A5031000732- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- Amino Acid Enzymes and Metabolism
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- Advanced Proteomics Techniques and Applications
- Microbial Community Ecology and Physiology
- Oral microbiology and periodontitis research
- Biochemical and Molecular Research
- Polyamine Metabolism and Applications
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Studies on Chitinases and Chitosanases
- Click Chemistry and Applications
- Plant nutrient uptake and metabolism
- Diet, Metabolism, and Disease
- Legume Nitrogen Fixing Symbiosis
- Water Treatment and Disinfection
- Methane Hydrates and Related Phenomena
- Metabolism and Genetic Disorders
University of Duisburg-Essen
2015-2025
Kiel University
2004-2008
Extracellular polymeric substances (EPS) are the major structural and functional components of microbial biofilms. The aim this study was to establish a method for EPS isolation from biofilms thermoacidophilic archaeon, Sulfolobus acidocaldarius, as basis analysis. Biofilms S. acidocaldarius were cultivated on surface gellan gum-solidified Brock medium at 78°C 4 days. Five extraction methods compared, including shaking biofilm suspensions in phosphate buffer, cation-exchange resin (CER)...
The oxidative Weimberg pathway for the five-step pentose degradation to α-ketoglutarate is a key route sustainable bioconversion of lignocellulosic biomass added-value products and biofuels. from Caulobacter crescentus has been employed in in-vivo metabolic engineering with intact cells in-vitro enzyme cascades. performance such approaches often hampered by systems complexity, caused non-linear kinetics allosteric regulatory mechanisms. Here we report an iterative approach construct validate...
Abstract Fatty acid metabolism and its regulation are known to play important roles in bacteria eukaryotes. By contrast, although certain archaea appear metabolize fatty acids, the of underlying pathways these organisms remains unclear. Here, we show that a TetR-family transcriptional regulator (FadR Sa ) is involved crenarchaeon Sulfolobus acidocaldarius . Functional structural analyses FadR binds DNA at semi-palindromic recognition sites two distinct stoichiometric binding modes depending...
Abstract Archaea are characterized by a unique life style in often environmental extremes but their thorough investigation is currently hampered limited set of suitable vivo research methodologies. Here, we demonstrate that activity-based protein profiling (ABPP) may be used to sensitively detect either native or heterogeneously expressed active enzymes living archaea even under these extreme conditions. In combination with the development genetically engineered archaeal screening strain,...
Summary Archaea are characterised by a complex metabolism with many unique enzymes that differ from their bacterial and eukaryotic counterparts. The thermoacidophilic archaeon Sulfolobus solfataricus is known for its metabolic versatility able to utilize great variety of different carbon sources. However, the underlying degradation pathways regulation often unknown. In this work, growth on sources was analysed, using an integrated systems biology approach. comparison L‐fucose D‐glucose...
Thermoacidophilic archaea belonging to the order Sulfolobales thrive in extreme biotopes, such as sulfuric hot springs and ore deposits. These microorganisms have been model systems for understanding life environments, well probing evolution of both molecular genetic processes central metabolic pathways. Thermoacidophiles, Sulfolobales, use typical microbial responses persist acid (e.g. motility, stress response, biofilm formation), albeit with some unusual twists. They also exhibit unique...
Cyanobacteria switch their carbon metabolism between photoautotrophy and heterotrophy during diurnal cycles. In cyanobacteria, the classical glycolytic control point is characterized by two catabolic phosphofructokinases (PFKs) a bifunctional anabolic fructose-1,6-biphosphatase/sedoheptulose-1,7-biphosphatase (F/SBPase; slr2094) catalyzing key reactions in Calvin-Benson-Bassham (CBB) cycle. addition, Synechocystis possesses fructose-1,6-bisphosphatase (FBPase; slr0952) with yet unknown...
Sulfolobus spp. possess a great metabolic versatility and grow heterotrophically on various carbon sources, such as different sugars peptides. Known sugar transporters in Archaea predominantly belong to ABC transport systems. Although several for uptake have been characterized the crenarchaeon solfataricus, only one homologue of these transporters, maltose/maltooligomer transporter, could be identified closely related acidocaldarius Comparison transcriptome S. MW001 grown peptides alone...
Enzymes from (hyper)thermophiles "Thermozymes" offer a great potential for biotechnological applications. Thermophilic adaptation does not only provide stability toward high temperature but is also often accompanied by higher resistance to other harsh physicochemical conditions, which are frequently employed in industrial processes, such as the presence of, e.g., denaturing agents well low or pH of medium. In order find new thermostable, xylan degrading hydrolases with application we used an...
Abstract Background Microbial communities are important drivers of global biogeochemical cycles, xenobiotic detoxification, as well organic matter decomposition. Their major metabolic role in ecosystem functioning is ensured by a unique set enzymes, providing tremendous yet mostly hidden enzymatic potential. Exploring this repertoire therefore not only relevant for better understanding how microorganisms function their natural environment, and thus ecological research, but further turns...
Abstract Glycerol is highly abundant in natural ecosystems and serves as both an important carbon source for microorganisms well a promising feedstock industrial applications. However, the pathways involved glycerol degradation Archaea remain unclear. Here, we show that thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius can grow with its sole characterize mechanisms utilization. We after uptake involving facilitated diffusion, phosphorylated to glycerol-3-phosphate by kinase (GK),...
In Archaea, acetate formation and ATP synthesis from acetyl-CoA is catalyzed by an unusual ADP-forming synthetase (ACD) (acetyl-CoA + ADP P(i) HS-CoA) catalyzing the of concomitant mechanism substrate level phosphorylation. ACD belongs to protein superfamily nucleoside diphosphate-forming acyl-CoA synthetases, which also include succinyl-CoA synthetases (SCSs). differs SCS in domain organization subunits presence a second highly conserved histidine residue beta-subunit, absent SCS. The...
The crenarchaeon Sulfolobus acidocaldarius has been described to synthesize trehalose via the maltooligosyltrehalose synthase (TreY) and trehalohydrolase (TreZ) pathway, glycosyltransferring (TreT) pathway predicted. Deletion mutant analysis of strains with single double deletions ΔtreY ΔtreT in S. revealed that addition these two pathways, a third, novel biosynthesis is operative vivo: trehalose-6-phosphate (T6P) synthase/T6P phosphatase (TPS/TPP) pathway. In contrast known TPS proteins,...
Activity-based protein profiling (ABPP) has so far scarcely been applied in Archaea general and, especially, extremophilic organisms. We herein isolated a novel Thermococcus strain designated sp. 2319x1E derived from the same enrichment culture as recently reported 2319x1. Both strains are able to grow with xylan sole carbon and energy source, for (optimal growth at 85°C, pH 6-7), induction of xylanolytic activity presence was demonstrated. Since solely sequence-based identification enzymes...
Abstract Sulfolobus acidocaldarius , a thermoacidophilic archaeon of the phylum Thermoproteota (former Crenarchaeota), is widely used model organism for gene deletion studies and recombinant protein production. Previous research has demonstrated efficacy saci_2122 promoter (P ara ), providing low basal activity high pentose-dependent induction. However, available expression vectors lack 5’-terminal untranslated region (5’-UTR), which typical element in bacterial vectors, usually...
Abstract Glycerol is highly abundant in nature and serve as carbon source for many organisms. Also, several Archaea have the genetic capacity to grow on glycerol but its degradation has so far only been studied Haloferax volcanii. Herein, thermoacidophilic crenarchaeon Sulfolobus acidocaldarius was shown with sole energy source. After uptake likely involving facilitated diffusion, degraded via phosphorylation glycerol-3-phosphate followed by oxidation dihydroxyacetone phosphate (DHAP)...
Abstract Archaea synthesize membranes using isoprenoid-based ether lipids, whereas Bacteria and Eukarya use fatty acid-based ester lipids. While the factors responsible for this “lipid divide” remain unclear, has important implications understanding evolutionary history of eukaryotes, which likely originated from within therefore changed membrane composition to Here, 13 C labelling studies, we demonstrate that archaeal model organisms Sulfolobus acidocaldarius Haloferax volcanii are capable...
Abstract Inorganic polyphosphate, a linear polymer of orthophosphate residues linked by phosphoanhydride bonds, occurs in all three domains life and plays diverse prominent role metabolism cellular regulation. While the polyphosphate its physiological significance have been well studied bacteria eukaryotes including human, there are only few studies archaea available so far. In Crenarchaeota members Sulfolobaceae , presence degradation via exopolyphosphatase has reported is some evidence for...
AMP‐forming acetyl‐CoA synthetases (ACSs) are ubiquitous in all three domains of life. Here, we report the first characterization an ACS from a hyperthermophilic organism, archaeon Pyrobaculum aerophilum . The recombinant ACS, gene product ORF PAE2867, showed extremely high thermostability and thermoactivity at temperatures around 100 °C. In contrast to known monomeric or homodimeric mesophilic ACSs, P. was 610 kDa homooctameric protein, with significant lower content thermolabile (Cys, Asn,...