A5084876444- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Congenital heart defects research
- Single-cell and spatial transcriptomics
- Electrospun Nanofibers in Biomedical Applications
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Cardiac electrophysiology and arrhythmias
- Muscle Physiology and Disorders
- Cellular Mechanics and Interactions
- Ion Transport and Channel Regulation
- Electrohydrodynamics and Fluid Dynamics
- Protein Degradation and Inhibitors
- Signaling Pathways in Disease
- Mesenchymal stem cell research
- Cardiac Fibrosis and Remodeling
- Ion channel regulation and function
Duke University
2019-2025
The University of Queensland
2017-2022
Research Institute for Bioscience and Biotechnology
2017
Candidate cardiomyocyte (CM) mitogens such as those affecting the extracellular signal–regulated kinase (ERK) signaling pathway represent potential targets for functional heart regeneration. We explored whether activating ERK via a constitutively active mutant of B-raf proto-oncogene (BRAF), BRAF-V600E (caBRAF), can induce proproliferative effects in neonatal rat engineered cardiac tissues (ECTs). Sustained CM-specific caBRAF expression induced chronic activation, substantial tissue growth,...
Abstract Notch signaling plays a pivotal role in regulating satellite cell (SC) behavior during skeletal muscle development, homeostasis, and repair. While well‐characterized mouse models, the impact of human tissues remains largely underexplored. Here, 3D tissue‐engineered model (“myobundles”) is utilized as an vitro platform for temporal control studies singaling. Myofiber‐specific overexpression ligand, DLL1, early myobundle differentiation increases abundance SCs shifts their phenotype...
Abstract Differentiation into diverse cell lineages requires the orchestration of gene regulatory networks guiding fate choices. Utilizing human pluripotent stem cells, we measured expression dynamics 17,718 genes from 43,168 cells across five time points over a thirty day time-course in vitro cardiac-directed differentiation. Unsupervised clustering and lineage prediction algorithms were used to map choices transcriptional underlying cardiac We leveraged this resource identify strategies...
Histone deacetylases (HDACs) are a class of enzymes that control chromatin state and influence cell fate. We evaluated the accessibility transcriptome dynamics zinc-containing HDACs during differentiation in vitro coupled with chemical perturbation to identify role mesendoderm fate specification. Single-cell RNA sequencing analyses HDAC expression human pluripotent stem (hPSC) mouse gastrulation vivo reveal unique association HDAC1 -3 gene programs exit from pluripotency. Functional small...
Background: Alternating hemiplegia of childhood (AHC) is a rare de novo syndrome that manifests with episodic hemiplegia, seizures, dystonia, and, notably, sudden unexplained death. Gene positive patients carry pathogenic variant in the ATP1A3 -encoded Na/K ATPase alpha 3 isoform (ATP1A3), and D801N most common variant. Our group recently found AHC ATP1A3-D801N missense have short QTc are at risk ventricular fibrillation. Hypothesis: We hypothesized results reduced function leading to Ca 2+...
Differentiation into diverse cell lineages requires orchestration of gene regulatory networks guiding fate choices. Here, we present the dissection cellular composition and from transcriptomic data 43,168 cells across five discrete time points during cardiacdirected differentiation. We utilize unsupervised clustering implement a lineage trajectory prediction algorithm that integrates transcription factor to predict progression 15 subpopulations correlate with germ layer cardiovascular...