A5076220004- Veterinary medicine and infectious diseases
- Concrete and Cement Materials Research
- Escherichia coli research studies
- Chromatin Remodeling and Cancer
- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Amoebic Infections and Treatments
- Hepatitis Viruses Studies and Epidemiology
- Toxin Mechanisms and Immunotoxins
- Advanced ceramic materials synthesis
- Hepatitis B Virus Studies
- Clostridium difficile and Clostridium perfringens research
- Healthcare Systems and Challenges
- Tumors and Oncological Cases
- Animal Disease Management and Epidemiology
- Cancer-related molecular mechanisms research
- Animal Virus Infections Studies
- T-cell and Retrovirus Studies
- Viral gastroenteritis research and epidemiology
- Pharmacological Effects of Natural Compounds
- Salmonella and Campylobacter epidemiology
Institut des Maladies Génétiques Imagine
2024
Université Paris Cité
2024
Hôpital Necker-Enfants Malades
2024
Assistance Publique – Hôpitaux de Paris
2024
Eli Lilly (United Kingdom)
2016-2017
Universidad de León
2008-2013
Post-weaning diarrhoea (PWD), due to Escherichia coli, is an important cause of economic losses the pig industry primarily as a result mortality and worsened productive performance. In spite its relevance, recent data about prevalence virulence genes pathotypes among E. coli isolates recovered from cases PWD in Europe are scarce.This study investigates fimbrial toxin by PCR 280 farms with across Europe. A total 873 samples collected within first 48 h after onset (occurring 7-21 days post...
The antimicrobial susceptibility of clinical isolates Brachyspira hyodysenteriae in Spain was monitored, and the underlying molecular mechanisms resistance were investigated. MICs tylosin, tiamulin, valnemulin, lincomycin, tylvalosin determined for 87 B. recovered from 2008 to 2009 by broth dilution. Domain V 23S rRNA gene ribosomal protein L3 sequenced 20 which tiamulin MIC ≥ 4 μg/ml, presenting decreased susceptibility, 18 tiamulin-susceptible (MIC ≤ 0.125 μg/ml), all typed multiple-locus...
F4- and F18-positive enterotoxigenic E. coli strains (F4-ETEC F18-ETEC) are important causes of post-weaning diarrhea (PWD) in pigs. F4 (antigenic variant ac) F18 (ab fimbriae major antigens that play an role the early stages infection. Herein, efficacy a live oral vaccine consisting two non-pathogenic strains, one F4ac- F18ac-positive, was evaluated using F4ac-ETEC F18ab-ETEC challenge models. A randomized, masked, placebo-controlled, block design, parallel-group confirmatory study with...
Enterotoxigenic Escherichia coli strains expressing F4 (K88) fimbriae (F4-ETEC) are one of the most important causes post-weaning diarrhea (PWD) in pigs. F4, a major antigen, plays an role early steps infection. Herein, efficacy live oral vaccine consisting non-pathogenic E. strain for protection pigs against PWD was evaluated. Three blinded, placebo-controlled, block design, parallel-group confirmatory experiments were conducted, using F4-ETEC challenge model, each with different...
Anseriformes deserve special attention in the epidemiology of Brachyspira spp. because diverse species have been described to act as highly efficient carriers several that can also infect livestock. The aim this study was investigate prevalence and diversity waterfowl winter Spain. were isolated from 51 205 faecal samples collected graylag geese mallards Villafáfila Lagoons Nature Reserve (Northwestern Spain). identified through phenotyping, PCR sequencing nox gene B. pilosicoli (5.9%),...
A total of sixty surgically castrated male pigs (Large White × Landrace) weighing 31·2 ( sd 4·3) kg were used in a randomised block experiment to examine the effect added dietary inulin (0, 20, 40 and 80 g/kg) on occurrence swine dysentery (SD) fermentation characteristics large intestine after experimental challenge with causative spirochaete Brachyspira hyodysenteriae . The allowed adapt diets for 2 weeks before each pig was challenged orally four times broth culture containing B....
ABSTRACT The spirochete Brachyspira hyodysenteriae is the causative agent of swine dysentery, a severe colonic infection pigs that has considerable economic impact in many swine-producing countries. In spite its importance, knowledge about global epidemiology and population structure B. limited. Progress this area been hampered by lack low-cost, portable, discriminatory method for strain typing. aim current study was to develop test multiple-locus variable-number tandem-repeat analysis...
SUMMARY This research aimed to describe the genetic and phenotypic diversity of 74 Spanish Brachyspira hyodysenteriae field isolates, establish epidemiological relationships between isolates confirm presence tiamulin-resistant in Spain. For these purposes, we performed biochemical tests combination with diagnostic PCR analysis for identification spp. detection smpA/smpB gene. We also used antimicrobial susceptibility tests, random amplified polymorphic DNA (RAPD) a new pulsed-field gel...
ABSTRACT Diagnostic wandering and delayed management are major issues in rare diseases. Here, we report a new Next‐Generation Phenotyping (NGP) model for diagnosing Coffin Siris syndrome (CSS) on clinical photographs among controls distinguish the different genotypes. This retrospective prospective study, conducted from 1998 to 2023, included frontal lateral pictures of confirmed CSS. After automatic placement landmarks, geometric features extraction using procrustes superimposition,...