A5073401621- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Click Chemistry and Applications
- Viral Infections and Outbreaks Research
- T-cell and Retrovirus Studies
- Plant Virus Research Studies
- Animal Virus Infections Studies
- Poxvirus research and outbreaks
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
- PARP inhibition in cancer therapy
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2019-2024
Consejo Superior de Investigaciones Científicas
2022
African swine fever virus (ASFV) infectious cycle starts with the viral adsorption and entry into host cell. Then, is internalized via clathrin/dynamin mediated endocytosis macropinocytosis. Similar to other viruses, ASF virion then incorporated endocytic pathway. While endosomal maturation entails luminal acidification, decrease in pH acts on multilayer structure of dissolving outer capsid. Upon decapsidation, inner membrane exposed interact limiting late endosome for fusion. Viral fusion...
Niemann-Pick type C1 (NPC1) receptor is an endosomal membrane protein that regulates intracellular cholesterol traffic. This has been shown to play important role for several viruses. It reported SARS-CoV-2 enters the cell through plasma fusion and/or entry upon availability of proteases. However, whole process not fully understood yet and additional viral/host factors might be required viral subsequent replication. Here, we report a novel interaction between nucleoprotein (N) transporter...
African Swine Fever virus (ASFV) causes one of the most relevant emerging diseases affecting swine, now extended through three continents. The has a large coding capacity to deploy an arsenal molecules antagonizing host functions. In present work, we have studied only known E2 viral-conjugating enzyme, UBCv1 that is encoded by I215L gene ASFV. was expressed as early expression protein accumulates throughout course infection. This versatile protein, bound several types polyubiquitin chains...
African swine fever virus (ASFV) encodes more than 150 proteins, most of them unknown function. We used a high-throughput proteomic analysis to elucidate the interactome four ASFV which potentially mediate critical step infection cycle, fusion and endosomal exit virions. Using affinity purification mass spectrometry, we were able identify potential interacting partners for those proteins P34, E199L, MGF360-15R E248R. Representative molecular pathways these intracellular Golgi vesicle...
Microtubule targeting agents (MTAs) have been exploited mainly as anti-cancer drugs because of their impact on cellular division and angiogenesis. Additionally, microtubules (MTs) are key structures for intracellular transport, which is frequently hijacked during viral infection. We analyzed the antiviral activity clinically used MTAs in infection DNA RNA viruses, including SARS-CoV-2, to find that MT destabilizer show a higher than stabilizers infections tested, FDA-approved anti-helminthic...
African swine fever virus (ASFV) belongs to the family of Asfarviridae, part group nucleocytoplasmic large DNA viruses (NCLDV). Little is known about internalization ASFV in host cell and fusion membrane events that take place at early stages infection. Poxviruses, also members NCLDV represented by vaccinia (VACV), are large, enveloped, double-stranded viruses. Poxviruses were considered unique having an elaborate entry-fusion complex (EFC) composed 11 highly conserved proteins integrated...
ABSTRACT African swine fever virus (ASFV) infectious cycle starts with the viral adsorption and entry into host cell. The is internalized via clathrin/dynamin mediated endocytosis macropinocytosis. As several other viruses, ASF virion then incorporated endocytic pathway. Endosomal maturation entails luminal acidification lowering of pH acting on multi-layered structure dissolves outer capsid. Upon decapsidation, inner membrane exposed to interact limiting late endosome for fusion. Egress...
Abstract Niemann-Pick type C1 (NPC1) receptor is an endosomal membrane protein that regulates intracellular cholesterol trafficking, which crucial in the Ebola virus (EBOV) cycle. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters cell by binding of viral spike (S) to ACE2 receptor. This requires S-protein processing either surface transmembrane serine protease TMPRSS2 for plasma fusion or cathepsin L entry. Additional host factors are required at endosomes. Here, we...