A5081539043- Olfactory and Sensory Function Studies
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Acute Ischemic Stroke Management
- Physics of Superconductivity and Magnetism
- Advanced Chemical Sensor Technologies
- Terahertz technology and applications
- Cell Image Analysis Techniques
- Selenium in Biological Systems
- Receptor Mechanisms and Signaling
- Multiple Sclerosis Research Studies
- Photochromic and Fluorescence Chemistry
- Trace Elements in Health
- Gyrotron and Vacuum Electronics Research
- Superconducting and THz Device Technology
- Peripheral Neuropathies and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Neuroscience and Neural Engineering
- Nerve injury and regeneration
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- CRISPR and Genetic Engineering
- Spectroscopy and Laser Applications
Kyushu University
1995-2025
St. Marianna University School of Medicine
2013-2024
Kyoto University
2010-2023
University of Tsukuba
2004-2023
Japan Science and Technology Agency
2006-2023
The University of Tokyo
2003-2022
Tokyo Health Care University
2022
Kawasaki Municipal Hospital
2019-2021
Tokai University
2014-2019
Yokohama City Seibu Hospital
2016-2018
Abstract Development of a highly reproducible and sensitive single-cell RNA sequencing (RNA-seq) method would facilitate the understanding biological roles underlying mechanisms non-genetic cellular heterogeneity. In this study, we report novel RNA-seq called Quartz-Seq that has simpler protocol higher reproducibility sensitivity than existing methods. We show can quantitatively detect various kinds heterogeneity, different cell types cell-cycle phases single type. Moreover, comprehensively...
In mammals, odorant receptors (ORs) direct the axons of olfactory sensory neurons (OSNs) toward targets in bulb. We show that cyclic adenosine monophosphate (cAMP) signals regulate expression axon guidance molecules are essential for OR-instructed axonal projection. Genetic manipulations ORs, stimulatory G protein, cAMP-dependent protein kinase, and cAMP response element–binding shifted projection sites along anteriorposterior axis Thus, it is OR-derived signals, rather than action OR...
Highlights•SeeDB2 is a simple optical clearing method preserving morphology and fluorophores•SeeDB2 optimized for high-resolution imaging by minimizing spherical aberrations•Volumetric super-resolution enables synaptic-scale connectomic studies•Quantitative synapse mapping demonstrated mouse fly brain samplesSummarySuper-resolution deep inside tissues has been challenging, as it extremely sensitive to light scattering aberrations. Here, we report an agent fluorescence (SeeDB2). SeeDB2...
Sensory information detected by the peripheral nervous system is represented as a topographic map in brain. It has long been thought that topography of determined graded positional cues are expressed target. Here, we analyzed pre-target axon sorting for olfactory formation mice. In sensory neurons, an guidance receptor, Neuropilin-1, and its repulsive ligand, Semaphorin-3A, complementary manner. We found expression levels Neuropilin-1 both projection sites axons. Olfactory neuron-specific...
In developing brains, activity-dependent remodeling facilitates the formation of precise neuronal connectivity. Synaptic competition is known to facilitate synapse elimination; however, it has remained unknown how different synapses compete with one another within a post-synaptic cell. Here, we investigate mitral cell in mouse olfactory bulb prunes all but primary dendrite during developmental process. We find that spontaneous activity generated essential. show strong glutamatergic inputs...
Sensory information is selectively or non-selectively enhanced and inhibited in the brain, but it remains unclear whether how this occurs at most peripheral level. Using vivo calcium imaging of mouse olfactory bulb epithelium wild-type mutant animals, we show that odors produce not only excitatory also inhibitory responses sensory neurons (OSNs). Heterologous assays indicate odorants can act as agonists to some inverse other odorant receptors. We demonstrate odor mixtures are extensively...
The stochastic multicolor labeling method ‘Brainbow’ is a powerful strategy to label multiple neurons differentially with fluorescent proteins; however, the fluorescence levels provided by original attempts use this were inadequate. In present study, we developed enhanced expression that uses tetracycline-operator system (Tetbow). We optimized Tetbow for either plasmid or virus vector-mediated labeling. When combined tissue clearing, was enough visualize three-dimensional architecture of...
Abstract Vertebrate odorant receptor (OR) genes are divided phylogenetically into two distinct classes: the fish‐like class I and terrestrial‐specific II. In present study, we systematically analysed mouse OR (42 subfamilies) to elucidate expression profiles in olfactory epithelium (OE) projection sites of their sensory neurons (OSNs) bulb (OB). situ hybridization (ISH) revealed that most (36 were expressed dorso‐medial zone (zone 1) OE. Furthermore, there appeared be no significant...
Abstract Large‐scale three‐dimensional fluorescence imaging is essential for comprehensive and quantitative understanding of neuronal circuitry. We describe a water‐based optical clearing agent, SeeDB, which clears fixed brain samples in few days leaving many types fluorescent dyes unquenched, including proteins lipophilic tracers. This method maintains constant sample volume during the procedure, an important factor to keep cellular morphology intact. After with we can reach depth >1000...
For high luminosity in electron-positron linear colliders, it is essential to generate low vertical emittance beams. We report on the smallest achieved single-bunch-mode operation of Accelerator Test Facility, which satisfies requirement x-band collider. The emittances were measured with a laser-wire beam-profile monitor installed damping ring. bunch length and momentum spread beam also recorded under same conditions. rms at intensity 4 pm energy 1.3 GeV, corresponds normalized 1.0x1.0(-8)...
Abstract Investigations with the electron microscope on γ-irradiated single crystals of LiH have revealed presence cubic voids, or bubbles. These bubbles are crystallographically oriented, principally their sides parallel to (100) planes crystals. Bubbles range in size from ∼ 75 Å 1500 Å. Average bubble increases increasing irradiation dose and temperature, temperature affecting most, by far. believed be responsible for large swelling seen irradiated Souers, Blake, Penpraze Cline (1969).
Abstract In rodents, olfactory receptor (OR) genes are expressed in one of four zones the epithelium (OE), and sensory neurons (OSNs) expressing same OR project their axons to a specific set glomeruli on bulb (OB). Using yeast artificial chromosome (YAC) transgenic system, we have analysed expression murine gene MOR29A MOR28 cluster located 14. Although endogenous was restricted most dorsomedial zone, (Tg ) all OE. When OB mouse analysed, OSNs Tg were found not only dorsal side but also...
Oxidative stress, caused by the over production of reactive oxygen species (ROS), has been shown to contribute cell damage associated with neurotrauma and neurodegenerative diseases. ROS mediates either through direct oxidation lipids, proteins DNA or acting as signaling molecules trigger cellular apoptotic pathways. The 78 kDa glucose-regulated protein (GRP78) is an ER chaperone that suggested protect cells against ROS-induced damage. However, protective mechanism GRP78 remains unclear. In...