Zinc is essential for the growth of human and other animals. Bone retardation a common finding in various conditions associated with zinc deficiency, suggesting physiological role mineralization bone tissue. content decreased by development aging, skeletal unloading, postmenopausal conditions. deficiency may play pathophysiological deterioration metabolism. has been demonstrated to have stimulatory effect on formation mineralization; metal directly activates aminoacyl-tRNA synthetase...

A calcium binding substance has been isolated from the soluble fraction of normal rat liver. The was purified by means gel filtration on Sephadex G-75 and G-50 followed ion-exchange chromatography diethylaminoethyl-cellulose. By these methods, activity several 100-fold homogeneity established polyacrylamide electrophoresis.

Regucalcin was discovered in 1978 as a Ca(2+)-binding protein that does not contain EF-hand motif of domain. The name regucalcin proposed for this Ca2(2+)binding protein, which can regulate liver cell functions related to Ca(2+). gene is localized on chromosome X, and the organization consists seven exons six introns. AP-1 NFI-A1 bind promoter region rat mediate Ca(2+) response transcriptional activation. plays pivotal role maintaining intracellular homeostasis due activating pump enzymes...

Osteoporosis and bone fractures are increasingly recognized complications of HIV-1 infection. Although antiretroviral therapy itself has complex effects on turnover, it is now evident that the majority HIV-infected individuals already exhibit reduced mineral density before therapy. The mechanisms responsible likely multifactorial have been difficult to delineate in humans. transgenic rat recapitulates many key features human AIDS. We demonstrate that, like their counterparts, rats undergo...

The distribution and expression of mRNA encoding the Ca(2+)-binding protein regucalcin in rats were investigated by Northern blot analyses. Liver cDNA (0.6 kb) was used as a probe. analyses total RNAs extracted from various tissues rat indicated that mainly present liver but only slightly kidney with size 1.8 kb. level decreased increasing age (3, 10 25 weeks). A single intraperitoneal administration calcium chloride (15 mg Ca/100 g body weight) induced remarkable increase liver; about 200%...

Several bone protective factors are reported to exhibit stimulatory activities on formation coupled with inhibitory effects resorption; one such factor is vitamin K2. Vitamin K species [K1 (phylloquinone) and K2 (menaquinone)] have long been associated receiving intense interest as nutritional supplements for the prevention or amelioration of disease in humans. However, mechanisms action skeleton poorly defined. Activation nuclear κB (NF-κB) signal transduction pathway essential osteoclast...

HIV infection causes bone loss. We previously reported that immunosuppression-mediated B-cell production of receptor activator NF-κB ligand (RANKL) coupled with decline in osteoprotegerin correlate decreased mineral density (BMD) untreated infection. Paradoxically, antiretroviral therapy (ART) worsens loss although existing data suggest such is largely independent specific regimen. This led us to hypothesize skeletal deterioration following disease reversal ART may be related T-cell...

Genistein is a natural isoflavone found in Leguminosae. The effect of genistein on osteoblastic MC3T3-E1 cells was investigated. Cells were cultured for 48 h the presence (10(-7)-10(-5) M). (10(-6) and 10(-5) M) caused significant elevation protein content, alkaline phosphatase activity, deoxyriboncleic acid (DNA) content cells. (10-5 increasing activity DNA completely prevented by cycloheximide M), an inhibitor synthesis, suggesting that isoflavone's results from newly synthesized...

Bone loss with increasing age induces osteoporosis. This may be due to increased bone resorption and decreased formation. Osteoporosis a decrease in mass is widely recognized as major public health problem. Pharmacological nutritional factors prevent age. The chemical compounds food that act on metabolism, however, are poorly understood. Genistein natural isoflavonoid phytoestrogen found Leguminosae has been demonstrated have an anabolic effect suggesting its role the prevention of...

Royal jelly (RJ) has been used worldwide for many years as medical products, health foods and cosmetics. Since RJ contains testosterone steroid hormone‐type activities, we hypothesized that it may have beneficial effects on osteoporosis. We both an ovariectomized rat model a tissue culture model. Rats were divided into eight groups follows: sham‐operated (Sham), (OVX), OVX given 0.5% (w/w) raw RJ, 2.0% protease‐treated (pRJ), pRJ, 17β‐estradiol its vehicle, respectively. The Ovariectomy...