A5043005036- CRISPR and Genetic Engineering
- Microbial Metabolic Engineering and Bioproduction
- Gene Regulatory Network Analysis
- Biofuel production and bioconversion
- MicroRNA in disease regulation
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Amino Acid Enzymes and Metabolism
- Anaerobic Digestion and Biogas Production
- Legume Nitrogen Fixing Symbiosis
- Bacterial Genetics and Biotechnology
- Molecular Communication and Nanonetworks
- Nematode management and characterization studies
- Evolutionary Algorithms and Applications
- RNA and protein synthesis mechanisms
- Biopolymer Synthesis and Applications
- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Advanced Memory and Neural Computing
University of Pavia
2019-2024
Synthetic circuit design is crucial for engineering microbes that process environmental cues and provide biologically relevant outputs. To reliably scale-up complexity, the availability of parts toolkits central. Streptococcus pyogenes (sp)-derived CRISPR interference/dead-Cas9 (CRISPRi/spdCas9) widely adopted implementing programmable regulations in synthetic circuits, alternative CRISPRi systems will further expand our orthogonal components. Here, we showcase potential using engineered...
MicroRNAs, highly-conserved small RNAs, act as key regulators of many biological functions in both plants and animals by post-transcriptionally regulating gene expression through interactions with their target mRNAs. The microRNA research is a dynamic field, which new unconventional aspects are emerging alongside well-established roles development stress adaptation. A recent hypothesis states that miRNAs can be transferred from one species to another potentially genes across distant species....
CRISPR and CRISPRi systems have revolutionized our biological engineering capabilities by enabling the editing regulation of virtually any gene, via customization single guide RNA (sgRNA) sequences. modules can work as programmable logic inverters, in which dCas9-sgRNA complex represses a target transcriptional unit. They been successfully used bacterial synthetic biology to engineer information processing tasks, an alternative traditionally adopted regulators. In this work, we investigated...
Accurate predictive mathematical models are urgently needed in synthetic biology to support the bottom-up design of complex biological systems, minimizing trial-and-error approaches. The majority used so far adopt empirical Hill functions describe activation and repression exogenously-controlled inducible promoter systems. However, such equations may be poorly practical situations that typical design, including changes copy number, regulatory protein level, cell load. In this work, we...
Abstract The rational design of complex biological systems through the interconnection single functional building blocks is hampered by many unpredictability sources; this mainly due to tangled context-dependency behavior those parts once placed into an intrinsically living system. Among others, finite amount translational resources in prokaryotic cells leads load effects heterologous protein expression. As a result, hidden interactions among synthesis rates arise, leading unexpected and...
Engineering sustainable bioprocesses that convert abundant waste into fuels is pivotal for efficient production of renewable energy. We previously engineered an Escherichia coli strain optimized bioethanol from lactose-rich wastewater like concentrated whey permeate (CWP), a dairy effluent obtained valorization processes. Although attractive fermentation performances were reached, significant improvements are required to eliminate recombinant plasmids, antibiotic resistances and inducible...
Introduction Plant-pathogen interaction is an inexhaustible source of information on how to sustainably control diseases that negatively affect agricultural production. Meloidogyne incognita a root-knot nematode (RKN), representing pest for many crops, including tomato ( Solanum lycopersicum ). RKNs are global threat agriculture, especially under climate change, and RNA technologies offer potential alternative chemical nematicides. While endogenous microRNAs have been identified in both S....