A5036792892- Biocrusts and Microbial Ecology
- Tardigrade Biology and Ecology
- Spaceflight effects on biology
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Effects of Radiation Exposure
- Heat shock proteins research
- Cancer, Hypoxia, and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- Photodynamic Therapy Research Studies
- Redox biology and oxidative stress
- Astro and Planetary Science
- Biofield Effects and Biophysics
- Electromagnetic Fields and Biological Effects
- Insect Resistance and Genetics
- Hemoglobin structure and function
- Insect symbiosis and bacterial influences
- Nuclear Issues and Defense
- Insect and Pesticide Research
Joint Institute for Nuclear Research
2020-2024
The tardigrade-unique damage suppressor protein (Dsup) can protect DNA from ionizing radiation and reactive oxygen species (ROS). In this study, we generated Dsup-expressing lines of Drosophila melanogaster demonstrated that Dsup increased the survival rate after γ-ray irradiation hydrogen peroxide treatment in flies too, but reduced level their locomotor activity. transcriptome analyses revealed a significant number DEGs, >99% which were down-regulated. Moreover, could bind RNA. These...
Endogenous autofluorescence of biological tissues is an important source information for biomedical diagnostics. Despite the molecular complexity tissues, list commonly known fluorophores strictly limited. Still, question sources red and near-infrared excited remains open. In this work we demonstrated that oxidation products organic components (lipids, proteins, amino acids, etc.) can serve as such autofluorescence. Using model solutions cell systems (human keratinocytes) under oxidative...
Natural background radiation is a permanent multicomponent factor. It has an influence on biological organisms, but effects of its deprivation still remain unclear. The aim our work was to study for the first time responses D . melanogaster conditions Deep Underground Low-Background Laboratory DULB-4900 (BNO, INR, RAS, Russia) at transcriptome level by RNA-seq profiling. Overall 77 transcripts demonstrated differential abundance between flies exposed low and natural radiation. Enriched...
Abstract When developing functionalized biomaterials, the proteins from extremophilic organisms, in particular unique tardigrade disordered proteins, are of great value. The damage suppressor protein (Dsup), initially discovered Ramazzottius varieornatus and found to be an efficient DNA protector under oxidative irradiation stress, has been hypothesized possess a good potential for development material, which can isolate cell‐free DNA. With this mind, DNA‐nonadsorbing polyethylene...
Positive selection may be the main factor of between-population divergence in gene expression. Expression profiles two Drosophila melanogaster laboratory strains different geographical origin and long-term maintenance were analyzed using microchip arrays encompassing probes for 18,500 transcripts. The Russian strain D18 North American Canton-S compared. A set 223 known or putative genes demonstrated significant changes expression levels between these strains. Differentially expressed (DEG)...
The unique Dsup protein of the tardigrade Ramazzottiusvarieornatus increases resistance to radiation and oxidative stress in various species organisms human cell culture. According modeling data,Dsup forms a complex with DNA an intermolecular distance ∼4˚A, as result which is lessdamaged by reactive oxygen formed during exposure radiation.However, stability Dsupprotein itself under uence ionizing remains unclear, important for assessing its radioprotective potential understanding molecular...
We report 19 metagenome-assembled genomes from a deep underground microbial community found in mineralized hydrothermal spring the Baksan Neutrino Observatory tunnel. The is predominantly occupied by members of Pseudomonadota (Gamma-, Beta-, and Alphaproteobacteria), Planctomycetota, Myxococcota, Nitrospirota, Cyanobacteria, Gemmatimonadota, Armatimonadota.