A5016659008- Venous Thromboembolism Diagnosis and Management
- Blood Coagulation and Thrombosis Mechanisms
- Platelet Disorders and Treatments
- Adenosine and Purinergic Signaling
- Blood properties and coagulation
- Atrial Fibrillation Management and Outcomes
- Antiplatelet Therapy and Cardiovascular Diseases
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Acute Ischemic Stroke Management
- Cellular transport and secretion
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Mechanical Circulatory Support Devices
- Eosinophilic Disorders and Syndromes
- Ubiquitin and proteasome pathways
- Acute Myocardial Infarction Research
- Pulmonary Hypertension Research and Treatments
- Cardiac Ischemia and Reperfusion
- Glycosylation and Glycoproteins Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Renal Transplantation Outcomes and Treatments
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Neurological Complications and Syndromes
- Travel-related health issues
Monash University
2016-2025
Alfred Health
2017-2025
Northern Health
2017-2025
Australian Centre for HIV and Hepatitis Virology Research
2015-2024
Australian Regenerative Medicine Institute
2017-2024
Monash Health
2024
St Vincent's Hospital
2010-2023
The University of Melbourne
2006-2023
Monash Alfred Psychiatry Research centre
2023
The Alfred Hospital
2017
Extracellular nucleotides play an important role in thrombosis and inflammation, triggering a range of effects such as platelet activation recruitment, endothelial cell activation, vasoconstriction. CD39, the major vascular nucleoside triphosphate diphosphohydrolase (NTPDase), converts ATP ADP to AMP, which is further degraded antithrombotic anti-inflammatory mediator adenosine. Deletion CD39 renders mice exquisitely sensitive injury, CD39-null cardiac xenografts show reduced survival....
Abstract New oral anticoagulants ( NOAC ) are becoming available as alternatives to warfarin prevent systemic embolism in patients with non‐valvular atrial fibrillation and for the treatment prevention of venous thromboembolism. An in‐depth understanding their pharmacology is invaluable appropriate prescription optimal management receiving these drugs should unexpected complications (such bleeding) occur, or patient requires urgent surgery. The Australasian S ociety T hrombosis H aemostasis...
Introduction Venous thromboembolism (VTE), including deep vein thrombosis (DVT) and pulmonary embolism (PE), is the third most common cardiovascular disease and, globally, more than an estimated 10 million people have it yearly. It a chronic recurrent disease. The symptoms of VTE are non-specific diagnosis should actively be sought once considered. mainstay treatment anticoagulation, with few patients requiring additional intervention. A working group experts in area recently completed...
The Coronavirus disease 2019 (COVID)-19 pandemic has already affected millions worldwide, with a current mortality rate of 2.2%. While it is well-established that severe acute respiratory syndrome-coronavirus-2 causes upper and lower tract infections, number neurological sequelae have now been reported in large proportion cases. Additionally, the arterial venous thromboses including pulmonary embolism, myocardial infarction, significant cerebrovascular complications. increasing incidence...
SHIP-2 is a phosphoinositidylinositol 3,4,5 trisphosphate (PtdIns[3,4,5]P3) 5-phosphatase that contains an NH2-terminal SH2 domain, central and COOH-terminal proline-rich domain. negatively regulates insulin signaling. In unstimulated cells, localized in perinuclear cytosolic distribution at the leading edge of cell. Endogenous recombinant to membrane ruffles, which were mediated by proline–rich To identify proteins bind yeast two-hybrid screening was performed, isolated actin-binding...
The vascular ectonucleotidases CD39 [ENTPD1 (ec-tonucleoside triphosphate diphosphohydrolase-1), EC 3.6.1.5] and CD73 [EC 3.1.3.5] generate adenosine from extracellular nucleotides. activity is critical in de-termining the response to ischemia-reperfusion injury (IRI), null mice exhibit heightened sensitivity renal IRI. Adenosine has multiple mechanisms of action vasculature including direct endothelial protection, antiinflammatory antithrombotic effects protective several models Mice...
Rationale No evidence-based acute therapies exist for intracerebral hemorrhage. Intracerebral hemorrhage growth is an important determinant of patient outcome. Tranexamic acid known to reduce in other conditions. Aim The study aims test the hypothesis that patients selected with computed tomography angiography contrast extravasation ‘spot sign’ will have lower rates hematoma when treated intravenous tranexamic within 4·5-hours stroke onset compared placebo. Design Spot sign and On Preventing...
CD39 is a cell membrane NTPase with anti-inflammatory and anti-platelet effects. However, its clinical use limited by bleeding side effect. With the goal of harnessing therapeutic potential while avoiding haemostatic problems, we designed fusion protein consisting extracellular domain single-chain antibody (Targ-CD39) that specifically binds to activated glycoprotein (GP)IIb/IIIa thus platelets. Through this enrichment at platelets, required systemic dose below impairing haemostasis.Using an...
Objective— Hypothermia is used in various clinical settings to inhibit ischemia-related organ damage. However, prothrombotic effects have been described as potential side effects. This study aimed elucidate the mechanism of hypothermia-induced platelet activation and subsequent events develop preventative pharmacological strategies applicable during clinically hypothermia. Methods Results— Platelet function was investigated ex vivo at hypothermia (28°C/18°C). Hypothermic mice demonstrated...
Myotubularin is a dual-specific phosphatase that dephosphorylates phosphatidylinositol 3-phosphate and (3,5)-bisphosphate. Mutations in myotubularin result the human disease X-linked myotubular myopathy, characterized by persistence of muscle fibers retain an immature phenotype. We have previously reported identification 3-phosphatase adapter protein (3-PAP), catalytically inactive member gene family, which coprecipitates lipid 3-phosphate-3-phosphatase activity from lysates platelets. now...
Four and a half LIM domain (FHL) proteins are members of the protein superfamily. Several FHL function as co-activators CREM/CREB transcription factors androgen receptor. FHL3 is highly expressed in skeletal muscle, but its unknown. localized to nucleus C2C12 myoblasts and, following integrin engagement, exited actin stress fibers focal adhesions. In mature muscle was found at Z-line. Actin identified potential binding partner yeast two-hybrid screening library. complexed with both vitro...
An N-ethyl-N-nitrosourea mutagenesis screen in mice was performed to isolate regulators of circulating platelet number. We report here recessive thrombocytopenia and kidney disease plt1 mice, which is the result a severe but partial loss-of-function mutation gene encoding glycoprotein-N-acetylgalactosamine-3-beta-galactosyltransferase (C1GalT1), an enzyme essential for synthesis extended mucin-type O-glycans. Platelet half-life basic hemostatic parameters were unaffected plt1/plt1 not...
Platelet activation is believed to play an important role in the triggering of thrombosis human blood by pig islets. We used a transgenic mouse model investigate whether overexpression CD39 (ecto nucleoside triphosphate diphosphohydrolase 1 [ENTPD1], EC 3.6.1.5), ectonucleotidase that degrades platelet agonists ATP, could interfere with this process. Islets isolated from mice showed 2.4-fold higher NTPDase activity than wild-type controls. When incubated blood, these islets significantly...
The risk of infection with Pneumocystis jirovecii pneumonia (PCP) in patients undergoing chemotherapy is closely related to the intensity corticosteroid exposure. PCP uncommon classical (3-weekly) R-CHOP, but may be higher biweekly R-CHOP (R-CHOP-14) due increased frequency prednisolone pulses. Among 47 consecutive treated R-CHOP-14 at our institution, five (11%) developed microbiologically proven PCP, a further two (4%) having clinical and radiological features without microbiological...