A5083301448- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Lipid metabolism and biosynthesis
- Vitamin C and Antioxidants Research
- Vitamin K Research Studies
- Biofuel production and bioconversion
- Enzyme Structure and Function
- Plant biochemistry and biosynthesis
- Carbohydrate Chemistry and Synthesis
- Phytase and its Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Rocket and propulsion systems research
- Food Industry and Aquatic Biology
- Alcoholism and Thiamine Deficiency
- Seaweed-derived Bioactive Compounds
- Plant and animal studies
- Biochemical Acid Research Studies
- Thermal and Kinetic Analysis
- Chemical Thermodynamics and Molecular Structure
- Metabolism and Genetic Disorders
- Amino Acid Enzymes and Metabolism
- Insect and Arachnid Ecology and Behavior
- Advanced Chemical Physics Studies
- Advanced Combustion Engine Technologies
Microbiology Institute of Shaanxi
2018-2024
Institute of Microbiology
2018-2022
Chinese Academy of Sciences
2018-2022
Czech Academy of Sciences, Institute of Microbiology
2022
University of Chinese Academy of Sciences
2019-2021
State Key Laboratory of Microbial Resources
2018-2021
Yangzhou University
2020
Simon Fraser University
2011
Acetyl-CoA is an important metabolic intermediate and serves as acetylation precursor for the biosynthesis of various value-added acetyl-chemicals. can be produced from glucose, acetate, or fatty acids via pathways in Escherichia coli. Although glucose efficient carbon source acetyl-CoA production, pathway acetate to shortest produce through acid oxidation along with abundant NADH FADH2. In this study, metabolically engineered E. coli strains efficiently supplying were constructed applied...
Abstract Background The biosynthesis of high value-added compounds using metabolically engineered strains has received wide attention in recent years. Myo-inositol (inositol), an important compound the pharmaceutics, cosmetics and food industries, is usually produced from phytate via a harsh set chemical reactions. Recombinant Escherichia coli have been constructed by metabolic engineering strategies to produce inositol, but with low yield. proper distribution carbon flux between cell growth...
Inspired by the catalytic mechanism of cytochrome P450 enzymes, we have evolved a fatty acid hydroxylase, P450BSβ, into decarboxylase after four rounds saturation mutagenesis that now predominantly converts acids to 1-alkenes. The engineered enzyme directly utilizes inexpensive H2O2 oxidatively decarboxylate C6–C18 saturated as well plant oil-derived unsaturated acids, displaying high reactivity (e.g., 3,700 h–1 turnover frequency for myristic acid). With in situ supplied glucose oxidase,...
Menaquinone-7 (MK-7) possesses wide health and medical value, the market demand for MK-7 has increased. Metabolic engineering production in Escherichia coli still remains challenging due to characteristics of competing quinone synthesis, cells mainly synthesized menaquinones under anaerobic conditions. To increase engineered E. strains aerobic conditions, we divided whole biosynthetic pathway into three modules (MVA pathway, DHNA pathway) systematically optimized each them. First, by...
The string of bacteria, Vibrio. sp. QD-5 utilizing alginate, was separated from rotten kelp. results genome sequencing showed that the strain contained four alginate lyase genes.One genes Aly-IV cloned and linked to plasmid pET-22b (+). heterologous expressed aly-IVwas characterized，which possessed theoretical molecular mass 62 kDa, isoelectric point (pI) 5.12. - enzyme aly-IV purified activity reached 1256.78 U/mg, with optimal temperature 35 oC pH value 8.9. Nurtured in below 25 for 30...
The inventory of labeled fatty acids and protocols for their syntheses are constantly increasing, but site‐specific precursors in biosynthetic studies still needed. Ethyl oleate (EO) is an important primer pheromone honeybees, which responsible the regulation behavioral maturation. During our on EO, a oleic acid precursor was required. In this report, synthetic route adaptable to preparation [9,10,11,11‐D 4 ] its derived esters use as tracers EO metabolism honey bees presented.
Sulfur-containing fuels, such as petroleum natural gas, and biofuels, produce SO2, SO3, other highly toxic gases upon combustion, which are harmful to human health the environment, making it essential understand their thermochemical properties. This study used high-level quantum chemistry calculations determine thermodynamic parameters, including entropy, enthalpy, specific heat capacity for an extensive set of sulfur-containing species. The B3LYP/cc-pVTZ level theory was geometry...
Abstract Modular co-culture engineering has been applied to divide labor among different strains in the biomanufacturer of substances through complicated metabolic pathways. However, dynamic instability, uncontrollable population ratios, and lack mathematical models have hindered its further application. In this study, we built a model used it design self-stable system with adjustable ratios two engineered Escherichia coli strains. As an indicator product, yield chlorogenic acid was...