A5086615062- Advanced Proteomics Techniques and Applications
- Plant Stress Responses and Tolerance
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Seed Germination and Physiology
- Allelopathy and phytotoxic interactions
- Plant biochemistry and biosynthesis
- Fungal and yeast genetics research
- Metabolomics and Mass Spectrometry Studies
- Plant nutrient uptake and metabolism
- Mycotoxins in Agriculture and Food
- Sunflower and Safflower Cultivation
- Plant and soil sciences
- Mass Spectrometry Techniques and Applications
- Plant pathogens and resistance mechanisms
- Nematode management and characterization studies
- Fungal Plant Pathogen Control
- Weed Control and Herbicide Applications
- Forest ecology and management
University of Córdoba
2016-2025
Cordoba University
2007-2016
Andalusian Institute of Agricultural and Fisheries Research and Training
2014
Hospital de Cruces
2011
Universidad de Oviedo
2008-2010
Instituto de Agricultura Sostenible
2005
Universidad de Cádiz
2002
Durham University
1997
Noble Research Institute
1990
Based on the presence of phospholipids in extracellular fluids (EFs) sunflower seeds, we have hypothesized existence vesicles apoplastic compartment plants. Ultracentrifugation EF allowed isolation particles 50‐200 nm with apparent membrane organization. A small GTPase Rab was putatively identified this vesicular fraction. Since proteins are involved traffic and their exosomes from animal has been demonstrated, evidence presented here supports exosome‐like sunflower. Their putative...
The interaction of the parasitic plant Orobanche cernua with resistant and susceptible cultivars Helianthus annuus L. was investigated. Using different bioassays to evaluate early stages parasite life cycle (germination, attachment, penetration, establishment), differences were observed between O. cernua‐resistant cernua‐susceptible sunflower varieties. Germination seeds in presence roots approximately half that germination roots, germinated displayed enhanced browning symptoms. Parasite...
The necrotrophic fungus Botrytis cinerea is a very damaging phytopathogen of wide host range and environmental persistence. It difficult to control because its genetic versatility, expressed in the many phenotypical differences among isolates. genomes B. B05.10 T4 strains have been recently sequenced, becoming model system for pathogens, thus opening new alternatives functional genomics analysis. In this work, mycelium secreted proteome six wild-type with different range, grown liquid...
Abstract Lasiodiplodia theobromae (Botryosphaeriaceae, Ascomycota) is a plant pathogen and human opportunist whose pathogenicity modulated by temperature. The molecular effects of temperature on L . are mostly unknown, so we used multi-omics approach to understand how affects the mechanisms pathogenicity. genome LA-SOL3 was sequenced (Illumina MiSeq) annotated. Furthermore, transcriptome TruSeq) proteome (Orbitrap LC-MS/MS) grown at 25 °C 37 were analysed. Proteins related (plant cell wall...
Summary Orobanche crenata (broomrape) is an important constraint to pea ( Pisum sativum ) cultivation in the Mediterranean area, because little resistance available commercial crop varieties. Field experiments have demonstrated that some present a number of P. and fulvum accessions. The goal this work was characterize such resistance. – O. interaction symptoms were studied under controlled conditions by using Petri dish polyethylene bag assays. content phenolics peroxidase activity host...
Abstract As a first approach in establishing the holm oak leaf proteome, we have optimised protocol for this plant and tissue which includes following steps: trichloroacetic acid‐acetone extraction, two‐dimensional gel electrophoresis (2‐DE) on pH 5 to 8 linear gradient immobilised strips as dimension, sodium dodecyl sulfate‐polyacrylamide 13% polyacrylamide gels second one. Proteins were detected by Coomassie staining. Gel images recorded digitalized, protein spots quantified using...
Major proteins of the holm oak leaf proteome have been previously identified using a combination 2-DE, MS analysis and BLAST similarity search (Jorge et al., Proteomics 2005, 5, 222-234). That study, conducted with field samples from mature trees, revealed existence great variability in 2-DE protein map, qualitative as well quantitative changes, both analytical biological. A similar study has carried out 2-year-old seedlings to analyze study: (i) changes profile at different tree...
As a global approach to gain better understanding of the mechanisms involved in pea resistance Erysiphe pisi, changes leaf proteome two genotypes differing their phenotype were analyzed by combination 2-DE and MALDI-TOF/TOF MS. Leaf proteins from control non-inoculated inoculated susceptible (Messire) resistant (JI2480) plants resolved 2-DE, with IEF 5-8 pH range SDS-PAGE on 12% gels. CBB-stained gels revealed existence quantitative qualitative differences between extracts from: (i) leaves...
Sunflower broomrape (Orobanche cumana Wallr.) is a root holoparasitic angiosperm considered as one of the major constraints for sunflower production in Mediterranean areas. Breeding resistance regarded most effective, feasible, and environmentally friendly solution to control this parasite. However, existing sources genetic are defeated by continuous emergence new more virulent races In work, interaction between O. has been analysed order gain insights into mechanisms involved resistance....