A5055074021- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Advanced Fluorescence Microscopy Techniques
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Metal-Catalyzed Oxygenation Mechanisms
- Cancer, Hypoxia, and Metabolism
- Signaling Pathways in Disease
- Histone Deacetylase Inhibitors Research
University of Cambridge
2012-2023
The nucleosome remodeling deacetylase (NuRD) complex is a highly conserved regulator of chromatin structure and transcription. Structural studies have shed light on this other modifying machines, but much less known about how they assemble whether stable functional sub-modules exist that retain enzymatic activity. Purification the endogenous Drosophila NuRD shows it consists core subunits, while others, in particular remodeler CHD4, associate transiently. To dissect assembly activity NuRD,...
Post-translational modification of histone proteins are known to play an important role in regulating chromatin structure. In effort find additional modifications we set out screen enzymes the 2-oxoglutarate and Fe(II)-dependent (2-OG-Fe(II)) dioxygenase family for activity towards histones. Here show that Schizosaccharomyces pombe 2-OG-Fe(II) domain containing protein-2 (Ofd2) is a H2A enzyme. Using combination peptide screening alanine scanning substitution analysis, identify HxxLR motif...