A5015219111- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Advanced Fluorescence Microscopy Techniques
- Collagen: Extraction and Characterization
- Pluripotent Stem Cells Research
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Monoclonal and Polyclonal Antibodies Research
- Molecular Junctions and Nanostructures
- Force Microscopy Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Spectroscopy and Quantum Chemical Studies
- Microfluidic and Bio-sensing Technologies
- Gene Regulatory Network Analysis
- Image Processing Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanoparticle-Based Drug Delivery
- Digital Imaging for Blood Diseases
- Analytical Chemistry and Sensors
- Genetics, Bioinformatics, and Biomedical Research
- Biomedical Ethics and Regulation
- Single-cell and spatial transcriptomics
National Institute of Standards and Technology
2015-2025
Material Measurement Laboratory
2011-2024
National Institute of Standards
1990-2022
Government of the United States of America
2022
Indiana University
2021
University of Miami
2021
Children's Cancer Center
2021
U.S. National Science Foundation
2021
Cancer Institute (WIA)
2021
United States Department of Commerce
2020
Supported planar lipid bilayers based on alkanethiol-tethering chemistry are becoming increasingly important biomimetic materials. Hybrid containing thiol-derivatized alkane moieties plus natural lipids provide a matrix that permits the successful reconstitution of membrane protein activity. The hybrid is self-assembled and sufficiently rugged to be practical use in research commercial sensing applications. coupling bilayer metal support allows wide range analytical techniques applied this...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelf-assembled phospholipid/alkanethiol biomimetic bilayers on goldAnne L. PlantCite this: Langmuir 1993, 9, 11, 2764–2767Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November 1993https://doi.org/10.1021/la00035a004RIGHTS & PERMISSIONSArticle Views1738Altmetric-Citations288LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since 2008 (both...
Supported hybrid bilayer membranes (HBM) composed of a monolayer phospholipid and alkanethiol associated with thin gold film on glass are useful as model lipid for studying membrane receptor-ligand cell-cell binding events by surface plasmon resonance (SPR). Measurements specific proteins vesicles to well-defined HBMs have been performed under conditions continuous flow using commercial SPR instrument (BIAcore). shown be stable in block nonspecific adsorption the alkanethiol/gold surface....
Photobleaching and related photochemical processes are recognized experimental barriers to quantification of fluorescence by microscopy. We have measured the kinetics photobleaching fluorophores in living fixed cells microemulsions, demonstrated spatial variability these within individual cells. An inverted microscope a high-sensitivity camera, together with high-speed data acquisition computer-controlled image processor, been used control precisely exposure time excitation light record...
Different genomic technologies have been applied to cell line authentication, but only one method (short tandem repeat [STR] profiling) has the subject of a comprehensive and definitive standard (ASN-0002). Here we discuss power this document why standards such as are so critical for establishing consensus technical criteria practices that can enable progress in fields research use lines. We also examine other methods could be used authentication how combination holistic fashion assess...
Surface plasmon resonance imaging (SPRI) is a label-free technique that can image refractive index changes at an interface. We have previously used SPRI to study the dynamics of cell-substratum interactions. However, characterization spatial resolution in 3 dimensions necessary quantitatively interpret SPR images. Spatial complicated by asymmetric propagation length surface plasmons x and y leading degradation one direction. Inferring distance intracellular organelles other subcellular...
The analysis of fluorescence microscopy cells often requires the determination cell edges. This is typically done using segmentation techniques that separate objects in an image from surrounding background. study compares results nine different applied to two lines and five sets imaging conditions. Significant variability was observed due solely differences conditions or applications algorithms. We quantified compared with a novel bivariate similarity index metric evaluates degree...
The interaction of small phospholipid vesicles with well-characterized surfaces has been studied to assess the effect surface free energy underlying monolayer on formation phospholipid/alkanethiol hybrid bilayer membranes (HBMs). was changed in a systematic manner using single-component alkanethiol monolayers and binary mixtures thiols. were prepared gold by self-assembly from solutions thiols HS-(CH(2))(n)()-X (n = 11, X CH(3) or OH) THF. Surface plasmon resonance (SPR), electrical...
Hybrid bilayer membranes (HBMs), consisting of a lipid monolayer covering self-assembled alkanethiol on metal surface, are useful models for studying the structure and function cell membranes. Surface-enhanced Raman spectroscopy (SERS) reflection absorption infrared (RAIRS) used to study HBMs with various components. Together, these two techniques clearly indicate that forms well-ordered, non-interdigitated layer head groups lipids oriented away from surface. Both reveal formation an HBM...
Thin films of the extracellular matrix protein, collagen, were prepared by adsorbing native or heat-denatured type I collagen onto hexadecanethiol self-assembled monolayers. The resulting characterized atomic force microscopy, ellipsometry, and light microscopy. Denatured formed a topographically smooth ∼3.6 nm thick film, consistent with an adsorbed protein monolayer. In contrast, thin film consisted supramolecular fibrils. density large fibrils could be varied changing concentration in...
We have developed a liposome-based flow injection immunoassay (FIIA) system for quantitation of clinical analyte, theophylline. With very minor changes in assay format, this procedure can also be used the anti-theophylline. Automated sequential analyses were performed at room temperature with picomole sensitivity and day-to-day coefficient variation less than 5% aqueous solutions. The components include liposomes that contain fluorophores their centers an immobilized-antibody reactor column....
A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and active remodeling by ECM. Fluorescence microscopy a commonly employed technique for examining cell-matrix interactions. label-free imaging method would provide an alternative that eliminate requirement transfected modified biological molecules, if collected nondestructively, allow long term observation analysis live cells. Using surface plasmon resonance (SPRI),...
Oxygen tension in mammalian cell culture can profoundly affect cellular differentiation, viability, and proliferation. However, precise measurement of dissolved oxygen real time remains difficult. We report a new noninvasive sensor that accurately measure concentration during while being compatible with live-cell imaging techniques such as fluorescence phase contrast microscopy. The is prepared by integrating the porphyrin dye, Pt(II) meso-tetrakis(pentafluorophenyl)porphine (PtTFPP) into...
The surface plasmon resonance technique allows direct, real-time kinetic measurements of the interaction unlabelled biological molecules at surfaces. After a brief discussion principles resonance, we review its application to nonspecific adsorption protein, formation phospholipid bilayers, membrane-protein interactions and DNA hybridization. morphological studies surfaces using microscopy potential measure dynamics layer heterogeneity are discussed.
The mechanism for the formation of biomimetic model cell membranes consisting bilayers composed alkanethiols and phospholipids was probed with a kinetic study using surface plasmon resonance. kinetics monolayer phospholipid from vesicles in solution onto hydrophobic alkanethiol is described by that takes into account lipid concentration, diffusion, reorganization rate constant. Monomer apparently does not play direct role determining bilayer formation. Expressions limiting cases this...
Recent improvements in neutron reflectometry methodology have afforded enhanced sensitivity for the study of biomimetic membranes. The technique has been used to probe interactions peptide toxin, melittin, with supported bilayers phospholipid and octadecanethiol or thiahexa(ethylene oxide) alkane on gold. Improvements instrumentation experimental design permit reflectivity measurements out a wavevector transfer 0.7 Å-1 down reflectivities approaching 10-8, allowing unprecedented resolution...
Uptake of benzo(a)pyrene by living cultured cells has been visualized in real time using digital fluorescence-imaging microscopy. Benzo(a)pyrene was noncovalently associated with lipoproteins, as a physiologic mode presentation the carcinogen to cells. When incubated either human fibroblasts or murine P388D1 macrophages, uptake occurred absence endocytosis, halftime approximately 2 min, irrespective identity delivery vehicles, which were high density low very and...