A fast and simple method with CZE coupled to ESI/QTOF-MS was optimized validated for quantitative determination of organic acids (lactic acid, succinic malic tartaric shikimic citric acid) in red wines. The BGE ammonium acetate the separation analytes performed a polybrene-coated capillary presence EOF. sample preparation included dilution filtration wine. showed satisfactory performance characteristics: good linearity each correlation coefficients ranging from r2 = 0.9902 (shikimic 0.9990...

We established a new reversed phase-high performance liquid chromatography method combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry for the simultaneous determination and structural characterization of different lipid A types in bacteria (Escherichia coli O111, Salmonella adelaide O35 Proteus morganii O34) showing serological cross-reactivity. The complex mixtures (obtained by simple extraction acid hydrolysis outer membrane lipopolysaccharides) were...

Non-phosphorylated lipid A species confer reduced inflammatory potential for the bacteria. Knowledge on their chemical structure and presence in bacterial pathogens may contribute to understanding of resistance activation host innate immune system. In this study, we report fragmentation pathways negatively charged, non-phosphorylated under low-energy collision-induced dissociation conditions an electrospray ionization quadrupole time-of-flight instrument. Charge-promoted consecutive...

Abstract In this study, we report the detailed analysis of fragmentation patterns positively charged lipid A species based on their tandem mass spectra obtained under low‐energy collision‐induced dissociation conditions an electrospray quadrupole time‐of‐flight spectrometer. The spectrometry experiments were performed after separation compounds with a reversed‐phase high performance liquid chromatography method. We found that both, phosphorylated and nonphosphorylated molecules can be...

Lipid A represents a heterogeneous group of bacterial outer membrane phosphoglycolipids, which play major role in the pathogenesis Gram-negative sepsis. The number and position phosphoryl acyl groups lipid molecules are key structural determinants their bioactivities. In this study, NACE-ESI-MS/MS method was developed for simultaneous analysis isomers possessing different degree phosphorylation acylation. Various C4'- C1-monophosphorylated isobars, as well acylation isomers, were baseline...

Jipi-japa (Carludovica palmata) and zamia (Zamia dressleri) are endemic species found mostly in the forests of Central America including Panamá. In theory both jipi-japa fruit stem seeds edible, although were only consumed occasionally before modern times. The complete detailed carotenoid analysis fruits brown leaves was achieved using HPLC-DAD-MS technique, co-chromatography with authentic samples. Plant extracts subjected to HPLC separation on C30 C18 columns make possible a better...

Pseudomonas aeruginosa is a priority target pathogen for antibiotic research and development because of its high resistance to wide range antibiotics. Acquisition sometimes associated with modifications the lipid A component present in outer membrane most Gram-negative bacteria. For deeper understanding subtle chemical variations relation biological properties, structural elucidation species within bacterial strain crucial importance. However, simultaneous presence monophosphorylated...

The toxic nature of bacterial endotoxins is affected by the structural details lipid A, including variety and position acyl chains phosphate group(s) on its diglucosamine backbone. Negative-ion mode tandem mass spectrometry a primary method for structure elucidation used independently or in combination with separation techniques. However, it challenging to accurately characterize constitutional isomers A extracts direct spectrometry, as elemental composition molecular these molecules are...

n-Hexane, chloroform, ethyl acetate and 50% ethanol in water extracts prepared from the air-dried flowering parts of Lythrum salicaria L. were tested for vitro pharmacological properties on Guinea-pig ileum, which is suitable detecting a whole range neuronal smooth muscle effects. UHPLC-MS was used to evaluate polyphenol components extracts. In most prominent response (46.4% related 0.5 microM histamine) causing contractions triggered by extract concentration-dependent manner. Atropine,...

Lutein and its cis-isomers occur in a lot of plants, including variety flowers. In this study, lutein isomers were produced via iodine-catalyzed isomerization, four (9Z-, 9'Z-, 13Z-, 13Z') isolated by means column chromatography semipreparative HPLC. The structures the 9'Z- 13'Z-isomers elucidated NMR measurements. These compounds used as standards for HPLC-DAD-MS determination carotenoid composition flowers 20 plant species, which geometrical are main components. showed great variation...

The carotenoid composition of petals, florets, and full inflorescences Calendula officinalis grown in sun shade was investigated by the HPLC-DAD-MS method. total content flowers higher than those (1.154 0.872 mg/g petals), while no differences were found proportion individual carotenoids. In all samples, 29 components identified, from which main carotenoids, besides lutein, 5,8-epoxy-carotenoids with 8S 8′R configurations. 5,8-epoxy-carotenoid chrysanthemaxanthin configuration not...

Abstract Capillary isoelectric focusing hyphenated with mass spectrometry detection, following the sequential injection of carrier ampholytes and sample zone, is highly efficient for characterization proteins. The main advantage protocol that ampholytes, pH ranges, which are not supposed to cover points components, can be used separation. method then allows online detection separated analytes either in absence (substances have left gradient) or presence low‐level migrating within gradient)....

This paper presents the genome sequence of a Shigella sonnei mutant strain (S. 4351) and effect mutation in lipopolysaccharide biosynthesis on bacterial fitness. Lipopolysaccharides are major component outer leaflet Gram-negative membrane. We report here frameshift gene gmhD S. 4351. The results lack epimerization core heptose while we also found increased thermosensitivity, abnormal cell division, susceptibility to erythromycin cefalexin compared 4303. Comparative genomic analysis...

Lipid A, the inflammatory portion of lipopolysaccharides (LPS, endotoxins), is main component outer membrane Gram-negative bacteria. Its bioactivity in humans and animals strictly related to its chemical structure. In present work, fragmentation patterns singly charged monosodium [M + Na]+ disodium - H 2Na]+ adducts, as well protonated form monophosphorylated lipid A species were investigated detail using positive-ion electrospray ionization-based tandem (MS/MS) multistage mass spectrometry...

Abstract For an ensemble of particles with selfconsistent interaction we consider two models the matter for describing time evolution system: kinetic and fluid model given by Vlasov-Poisson system Euler-Poisson pressure zero. Suppose that in description velocities initially concentrate. We then show also concentrate later times. In hydrodynamical limit this concentration represents a solution dynamical model.

The structure of the oligosaccharide repeating units endotoxins from Gram‐negative bacteria is characteristic for different serogroups and serotypes bacteria. Detailed examination cross‐reactions three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, Salmonella enterica sv. Adelaide O35, was performed using sensitive tests (ELISA, immunoblotting). Fine differences between were detected silver staining SDS‐PAGE gels chip‐technology intact lipopolysaccharides (LPSs)....