A5089492935- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Bacterial Infections and Vaccines
- Bacterial biofilms and quorum sensing
- Infective Endocarditis Diagnosis and Management
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Bacteriophages and microbial interactions
- Veterinary medicine and infectious diseases
- Advanced Biosensing Techniques and Applications
- Protein purification and stability
- Reproductive tract infections research
- Concrete and Cement Materials Research
- Microbial infections and disease research
- Pharmacological Effects of Natural Compounds
- Aortic Thrombus and Embolism
- Graphene and Nanomaterials Applications
- T-cell and B-cell Immunology
- Fungal Infections and Studies
- Complement system in diseases
- Biochemical and Structural Characterization
- Orthopedic Infections and Treatments
- Otolaryngology and Infectious Diseases
- Urinary Tract Infections Management
- Advanced ceramic materials synthesis
University Medical Center Utrecht
2001-2022
Heidelberg University
2022
University Hospital Heidelberg
2022
Utrecht University
1995-2021
Eijkman Institute for Molecular Biology
1998
Peripheral blood leukocytes incubated with a semisynthetic phage antibody library and fluorochrome-labeled CD3 CD20 antibodies were used to isolate human single-chain Fv specific for subsets of by flow cytometry. Isolated showed exclusive binding the subpopulation selection or displayed additional restricted population other cells in mixture. At least two appeared display hitherto-unknown staining patterns B-lineage cells. This approach provides subtractive procedure rapidly obtain against...
Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a often insensitive antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach improve the diagnosis potential treatment biofilm-related infections. Here, we show that mAbs targeting common surface components S. can recognize clinically relevant types. The were also shown bind collection clinical isolates derived from different...
Abstract Both IgG and IgA Abs have been implicated in host defense against bacterial infections, although their relative contributions remain unclear. We generated a unique panel of human chimeric all subclasses with identical V genes porin A, major subcapsular protein Ag Neisseria meningitidis vaccine candidate. Chimeric were produced baby hamster kidney cells, IgA-producing clones cotransfected J chain and/or secretory component. Although (isotypes IgG1–3) mediated efficient...
Ion-assisted plasma polymerization for single-step, covalent immobilization of silver nanoparticles on surfaces.
Brachyspira species are fastidious anaerobic microorganisms, that infect the colon of various animals. The genus contains both important pathogens livestock as well commensals. Two known to humans: B. aalborgi and pilosicoli. There is some evidence suggesting veterinary pathogenic pilosicoli a potential zoonotic agent, however, since diagnosis in humans based on histopathology biopsies, identification not routinely performed human materials.The study population comprised 57 patients with...
Urinary Tract Infections (UTIs) are among the most frequently occurring infections in hospital. Urinalysis and urine culture main tools used for diagnosis. Whereas urinalysis is sufficiently sensitive detecting UTI, it has a relatively low specificity, leading to unnecessary treatment with antibiotics risk of increasing antibiotic resistance. We performed an evaluation current diagnostic process expert-based label UTI as outcome, retrospectively established using data from Electronic Health...
Class 1 outer membrane protein PorA of Neisseria meningitidis is a vaccine candidate against bacterial meningitis. Antibodies are able to induce complement-mediated killing and thereby play an important role in protection meningococcal disease. Bactericidal antibodies all directed variable regions VR1 VR2 the sequence, corresponding loops 4 two-dimensional topology model porin with eight extracellular loops. We have determined crystal structure 2.6 Å resolution Fab fragment bactericidal...
ABSTRACT We used competitive panning to select a panel of 10 different human antibodies from large semisynthetic phage display library that distinguish between serum complement-resistant and complement-sensitive strains the gram-negative diplococcus Moraxella ( Branhamella ) catarrhalis . Western blotting analyses inhibition enzyme-linked immunosorbent assays showed all were directed against same or closely spaced epitopes on target protein, which is high-molecular-weight outer membrane...
Class 1 outer membrane protein PorA of Neisseria meningitidis is a vaccine candidate against bacterial meningitis. Antibodies are able to induce complement-mediated killing and thereby play an important role in protection meningococcal disease. Bactericidal antibodies all directed variable regions VR1 VR2 the sequence, corresponding loops 4 two-dimensional topology model porin with eight extracellular loops. We have determined crystal structure 2.6 Å resolution Fab fragment bactericidal...
Abstract Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a often insensitive antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach improve the diagnosis and/or treatment biofilm-related infections. Here we show that mAbs targeting common surface components S. can recognize clinically relevant types. We identify two groups antibodies: one group uniquely binds state recognizes both...
Abstract Background Infective endocarditis (IE) was associated with poor survival. In 2015, the European Society of Cardiology (ESC) introduced multidisciplinary IE team to improve diagnostics, treatment and outcome in patients IE. Purpose This study evaluated clinical implementation Netherlands. Methods Retrospective cohort all diagnosed between 2012–2016 a tertiary single center. Clinical characteristics, data on imaging techniques, surgical procedures, IE-related complications all-cause...