A5044570980- Influenza Virus Research Studies
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- RNA and protein synthesis mechanisms
- Immune Response and Inflammation
- Protein Structure and Dynamics
- interferon and immune responses
- Viral Infections and Vectors
- Bacteriophages and microbial interactions
- COVID-19 epidemiological studies
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Virology and Viral Diseases
- Receptor Mechanisms and Signaling
- SARS-CoV-2 and COVID-19 Research
- Lipid Membrane Structure and Behavior
- Metabolomics and Mass Spectrometry Studies
- vaccines and immunoinformatics approaches
- Virus-based gene therapy research
- Extracellular vesicles in disease
- Advanced Electron Microscopy Techniques and Applications
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Eicosanoids and Hypertension Pharmacology
Ministry of Health of the Russian Federation
2015-2025
D.I. Ivanovsky Institute of Virology Russian Academy of Medical Sciences
2007-2023
Gamalei Institute of Epidemiology and Microbiology
2018-2021
Institute of Virology of the Slovak Academy of Sciences
2019
Newcastle disease viruses (NDVs) circulating among wild birds and poultry may differ in virulence. Some NDVs cause devastating outbreaks chickens. The NDV/duck/Moscow/3639/2008 (d3639) strain was isolated from a duck. Its genome sequenced (PP795281, GenBank) the biological properties, specifically for infection chicken mice, were studied. Strain d3639 of genotype I.2 has an F protein cleavage site (112-GKQGRL-117) HN length (616 a.a.) lentogenic pathotype. It tested, comparison with II...
The highly pathogenic avian influenza H5N1 viruses have become widespread and evolved into several clades. In our previous studies, the antigenic sites of H5 hemagglutinin (HA) were characterized by selection sequencing escape mutants. present studies we analyzed epitopes recognized monoclonal antibodies against A/Duck/Novosibirsk/56/05 (H5N1) virus isolated in western Siberia belonging to subclade 2.2 viruses. analysis revealed antigenically relevant positions amino acid residues globular...
Topological rearrangements of biological membranes, such as fusion and fission, often require a sophisticated interplay between different proteins cellular membranes. However, in the case enveloped viruses, even one molecule can execute membrane restructurings. Growing evidence indicates that matrix viruses solely trigger bending required for another crucial step virogenesis, budding progeny virions. For influenza A virus protein M1, studies report both favor against M1 being able to produce...
Antigenic mapping of the haemagglutinin (HA) molecule H5 and H9 influenza viruses by selecting escape mutants with monoclonal anti-HA antibodies subjecting selected to immunological analysis sequencing has previously been performed. The used as wild-type strains were mouse-adapted variants original isolates. Phenotypic characterization revealed that amino acid change in HA conferred resistance a antibody was sometimes associated additional effects, including decreased virulence for mice. In...
Newcastle disease virus (NDV) is an enveloped paramyxovirus. The matrix protein of the (M-NDV) has innate propensity to produce virus-like particles budding from plasma membrane expressing cell without recruiting other viral proteins. predominantly infects host via fusion with or, alternatively, can use receptor-mediated endocytic pathways. question arises as what are mechanisms supporting such diversity, especially concerning assembling and binding properties scaffold under both neutral...
Purpose: The aminoadamantane derivative of L-histidyl-1-adamantayl ethylamine hydrochloride (HCl*H-His-Rim) has showed a high inhibition level against influenza A virus strains in vitro. aim this work is to search and establish evidence the direct effect drug on proton channel M2. Methods: compound HCl*H-His-Rim was obtained by classical peptide synthesis methods. Influenza mutants A/PuertoRico/8/34(H1N1) strain were reverse genetics mutant samples cultured chicken embryos with titer...
Mutations arising in influenza viruses that have undergone immune pressure may promote a successful spread of mutants nature.In order to evaluate the variability nonpathogenic virus A/duck/Moscow/4182-C/2010(H5N3) and determine common epitopes between it highly pathogenic H5N1 avian (HPAIV), set escape was selected due action MABs specific against A/chicken/Pennsylvania/8125/83(H5N2), A/Vietnam/1203/04(H5N1) A/duck/ Novosibirsk/56/05(H5N1) viruses.The complete genomes were sequenced amino...
The influenza A virus remains one of the most common and dangerous human health concerns due to its rapid evolutionary dynamics. Since changes viruses can be traced in real time, last decade has seen a surge research on an increase experimental data (selection escape mutants followed by examination their phenotypic characteristics generation with desired mutations using reverse genetics). Moreover, advances our understanding are also attributable development new computational methods based...
The North American low pathogenic H7N2 avian influenza A viruses, which lack the 220-loop in hemagglutinin (HA), possess dual receptor specificity for avian- and human-like receptors. purpose of this work was to determine amino acid substitutions HA affect viral antigenic phenotypic properties that may be important virus evolution. By obtaining escape mutants under immune pressure treatment with monoclonal antibodies, antigenically acids were determined at positions 125, 135, 157, 160, 198,...
Introduction. The WHO regularly updates influenza vaccine recommendations to maximize their match with circulating strains. Nevertheless, the effectiveness of A vaccine, specifically its H3N2 component, has been low for several seasons.
 aim study is develop a mathematical model cross-immunity based on array published hemagglutination inhibition assay (HAI) data.
 Materials and methods. In this study, was proposed, finding, using regression analysis, dependence HAI titers...
Cryo-electron microscopy (cryo-EM) is indispensable for the structural studies of enveloped viruses – dangerous pathogens humans and animals. Yet, it requires highly specialized equipment as well careful sample preparation. In this work, capabilities transmission electron microscope JEOL JEM-2100 equipped with cryo-transfer holder are used, preliminary cryo-EM data influenza A B virus strains SARS-CoV-2 inactivated beta-propiolactone presented. Image analysis allows: (1) to distinguish...
Avian influenza viruses of the H1 and H5 subtypes were involved in formation highly pathogenic that caused pandemics panzootics 20th–21st centuries. In order to assess zoonotic potential these subtypes, two H1N1 H5N3 have been isolated from wild ducks Moscow adapted for growth mouse lungs. Their phenotypic properties studied, genetic changes occurred during adaptation identified. The original A/duck/Moscow/4970/2013 (H1N1) A/duck/Moscow/4182-C/2010 (H5N3) apathogenic mice but became after...
The change in the phenotypic properties resulting from amino acid substitutions hemagglutinin (HA) molecule is an important link evolutionary process of influenza viruses. It believed to be one mechanisms emergence highly pathogenic strains A viruses, including subtype H5N1. Using site-directed mutagenesis, we introduced mutations HA gene H5N1 virus. obtained virus variants were analyzed and compared using following parameters: optimal pH conformational transition (according results...
Introduction. H7 subtype avian influenza viruses causing severe epizootics among birds are phylogenetically different in the Eastern and Western hemispheres. Numerous human infections caused by these hemisphere indicate that can overcome interspecies barrier pose a potential threat of new pandemic.The H7N2 with deletion amino acids 221–228 (H3 numbering) hemagglutinin (HA) had been circulating poultry Hemisphere during 1996–2006, once again detected 2016 an animal shelter, where they cat...