a number repair-related miRNAs were identified. This study suggests that MSC-derived by enhancing angiogenesis, which at least part mediated...
Abstract Objective A role of microRNA, which are ∼22‐nucleotide noncoding RNAs, has recently been recognized in human diseases. The objective this study was to identify the expression pattern microRNA‐146a (miR‐146a) cartilage from patients with osteoarthritis (OA). Methods miR‐146a 15 OA analyzed by quantitative reverse transcription–polymerase chain reaction (RT‐PCR) and situ hybridization. Induction cultures normal articular chondrocytes following stimulation interleukin‐1β (IL‐1β)...
Abstract Paracrine signaling by bone-marrow-derived mesenchymal stem cells (MSCs) plays a major role in tissue repair. Although the production of regulatory cytokines MSC transplantation is critical modulator regeneration, we focused on exosomes, which are extracellular vesicles that contain proteins and nucleic acids, as novel additional cell-to-cell communication regeneration. To address this, used radiologic imaging, histological examination, immunohistochemical analysis to evaluate...
A total of 108 patients with unilateral instability the knee, associated rupture anterior cruciate ligament, was prospectively randomised for arthroscopic single- or double-bundle reconstruction ligament using hamstring tendons. The same post-operative rehabilitation protocol used all. were followed up a mean 32 months (24 to 36). We measured laxity and joint position sense at different angles flexion knee determine whether both bundles in contributed stability proprioception. No significant...
MicroRNA, a class of noncoding RNA, play role in human diseases. MicroRNA-146a (miR-146a) is negative regulator immune and inflammatory responses, strongly expressed rheumatoid arthritis (RA) synovium peripheral blood mononuclear cells (PBMCs). This study was undertaken to examine whether miR-146a expression inhibits osteoclastogenesis, administration prevents joint destruction mice with collagen-induced (CIA).PBMCs from healthy volunteers were isolated seeded culture plates. The following...
Osteoarthritis (OA) is a whole joint disease, and characterized by progressive degradation of articular cartilage, synovial hyperplasia, bone remodeling angiogenesis in various tissues. Exosomes are type microvesicles (MVs) that may play role tissue-tissue cell-cell communication homeostasis diseases. We hypothesized exosomes function novel regulatory network contributes to OA pathogenesis examined the among tissue cells.Human fibroblasts (SFB) chondrocytes were obtained from normal knee...
We investigated the clinical, arthroscopic and biomechanical outcome of transplanting autologous chondrocytes, cultured in atelocollagen gel, for treatment full-thickness defects cartilage 28 knees (26 patients) over a minimum period 25 months. Transplantation eliminated locking knee reduced pain swelling all patients. The mean Lysholm score improved significantly. Arthroscopic assessment indicated that 26 (93%) had good or excellent outcome. There were few adverse features, except marked...
AbstractWe have investigated the correlation between number of mechanoreceptors in anterior cruciate ligament (ACL) remnants and joint position sense just before an ACL reconstruction 29 patients. The was evaluated histologically, using Gairns gold chloride method. Mechanoreceptors were also found patients who had a long interval injury operation. A test done within 3 days surgery. We positive accuracy sense, suggesting that proprioceptive function is related to mechanoreceptors. Therefore,...
The effects of hyaluronic acid (HA) on the proliferation and chondroitin sulfate (CS) synthesis chondrocytes embedded in collagen gels were examined. Articular cartilage was isolated from humerus, femur, tibia 21 10-week-old Japanese white rabbits. Chondrocytes by collagenase digestion type I cultured Dulbecco's modified Eagle's medium (DMEM) with various doses HA for 4 weeks. Histological biochemical evaluations performed at postculture weeks 1, 2, 3, 4. For evaluations, isomers such as...
In 52 patients we compared the accuracy of standard anteroposterior (AP) radiography, mortise radiography and MRI with arthroscopy ankle for diagnosis a tear tibiofibular syndesmosis. comparison arthroscopy, sensitivity, specificity were 44.1%, 100% 63.5% AP 58.3%, 71.2% radiography. For they 100%, 93.1% 96.2% anterior inferior ligament posterior ligament. Standard did not always provide correct diagnosis. was useful although there two-false positive cases. We suggest that is indispensable accurate
We investigated the clinical outcome of a reconstructive procedure medial patellofemoral ligament for treatment habitual or recurrent dislocation patella in four children (6 knees), with minimum follow-up years. The technique involves transfer tendon semitendinosus to using posterior one-third femoral insertion collateral as pulley. There was no recurrence after surgery. mean Kujala score at 96.3 points. Radiological assessment showed that congruence angle, tilt angle and lateral shift radio...
To compare the use of magnetic resonance (MR) imaging with arthroscopy for diagnosis tibiofibular syndesmotic injury.This study involved 58 patients who had ankle sprains or distal fibular fractures and underwent surgery. All were examined MR injury. When revealed ligament discontinuity (criterion 1) either a wavy curved contour nonvisualization 2), injury was considered to be disruption. After imaging, performed in all definitive disruption.Arthroscopic findings showed anteroinferior...
Abstract Background Interleukin (IL)-17 is an important factor in rheumatoid arthritis (RA) pathogenesis. MicroRNA (miRNA)s are a family of non coding RNAs and associated with human diseases including RA. The purpose this study to identify the miRNAs differentiation IL-17 producing cells, analyze their expression pattern peripheral blood mononuclear cells (PBMC) synovium from RA patients. Methods were expanded CD4+T cell. MiRNA microarray was performed cells. Quantitative polymerase chain...
Abstract MicroRNA (miRNA)s are a class of non‐coding RNAs that regulate gene expression post‐transcriptionally. Muscle‐specific miRNA, miRNA (miR)‐1, miR‐133 and miR‐206 play crucial role in the regulation muscle development homeostasis. Muscle injuries common muscloskeletal disorder, most effective treatment has not been established yet. The purpose this study was to demonstrate local injection double‐stranded (ds) miR‐1, 206 can accelerate regeneration rat skeletal injury model. After...
We examined whether somatosensory evoked potentials (SEPs) were detectable after direct electrical stimulation of injured, reconstructed and normal anterior cruciate ligaments (ACL) during arthroscopy under general anaesthesia. investigated the position sense knee before reconstruction correlation between SEP instability. found SEPs in all which had been with autogenous semitendinosus gracilis tendons over past 18 months as well cases group. The was only 15 out 32 injured group, although...
Background: Consistency of arthroscopic evaluation and documentation in meniscal tears between investigators is essential to the validity multicenter studies. A group experts developed a classification that may be used internationally. Hypothesis: The International Society Arthroscopy, Knee Surgery Orthopaedic Sports Medicine (ISAKOS) provides sufficient interobserver reliability for pooling data from international clinical trials designed evaluate outcomes treatment tears. Study Design:...
Considerable effort has focused on the development of selective protein farnesyl transferase (FTase) and geranylgeranyl (GGTase) inhibitors as cancer chemotherapeutics. Here, we report a new strategy for anticancer therapeutic agents involving inhibition diphosphate synthase (FPPS) (GGPPS), two enzymes upstream FTase GGTase, by lipophilic bisphosphonates. Due to dual site targeting decreased polarity, compounds have activities far greater than do current bisphosphonate drugs in inhibiting...